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All Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Surya
Safitri, Putri Gita Ayu
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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1’- Acetoxychavicol acetate Suppresses Osteosarcoma Cell Proliferation through the PI3K Pathway: A Molecular Docking and Cytotoxicity Study Safitri, Putri Gita Ayu; Da’i, Muhammad; Wulandari, Febri
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.640

Abstract

Background: This study aims to investigate anticancer properties of 1'-Acetoxychavicol acetate (ACA), a phenylpropanoid substance obtained from the rhizomes of the Alpinia genus, which has been extensively studied. However research on its cytotoxic effects, particularly against osteosarcoma cells, has never been donenot been conducted. The purpose of the research is to investigate the anticancer potential of ACA to support the its development as a novel therapeutic candidate.Materials and methods: This study assessed ACA’s initial anticancer potential through in vitro cytotoxic tests on normal human osteoblast cells (hFOB) and osteosarcoma cells (MG-63) using the MTT assay. Additionally, bioinformatics analyses, including target prediction, gene ontology, hub gene identification, protein-protein interactions (PPI) network construction, Kyoto encyclopedia of genes and genomes (KEGG) pathways analysis, disease association analysis, and molecular docking, were performed. Results: The cytotoxicity test on normal hFOB showed an IC50 of 45.05 µM, while in MG-63 osteosarcoma cells, the IC50 was 20.41 µM. In the bioinformatics test, top five target genes identified were SRC, GNAI1, PIK3CD, PIK3CB, PIK3R3. Molecular docking analysis showed that, the native PI3KD ligand showed a strong binding affinity of -10.99 kcal/mol and interacted with more amino acid residues.Conclusion: Overall, ACA exhibits promise as a treatment option to inhibit osteosarcoma cell proliferation by targeting the PI3K pathway. To develop ACA as a potential osteosarcoma therapeutic candidate, extensive in vitro research is needed.   Keywords: 1-Acetoxychavicol acetate, bioinformatics, cytotoxicity, osteosarcoma, PI3K pathway
Study of Formulation and Evaluation of the Potential Inhibitory Power of Basil Leaf Extract Hand Sanitizer Gel (Ocimum Sanctum L.) Against Staphylococcus Aureus: Literature Review Melinda, Nailus Amany; Safitri, Putri Gita Ayu; Laili, Fauza Nisfu; Himmah, Siti Ro'yatul
Jurnal Surya Vol 14 No 2 (2022): VOL 14 NO 02 AGUSTUS 2022
Publisher : Universitas Muhammadiyah Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38040/js.v14i2.481

Abstract

Introduction: The basil plant (Ocimum sanctum) has the potential to be developed into antibacterial due to the content of tannin compounds, flavonoids, and essential oils. Antibacterial is a compound produced by microorganisms so that it can inhibit the growth of bacteria and kill bacteria that cause infection. The number of side effects so that many antibacterial developments are carried out derived from natural ingredients. The development of formulas for hand sanitizer gel preparations has been widely developed, including with various studies using various concentrations of basil leaf extract. This study was conducted to determine the gel formula for hand sanitizer ethanol extract of basil leaves (Ocinum Sanctum L.) which has the best inhibition against Staphylococcus bacteria.Methods: Review literature is obtained from several journal libraries, journal searches, and trusted sites.Results: There are 14 journals as a library, which display results from antibacterial gel preparation formulations, bacterial inhibitory testing and evaluation testing of basil leaves. The results of the review showed that basil leaves (Ocimum sanctum L.) have antibacterial activity against Staphylococcus aureus bacteria.Conclusion: The best hand sanitizer gel formula was in a study conducted with a concentration of 8% basil leaf extract with an inhibitory power of 12.0 ± 3.10 mm which was included in the category of strong inhibitory power.
Co-Authors Da’i, Muhammad Himmah, Siti Ro'yatul Laili, Fauza Nisfu Melinda, Nailus Amany Wulandari, Febri