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Hakim, Muhammad Alfianul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Phytochemical Screening of Suji (Pleomele angustifolia) Extract Bahri, Syamsul; Setiadi, Dadi; Lestari, Tri Ayu; Setiawan, Heru; Hakim, Muhammad Alfianul; Cahyadi, Lalu M. Zidan
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9560

Abstract

Phytochemical screening of Suji (Pleomele angustifolia) extract has been conducted. The aim of this research is to identify compounds and determine the antioxidant activity of suji  extract. The method used is laboratory experiments for phytochemical screening and antioxidant activity testing. This research consists of 2 (two) stages. The first stage aims to obtain extract of suji by using ethanol plus ethyl acetate and the second stage is carried out to test the content of secondary metabolite compounds using phytochemical tests and determine the antioxidant activity of extract of suji using the DPPH method. The extract of suji positively contains terpenoids, whereas flavonoid and  alkaloids are detetcted in a significant number. The DPPH test results obtained the equation y = 0.3492x + 12,059, indicating that the antioxidant activity of suji extract of  is classified as moderate as the IC50 value of the extract of suji is108,65 ppm
The Comparison of IC50 of Suji (Pleomele angustifolia) which is Extracted by Different Solvent Bahri, Syamsul; Setiadi, Dadi; Lestari, Tri Ayu; Setiawan, Heru; Hakim, Muhammad Alfianul; Cahyadi, Lalu M. Zidan
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9559

Abstract

The Determination both of IC50 of Suji (Pleomele angustifolia) and its phytochemical subtances content which extracted by a different solvent has been conducted. The aim of this research is to determine kind of solvent which achieve better result. The method used is laboratory experiments for phytochemical screening and antioxidant activity assay. This research consists of 2 (two) stages. First stage aims to obtain extract of suji by using certain solvent, and the second stage is carried out to examine  secondary metabolite compounds of the extract using phytochemical screening and then to determine the antioxidant activity of extract using the DPPH method. This research used  2 kinds of solvent, ethanol plus ethyl acetate in the first sample, and the second one by ethanol only. Sample which extracted by ethanol plus ethyl acetate positively contains terpenoids, whereas flavonoid and  alkaloids are detected in a significant number. The DPPH test results obtained the equation y = 0.3492x + 12,059, indicating that the antioxidant activity of suji which extracted by ethanol plus ethyl acetate is classified as moderate as the IC50 value of the extract of suji is108,65 ppm. The other sample which extracted just by ethanol also contains terpenoid, flavonoid, and alkaloid, but in a greater number. In the second sample also detected steroid compound which undetectable in the first sample. DPPH score in the second sample which extracted  by ethanol. is y= 06352x + 18, 786. By using this equation we found 49.14 ppm as IC50 score. This score is classified as strong activity. This result shows that antioxidant activity of the second extract is stronger than the first one. The result also meaning that ethanol is more effective in extracting phytochemical compounds of suji than ethanol plus ethyl acetate.
Effect of Suji Leaf Ethanolic Extract (Dracaena angustifolia) on The Sperm Motility of Mice (Mus musculus) Exposed to Cigarette Smoke Hakim, Muhammad Alfianul; Bahri, Syamsul; Lestari, Tri Ayu
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.12078

Abstract

Cigarette smoke exposure can induce oxidative stress, leading to reduced sperm motility. Suji leaves contain antioxidants, including flavonoids, alkaloids, terpenoids, and steroids. This study aims to determine the effect of cigarette smoke exposure on mouse sperm motility and the effect of suji leaf ethanolic extract on the sperm motility of mice exposed to cigarette smoke. This study employed an experimental research design using a factorial Completely Randomized Design (CRD) involving 48 mice. Two factors were combined in the treatment groups: the duration of cigarette smoke exposure (0, 5, 15, and 30 minutes) and the dose of suji leaf ethanolic extract (0, 250, 500, and 750 ppm). Sperm motility was assessed using a hemocytometer under a light microscope at 400× magnification. Data were analyzed using a two-way analysis of variance. The results of statistical analysis indicated that cigarette smoke exposure had a significant effect on mouse sperm motility (0.000 < 0.05), and that suji leaf ethanolic extract significantly affected the sperm motility of mice exposed to cigarette smoke (0.036 < 0.05). These results indicate that cigarette smoke exposure can reduce sperm motility, and the administration of suji leaf ethanolic extract is able to increase and improve the sperm motility of mice caused by cigarette smoke exposure. Thus, suji leaf ethanolic extract can serve as a natural antioxidant to improve sperm motility affected by cigarette smoke exposure.
Protective Effect of Dracaena angustifolia Ethanolic Extract on Sperm Viability of Mice (Mus musculus) Bahri, Syamsul; Setiadi, Dadi; Lestari, Tri Ayu; Setiawan, Heru; Hakim, Muhammad Alfianul; Cahyadi, Lalu Zidan
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.12220

Abstract

An antioxidant is a substance that helps protect cells in the body from damage caused by unstable molecules called free radicals. Natural antioxidants are usually better for everyday nutrition and overall wellness. Natural antioxidants that come from plants, animals, or other natural sources are generally preferred because they come with other beneficial nutrients such as fiber, vitamins, and plant compounds. Plants contain relatively high levels of secondary metabolites such as flavonoids, phenolic acids, tocopherols, polyphenols, and tannins. One species of tropical plant that is rich in antioxidants is Dracaena angustifolia.  The research aims to study the capability of the ethanolic extract of Dracaena angustifolia in protecting sperm viability following cigarette exposure. Data analysis showed that tobacco smoke exposure significantly decreases sperm viability, but treatment with the ethanolic extract of Dracaena angustifolia significantly maintains sperm viability. It is concluded that the ethanolic extract of Dracaena angustifolia has potential in protecting normal sperm viability following smoking cigarette exposure.
Co-Authors Cahyadi, Lalu M. Zidan Cahyadi, Lalu Zidan Dadi Setiadi Heru Setiawan Lestari, Tri Ayu Syamsul Bahri