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All Journal Journal of Agriculture (JoA) Jurnal Bioteknologi & Biosains Indonesia
Haswin, Dwi Wahyuni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Propagation of Shafira Taro (Colocasia Esculenta Var. Antiqourum) Applied TDZ, BAP, TDZ AND NAA, BAP and NAA In Vitro Haswin, Dwi Wahyuni; Haring, Fera; Riadi, Muh; Tambung, Astina; Yanti, Nur Aida; Ilham, Ilham
Journal of Agriculture Vol. 4 No. 01 (2025): Research Articles March 2025
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v4i01.5835

Abstract

Shafira taro (Colocasia esculenta var. antiquorum) is a type of small-tubered taro, also known as Japanese taro, which is traded internationally. This study aims to examine the effect of various concentrations of Thidiazuron (TDZ) and Benzyl Amino Purine (BAP), as well as the combination of Thidiazuron with Naphtalene Acetic Acid (NAA) and BAP with NAA, on the multiplication of Shafira taro shoots through in vitro culture techniques. The method used in this study uses in vitro culture techniques, which are modern plant propagation science known in the world of biotechnology and allow controlled propagation and improvement of plant species. An essential component of this technique is using plant growth regulators (PGRs), critical for modulating various physiological processes in plant cells and tissues. PGRs promote growth and differentiation, optimize secondary metabolite production, and increase plant resistance. This study used a combination of main PGR treatments (TDZ, BAP, and NAA) with a total of 13 (thirteen) treatment combinations using a randomized block design, namely: k0: Control, t1: TDZ 1 ppm, t2: TDZ 2 ppm, t3: TDZ 3 ppm, b1: BAP 1 ppm, b2: BAP 2 ppm, b3: BAP 3 ppm, t1n1: TDZ 1 ppm + NAA 0.5 ppm, t2n1: TDZ 2 ppm + NAA 0.5 ppm, t3n1: TDZ 3 ppm + NAA 0.5 ppm, b1n1: BAP 1 ppm + NAA 0.5 ppm, b2n1: BAP 2 ppm + NAA 0.5 ppm, b3n1: BAP 3 ppm + NAA 0.5 ppm. Each treatment was repeated thrice, with three tissue culture bottles per repetition, resulting in 117 tubes containing one explant. The results showed that the highest number of roots, leaves, and shoots was achieved with 1 ppm BAP, while the optimal fresh weight and shoot height were obtained with 2 ppm BAP. Treatments involving combinations of TDZ, TDZ + NAA, and BAP + NAA did not produce significant results for shoot multiplication.
Effect of Golden Snail-Based Local Microorganisms on the Growth and Yield of Baby Corn Aida Yanti, Nur; Anwar, Syaifuddin; H, Sudirman; Haswin, Dwi Wahyuni; Nasrul, Muhammad
Journal of Agriculture Vol. 4 No. 02 (2025): Research Articles July 2025
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v4i02.6688

Abstract

The use of local microorganisms (MOL) derived from golden apple snails (Pomacea canaliculata) is a sustainable agricultural innovation that supports plant growth through natural bioactivation. Golden apple snails, often considered pests in rice fields, are rich in protein and minerals, making them suitable substrates for microbial development. Fermenting these snails into MOL provides a natural source of beneficial microbes and nutrients that can improve soil health, enhance nutrient availability, and stimulate plant growth. This study aimed to evaluate the effect of different application doses of golden apple snail-based MOL on the growth and yield of semi-corn (Zea mays L.). The field experiment was conducted at Politeknik Pembangunan Pertanian Gowa using a Randomised Complete Block Design (RCBD) with four treatments and three replications. The treatments consisted of P0 = control (without MOL), P1 = 133 ml MOL/plant, P2 = 160 ml MOL/plant, and P3 = 186 ml MOL/plant. The observed parameters were plant height, number of leaves, ear length, and ear weight. The results showed that MOL application significantly affected plant height, ear length, and ear weight. Among the treatments, P2 produced the highest values for all observed parameters, including plant height (160.67 cm), ear length (13.95 cm), and ear weight (60.63 g). Therefore, a dose of 160 ml/plant of golden apple snail-based MOL is considered optimal for improving semi-corn growth and yield under field conditions.
GENETIC MUTATION INDUCTION OF Monstera adansonii ON VARIOUS MUTAGENS BY DRIPS APPLICATION Haswin, Dwi Wahyuni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1788

Abstract

Monstera adansonii has wide leaves with a split pattern on its strands (split leaf), smooth and shiny to create a basic visualization (background filler) in flower arrangements. This study aims to obtain genetic changes using chemical mutagens. Chemical mutagens used were 250 ppm EMS, 400 and 800 ppm streptomycin, 400 and 800 ppm GA3. The observations were performed after treatments on the parameter of amount of chlorophyll, stomata and leaf color changes. The results showed that both streptomycin treatments significantly reduced the plant height and the number of leaves. The application of streptomycin in high concentration (800 ppm) decreased the total amount of chlorophyll content in the leaves and changed the color as well as the shape of the Monstera leaves. All chemical mutagen treatments had no effect on stomatal density and stomatal area. The changes in leaf color and shape occurred in the high concentration of 800 ppm streptomycin treatment, could not detected by SSR marker.
Co-Authors Aida Yanti, Nur Anwar, Syaifuddin Astina Tambung, Astina H, Sudirman Haring, Fera Ilham Ilham Riadi, Muh Yanti, Nur Aida