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All Journal HAYATI Journal of Biosciences Jurnal Biodjati Unram Journal of Community Service (UJCS) Biofaal Journal Rumphius Pattimura Biological Journal Innovation for Community Service Journal
CECILIA ANNA SEUMAHU
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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ANTIBACTERIAL ACTIVITY AND INHIBITION OF BIOFILM OF Hibiscus tiliaceus STEM BARK METHANOL EXTRACT TO Streptococcus mutans GROWTH Lukaraja, Wati; Lessy, Widya; Seumahu, Cecilia Anna; Pesik, Anneke
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p066-071

Abstract

Dental caries is an infectious disease that occurs in the oral cavity preceded by the formation of dental plaque or biofilm. This study aims to examine the inhibition and biofilm inhibition of hibiscus tree bark extract (Hibiscus tiliaceus). The test methods used were Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) while the analysis of biofilm inhibition used UV-VIS spectrophotometry at a wavelength of 580 nm with McFarlandII standard (6x108 CFU/ml). The results showed that the methanol extract of hibiscus bark at a small concentration of 0.5% could inhibit and kill Streptococcus mutans as indicated by the number of colonies that grew less with a value of 3.34 x 107 CFU/ml in colonies that grew at an extract concentration of 2. % and 1%. Biofilm inhibition is shown by the decreasing absorbance value due to the increased extract concentration so that less biofilm formation occurs.
Bacterial and Fungal Communities in Tempeh as Reveal by Amplified Ribosomal Intergenic Sequence Analysis SEUMAHU, CECILIA ANNA; SUWANTO, ANTONIUS; RUSMANA, IMAN; SOLIHIN, DEDY DURYADI
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.073 KB) | DOI: 10.4308/hjb.20.2.65

Abstract

Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles.  However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.
Pelatihan Pengamatan Sel Dengan Mikroskop Olympus Cx-21 dan Dokumentasi Sel Menggunakan Smartphone Bagi Siswa Dan Guru Ma Al-Muluuk Persis Telaga Kodok, Leihitu, Kabupaten Maluku Tengah Seumahu, Cecilia Anna; Leimena, Handy Erwin Pier; Sahertian, Dece Elisabeth; Ukratalo, Abdul Mahid
Innovation for Community Service Journal Vol 2 No 1 (2024): April 2024
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/icsj.v2i1.12486

Abstract

Penggunaan mikroskop sebagai alat dalam praktikum biologi menjadi sangat penting karena dapat mendukung keterampilan proses belajar sains. Tujuan dari kegiatan ini adalah untuk memberikan pemahaman yang mendalam kepada siswa dan guru mengenai penggunaan mikroskop Olympus CX-21 untuk pengamatan sel serta penggunaan smartphone untuk dokumentasi sel. Metode yang digunakan adalah ceramah, diskusi, tanya jawab serta demonstrasi untuk menyiapkan preparat segar tumbuhan, menggunakan mikroskop dengan benar serta mendokumentasi hasil pengamatan. Hasil yang dicapai adalah peserta mampu menggunakan mikroskop dan menyiapkan preparat segar tumbuhan dan mendokumentasikan serta memvisualisasikan hasil pengamatan. Peserta terlihat sangat antusias dan tertarik karena mampu mengamati jaringan tumbuhan langsung di bawah mikroskop.
SKRINING FITOKIMIA DAN ANTIBAKTERI CANGKANG DAN DURI BULU BABI Diadema setosum Birahy, Deford Cristy; Siahaya, Griennasty Clawdya; Bustomi, Bustomi; Seumahu, Cecilia Anna; Nindatu, Maria; Mainassy, Meillisa C; Huwae, Laury Marcia Ch
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp044-052

