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All Journal Jurnal Biologi Tropis Jurnal Biosense
Afandi, Echa Azkia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Polyploid Induction of Long Bean (Vigna sinensis) Using Colchicine with Different Concentrations Fitri, Anisa; Afandi, Echa Azkia; Anggun, Septiasri; Yuni Ahda
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9063

Abstract

Long bean (Vigna sinensis) is a plant from the Leguminosae family, which has an important role in the Indonesian economy because it is widely consumed as food. The purpose of this research is to utilize mutation technology in supporting plant breeding to produce superior long bean varieties. The research method carried out was a non-factorial Randomized Group Design (RAK), namely soaking the seeds of long bean plants with colchicine to see the effect of colchicine treatment on chromosomal mutations in long bean plants. The results showed significant differences between colchicine concentrations of 0.02% and 0.03%. At a concentration of 0.02%, the cell nucleus appeared solid red and in the center of the cell, but the number and shape of chromosomes were not clearly visible. This concentration is not optimal for inducing polyploidy. Meanwhile, at a concentration of 0.03%, the chromosomes were more clearly visible than the previous concentration.
PENGARUH RADIASI SMARTPHONE DALAM KONDISI AIRPLANE MODE TERHADAP HISTOLOGI GINJAL MENCIT BETINA Ayuningtyas, Maulia Indah; Mellani Rachma; Fhuji Winardi; Afandi, Echa Azkia; Yusni Atifah; Dwi Hilda Putri
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6697

Abstract

The rapid advancement of technology and information has led to a significant increase in smartphone usage in Indonesia, reaching 67.88% in 2022. Smartphones emit non-ionizing electromagnetic waves that may pose biological effects, particularly on the kidneys, which play a crucial role in maintaining the body's homeostasis. Previous studies have reported that electromagnetic radiation can induce histological damage in the kidneys through oxidative stress mechanisms, including tubular narrowing, Bowman’s capsule dilation, and tubular epithelial necrosis. Airplane mode is known to significantly reduce electromagnetic radiation exposure. This study aimed to determine the effect of smartphone radiation in airplane mode on the kidney histology of female mice. An experimental design was employed using four female mice divided into one control group and three treatment groups, which were exposed to a smartphone in airplane mode for 4, 6, and 8 hours per day for 7 consecutive days. Kidney tissues were processed using paraffin embedding and stained with Hematoxylin–Eosin for microscopic examination. The results showed no significant histological differences between the control and treatment groups. The glomerulus, Bowman’s capsule space, and proximal and distal convoluted tubules appeared normal without signs of narrowing, dilation, hemorrhage, or necrosis. These findings indicate that smartphone radiation in airplane mode does not cause histological damage to the kidneys of female mice.
Co-Authors Anggun, Septiasri Atifah, Yusni Ayuningtyas, Maulia Indah Dwi Hilda Putri Fhuji Winardi Fitri, Anisa Mellani Rachma Yuni Ahda