Article Per Year (5 Year)
p-Index From 2020 - 2025
0.659
P-Index
This Author published in this journals
All Journal Jurnal Biodjati Biofaal Journal
Apituley, Edwin Thomas
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Materials Science & Nanotechnology Medicine & Pharmacology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
ANALISIS FILOGENETIK GEN gyrA DAN gyrB DARI GENUS MESORHIZOBIUM, RHIZOBIUM DAN ENSIFER Apituley, Edwin Thomas; Samson, Efraim; Killay, Amos
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp118-127

Abstract

Nucleotide sequence of gyrA and gyrB genes encoding subunit A and B of gyrase enzyme from genus of Rhizobium, Ensifer and Mesorhizobium were analyzed to determine evolutionary relationship among them. Maximum Likelihood and Kimura 2 parameter methods were used to construct phylogenetic tree and measure genetic distance. Phylogenetic tree based on nucleotide sequence of gyrA gene was compared to phylogenetic tree based on nucleotide sequence of gyrB gene. Comparison of identity percentage among nucleotide sequence of gyrA gene from genus Rhizobium, Ensifer and Mesorhizobium show similarity, where each of them have narrow range of identity percentage, however nucleotide sequence of gyrB gene from genus Rhizobium and Ensifer show wider identity percentage range than genus Mesorhizobium. Several member of genus Rhizobium and Ensifer have double copy of gyrB gene with identity percentage less than 50 percent between them. Topology of phylogenetic tree based on nucleotide sequence of gyrA gene have similar topology to topology of phylogenetic tree based on nucleotide sequence of gyrB gene, except for additional branch formed by one of additional copy of gyrB sequences. .
ANALISIS FILOGENETIK SUBUNIT ALFA DNA POLIMERASE III DAN ERROR-PRONE DNA POLIMERASE DARI GENUS RHODOBACTER Apituley, Edwin Thomas; Killay, Amos
Biofaal Journal Vol 3 No 1 (2022): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i1pp19-27

Abstract

Sekuens nukleotida dari gen dnaE yang mengkodekan subunit alfa dari DNA polimerase III maupun sekuen asam amino produknya dari genus Rhodobacter dan beberapa genus yang berhubungan dianalisis untuk menentukan hubungan evolusioner mereka. Metode Maximum Likelihood dan model Kimura 2 parameter masing-masing untuk mengkonstruksi pohon filogenetik dan mengukur jarak genetik. Pohon filogenetik berdasarkan sekuen nukleotida dari gen yang mengkodekan subunit alfa dari DNA polimerase III dibandingkan terhadap pohon filogenetik berdasarkan sekuen gen 16S rRNA, Genus Rhodobacter maupun genus lain yang diuji memiliki 2 jenis dnaE, diberi nama dnaE1 dan dnaE2. Perbandingan pasangan sekuen menunjukkan bahwa persentase keidentikan sekuen antara kedua jenis gen dibawah 50 persen untuk sekuens nukleotida dan 25 persen untuk sekuen asam amino. Satu anggota genus Rhodobacter, R.sediminicola JA983 memiliki tiga salinan gen dnaE dengan rentang keidentikan 40 persen hingga 54 persen untuk sekuen nukleotida dan 22.6 hingga 50.6 persen untuk sekuen asam amino. Pohon filogenetik berdasarkan sekuen nukleotida dnaE memiliki dua percabangan utama, masing-masing mewakili dnaE1 dan dnaE2. Topologi dari pohon filogenetik berdasarkan gen dnaE memiliki kemiripan terhadap pohon filogenetik berdasarkan sekuen gen 16S rRNA, terutama untuk percabangan dnaE1. R.sediminicola memiliki satu salinan gen pada percabangan dnaE1 dan dua salinan gen pada percabangan dnaE2, pada pohon filogenetik berdasarkan sekeun gen dnaE, dan dianotasi sebagai dnaE2.1 dan dnaE2.2.
Bioprospecting of Potential Microorganisms as Agents for Biodiesel Production from Sago Pulp Waste Feedstock Lethulur, Alice Chaela; Seumahu, Cecilia Anna; Apituley, Edwin Thomas
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.48431

Abstract

The rising global energy demand and environmental issues associated with fossil fuel use have accelerated interest in renewable alternatives, such as biodiesel. This study aimed to provide a preliminary screening of potential indigenous microorganisms among isolates with high ability to saccharify lignocellulosic sago pulp waste, an abundant, non-food biomass in Maluku, and to accumulate lipids. Laboratory experiments were conducted to isolate, characterize (macro- and microscopically), saccharify, and accumulate lipids. Bacterial isolates were evaluated for saccharification ability using two parameters: the cellulolytic index (CI), determined by the clear zone on solid CMC media, and reducing sugar production in modified liquid CMC media using pretreated sago waste, measured by Benedict’s test. Yeast and mold isolates were assessed for lipid accumulation through Sudan Black B staining and gravimetric lipid extraction under nitrogen-limited conditions. Results identified four bacterial isolates capable of saccharification, with isolate BAS 1B showing the highest cellulolytic index, and all isolates produced detectable reducing sugars by Benedict’s test. All yeast and mold isolates accumulated intracellular lipids, with YAS 2 and KAS 2 isolates accumulating the highest lipid compared to the positive control (Saccharomyces cerevisiae). As this study was based on a single-point observation (n=1), the results are exploratory and should be interpreted cautiously. Nevertheless, isolates BAS 1B, YAS 2, and KAS 2 show promising potential for further biodiesel-related research using lignocellulosic waste. Further studies with replication and quantitative validation are required prior to any industrial consideration
Co-Authors Amos Killay CECILIA ANNA SEUMAHU Efraim Samson, Efraim Lethulur, Alice Chaela