Abstract

Bulu babi Diadema setosum relatif melimpah di Indonesia. Penelitian ini bertujuan untuk mengidentifikasi senyawa fitokimia dan kemampuan antibakteri ekstrak etanol cangkang dan duri bulu babi Diadema setosum terhadap isolat bakteri Escherichia coli, Staphylococcus aureus, dan Salmonella sp. Metode penelitian yang dilakukan meliputi ekstraksi cangkang dan duri bulu babi Diadema setosum dengan cara maserasi menggunakan etanol 96%, kemudian dilakukan skrining fitokimia dan uji aktivitas antibakteri dengan metode difusi sumur. Hasil penelitian menunjukkan bahwa skrining fitokimia ekstrak cangkang bulu babi (Diadema setosum) mengandung senyawa alkaloid, flavonoid, dan saponin. Senyawa yang ditemukan pada ekstrak duri bulu babi Diadema setosum antara lain alkaloid, flavonoid, dan tanin. Pengujian antibakteri dilakukan dengan menggunakan metode difusi. Hasil uji aktivitas antibakteri menunjukkan bahwa kedua sampel (cangkang dan duri) menghasilkan zona hambat terhadap isolat bakteri Escherichia coli, Staphylococcus aureus, dan Salmonella sp.
Introducing Indonesian endemism, its threat and conservation solution for SMA Negeri 10 students in Kamarian Village, West Seram Regency, Maluku Leimena, Handy Erwin Pier; Seumahu, Cecilia Anna; Sahertian, Dece Elizabeth; Ukratalo, Abdul Mahid; Kaihena, Martha
Unram Journal of Community Service Vol. 5 No. 1 (2024): March
Publisher : Pascasarjana Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ujcs.v5i1.585

Abstract

West Seram Regency on Seram Island in Maluku Province, Indonesia has extraordinary biodiversity and is the habitat of several endemic species of Maluku and Indonesia. As a form of implementation of the dharma of community service, socialization was conducted with students of SMA Negeri 10 in Kamarian Village, West Seram Regency, Maluku about Indonesian endemism, its threats and conservation. The socialization was attended by 38 students. The socialization results indicate that 91% of students demonstrated a good understanding of endemic species, and 82% of students obtained information on endemic species from this activity. Additionally, students were able to identify 11 Indonesian endemic species, as well as the threats and efforts to protect them. This activity has the potential to increase environmental awareness among school students, the nation's young generation.
Bioprospecting of Potential Microorganisms as Agents for Biodiesel Production from Sago Pulp Waste Feedstock Lethulur, Alice Chaela; Seumahu, Cecilia Anna; Apituley, Edwin Thomas
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.48431

Abstract

The rising global energy demand and environmental issues associated with fossil fuel use have accelerated interest in renewable alternatives, such as biodiesel. This study aimed to provide a preliminary screening of potential indigenous microorganisms among isolates with high ability to saccharify lignocellulosic sago pulp waste, an abundant, non-food biomass in Maluku, and to accumulate lipids. Laboratory experiments were conducted to isolate, characterize (macro- and microscopically), saccharify, and accumulate lipids. Bacterial isolates were evaluated for saccharification ability using two parameters: the cellulolytic index (CI), determined by the clear zone on solid CMC media, and reducing sugar production in modified liquid CMC media using pretreated sago waste, measured by Benedict’s test. Yeast and mold isolates were assessed for lipid accumulation through Sudan Black B staining and gravimetric lipid extraction under nitrogen-limited conditions. Results identified four bacterial isolates capable of saccharification, with isolate BAS 1B showing the highest cellulolytic index, and all isolates produced detectable reducing sugars by Benedict’s test. All yeast and mold isolates accumulated intracellular lipids, with YAS 2 and KAS 2 isolates accumulating the highest lipid compared to the positive control (Saccharomyces cerevisiae). As this study was based on a single-point observation (n=1), the results are exploratory and should be interpreted cautiously. Nevertheless, isolates BAS 1B, YAS 2, and KAS 2 show promising potential for further biodiesel-related research using lignocellulosic waste. Further studies with replication and quantitative validation are required prior to any industrial consideration
Co-Authors Anneke Pesik Antonius Suwanto Apituley, Edwin Thomas Birahy, Deford Cristy Bustomi Bustomi Handy Erwin Pier Leimena Iman Rusmana LAURY CHARA HUWAE Lessy, Widya Lethulur, Alice Chaela Lukaraja, Wati Mainassy, Meillisa C Martha Kaihena Nindatu, Maria Sahertian, Dece Elisabeth Sahertian, Dece Elizabeth Siahaya, Griennasty Clawdya TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Ukratalo, Abdul Mahid