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Phytochemical Screening of Suji (Pleomele angustifolia) Extract Bahri, Syamsul; Setiadi, Dadi; Lestari, Tri Ayu; Setiawan, Heru; Hakim, Muhammad Alfianul; Cahyadi, Lalu M. Zidan
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9560

Abstract

Phytochemical screening of Suji (Pleomele angustifolia) extract has been conducted. The aim of this research is to identify compounds and determine the antioxidant activity of suji  extract. The method used is laboratory experiments for phytochemical screening and antioxidant activity testing. This research consists of 2 (two) stages. The first stage aims to obtain extract of suji by using ethanol plus ethyl acetate and the second stage is carried out to test the content of secondary metabolite compounds using phytochemical tests and determine the antioxidant activity of extract of suji using the DPPH method. The extract of suji positively contains terpenoids, whereas flavonoid and  alkaloids are detetcted in a significant number. The DPPH test results obtained the equation y = 0.3492x + 12,059, indicating that the antioxidant activity of suji extract of  is classified as moderate as the IC50 value of the extract of suji is108,65 ppm
The Comparison of IC50 of Suji (Pleomele angustifolia) which is Extracted by Different Solvent Bahri, Syamsul; Setiadi, Dadi; Lestari, Tri Ayu; Setiawan, Heru; Hakim, Muhammad Alfianul; Cahyadi, Lalu M. Zidan
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9559

Abstract

The Determination both of IC50 of Suji (Pleomele angustifolia) and its phytochemical subtances content which extracted by a different solvent has been conducted. The aim of this research is to determine kind of solvent which achieve better result. The method used is laboratory experiments for phytochemical screening and antioxidant activity assay. This research consists of 2 (two) stages. First stage aims to obtain extract of suji by using certain solvent, and the second stage is carried out to examine  secondary metabolite compounds of the extract using phytochemical screening and then to determine the antioxidant activity of extract using the DPPH method. This research used  2 kinds of solvent, ethanol plus ethyl acetate in the first sample, and the second one by ethanol only. Sample which extracted by ethanol plus ethyl acetate positively contains terpenoids, whereas flavonoid and  alkaloids are detected in a significant number. The DPPH test results obtained the equation y = 0.3492x + 12,059, indicating that the antioxidant activity of suji which extracted by ethanol plus ethyl acetate is classified as moderate as the IC50 value of the extract of suji is108,65 ppm. The other sample which extracted just by ethanol also contains terpenoid, flavonoid, and alkaloid, but in a greater number. In the second sample also detected steroid compound which undetectable in the first sample. DPPH score in the second sample which extracted  by ethanol. is y= 06352x + 18, 786. By using this equation we found 49.14 ppm as IC50 score. This score is classified as strong activity. This result shows that antioxidant activity of the second extract is stronger than the first one. The result also meaning that ethanol is more effective in extracting phytochemical compounds of suji than ethanol plus ethyl acetate.
Abundance and Diversity of Spiders in Lemor Botanical Garden, East Lombok Hakim, Muhammad Alfianul; Ayun Pazira; Ariana Ashri Dewi; Baiq Hanifa Utami Mawan Putri
Journal of Biology, Environment, and Edu-Tourism Vol. 2 No. 1 (2026): April 2026
Publisher : Yayasan Siti Widhatul Faeha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65622/jbee.v2i1.265

Abstract

Spiders play an important role in ecosystems as natural predators and can be used as indicators of environmental conditions. This study aimed to analyze the relative abundance and diversity index of spiders in the ex-situ and in-situ conservation areas of Kebun Raya Lemor, East Lombok. Data collection was conducted using the Visual Encounter Survey (VES) method with a 200-meter transect and a 20-meter observation width. Data analysis was performed using the relative abundance formula and the Shannon–Wiener diversity index. The results showed that in the ex-situ conservation area, 13 species from 6 families were recorded, whereas in the in-situ conservation area, 8 species from 5 families were found. The highest relative abundance in the ex-situ area was represented by Leiobunum vittatum and Argyrodes miniaceus, while the in-situ area was dominated by Leiobunum vittatum. The diversity index in the ex-situ area was 2.32, and in the in-situ area was 1.32, both of which are classified as moderate; however, the ex-situ area showed higher diversity than the in-situ area. These findings indicate that the ex-situ area has a more heterogeneous habitat that supports a more diverse and balanced spider community than the in-situ area.