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All Journal Journal of Applied Food Technology Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Prima Putra, Masagus Muhammad
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Earth & Planetary Sciences Education Engineering Other

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Influence of Enzyme Concentration, Hydrolysis Duration, and Drying Temperature on the Production of Antioxidant-containing Peptide from Catfish (Clarias sp.) Gills Prima Putra, Masagus Muhammad; Wisnumurti, Winnen; Athallah, Nezar Helmi; Zaen, Al Tarich Arasy; Sahura, Aulia Rachma; Ustadi, Ustadi
Journal of Applied Food Technology Vol 12, No 1 (2025)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.26160

Abstract

Catfish gills are a potential raw material for producing protein hydrolysate with antioxidant activity. Protein hydrolysate was produced using crude papain enzyme in two stages. The first stage was to obtain the optimum enzyme concentration (w/w) including 1%, 2%, 3%, 4%, 5% and 6%, with 0% as a control, followed by the second stage to obtain the optimum hydrolysis duration for 24, 48, 72, and 96 hours, with 0 hour as a control. The supernatant containing protein hydrolysate was then dried using an oven at various temperatures, including 60, 70, and 80°C, for 48 hours to obtain catfish gill protein hydrolysate (CGH) powder. CGH was tested for degree of hydrolysis (DH) and antioxidants, including DPPH and ABTS. The highest antioxidant activity was obtained from 3% papain with DPPH and ABTS values of 85% and 9.57 µM TEAC, respectively. Further stage on the hydrolysis duration gave 48 hours as the optimal one, with antioxidant activity of 85.25 % for DPPH and 4.29 µM TEAC for ABTS. The oven-drying temperature concluded that CGH has stable antioxidant activity. The IC50 on antioxidant activity based on DPPH ranged from 1799.85 mg/L to 1749.50 mg/L (IC50 of ascorbic acid was 2.61 mg/L) and was included as very weak. Even though based on IC50, CGH has low antioxidant activity, the protein content was found to be high (56.86±1.51%), which could be a high-protein food additive.
Investigation of anisakis infection and allergic potential on largehead hairtail (Trichiurus lepturus) from south coast of Yogyakarta Indonesia Prima Putra, Masagus Muhammad; Busyro, Ihda Khozainul; Puspita, Indun Dewi; Setyobudi, Eko
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.45035

Abstract

Anisakis spp. are parasites distributed worldwide that often infect marine fish species. This study investigated the prevalence, mean intensity, and Anisakis larvae species, and identified the allergenic potential of T. lepturus caught in the southern coasts of Yogyakarta, Indonesia. In total, 50 fish that were caught by the fishermen were measured for length and weight and examined for Anisakis larvae infection. The results showed that larvae were found in the abdominal cavity (87.03%), organs (7.95%), gonads (2.93%), and digestive systems (2.09%). The prevalence of Anisakis infection was high (64%), with a mean intensity of 7.47 larvae/fish. Molecular analysis revealed that the nematode was Anisakis typica. Therefore, it poses a potential health risk to humans. Consuming fish infected with Anisakis can cause allergic reactions even after the fish has been cooked. The identification was done through protein profile antigen detection of fish flesh, both infected and non-infected, using SDS-PAGE. In this study, the heating process was applied (60, 90, and 120) for 15 minutes. The result of allergenic potential detection from T. lepturus infected by Anisakis showed four protein bands correlated with Anisakis allergens Ani s 1 (24 kDa), Ani s 2 (97 kDa), Ani s 3 (41 kDa), and Ani s 7 (139 kDa) in all the infected fish, both fresh and processed.Keywords:AnisakisAllergenInfectionIntensityPrevalence
Investigation of anisakis infection and allergic potential on largehead hairtail (Trichiurus lepturus) from south coast of Yogyakarta Indonesia Prima Putra, Masagus Muhammad; Busyro, Ihda Khozainul; Puspita, Indun Dewi; Setyobudi, Eko
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.45035

Abstract

Anisakis spp. are parasites distributed worldwide that often infect marine fish species. This study investigated the prevalence, mean intensity, and Anisakis larvae species, and identified the allergenic potential of T. lepturus caught in the southern coasts of Yogyakarta, Indonesia. In total, 50 fish that were caught by the fishermen were measured for length and weight and examined for Anisakis larvae infection. The results showed that larvae were found in the abdominal cavity (87.03%), organs (7.95%), gonads (2.93%), and digestive systems (2.09%). The prevalence of Anisakis infection was high (64%), with a mean intensity of 7.47 larvae/fish. Molecular analysis revealed that the nematode was Anisakis typica. Therefore, it poses a potential health risk to humans. Consuming fish infected with Anisakis can cause allergic reactions even after the fish has been cooked. The identification was done through protein profile antigen detection of fish flesh, both infected and non-infected, using SDS-PAGE. In this study, the heating process was applied (60, 90, and 120) for 15 minutes. The result of allergenic potential detection from T. lepturus infected by Anisakis showed four protein bands correlated with Anisakis allergens Ani s 1 (24 kDa), Ani s 2 (97 kDa), Ani s 3 (41 kDa), and Ani s 7 (139 kDa) in all the infected fish, both fresh and processed.Keywords:AnisakisAllergenInfectionIntensityPrevalence
Co-Authors Athallah, Nezar Helmi Busyro, Ihda Khozainul Eko Setyobudi Puspita, Indun Dewi Sahura, Aulia Rachma Ustadi Ustadi Wisnumurti, Winnen Zaen, Al Tarich Arasy