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All Journal Journal of Tropical Crop Science Jurnal Biotek
Gustiningsih, Dini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Pengaruh Komposisi Media Dasar pada Kultur Embrio Kelapa Kopyor (Cocos Nucifera L. var.kopyor) Putri , Halida Adistya; Gustiningsih, Dini; Sholeh, M. Mahftuchin; Firdaus, Fahmi
Jurnal Biotek Vol 13 No 1 (2025): JUNI
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v13i1.55894

Abstract

 Kopyor coconut has the potential to be an export crop native to Indonesia. The low production of kopyor coconut in Indonesia occurs because kopyor coconut cannot germinate conventionally like an endosperm mutation. This is due to the food reserves in the form of fruit flesh in crumb condition (kopyor), so it cannot supply sufficient nutrients for the seed germination process. The only appropriate and effective germination method to obtain Kopyor coconut seeds is in vitro embryo culture. This study aimed to determine the composition of the best culture media types for the growth of Kopyor coconut embryos. The study was arranged using a non-factorial Completely Randomized Design with three levels of media types: M1: Y3 + BA 4 mg L-1. M2: De Fossard + BA 4 mg L-1, and M3: Modified Media (Macro and Micro: Eeuwens, Organic: De Fossard) + BA 4 mg L-1. The results of the study showed that in the germination phase, M1 media was an effective medium for the germination of coconut kopyor up to 16 MST, where there are embryos in phase 4/embryos that swell more (10 embryos) compared to M2 media (3 embryos) and M3 media (2 embryos). After evaluating the planting method, media composition, and replacement of ZPT, the dominant embryos experienced germination, where there were 30% of embryos in the modified media (M3) tended to be higher than other media treatments, but not significantly different
Response of Cowpea Growth, Yield and Organic Acid Secretion in Acidic Soil to Variability in Population and Minus One Element Fertilizer Test Gustiningsih, Dini; Purnamawati, Heni; Lubis, Iskandar; Syukur, Muhamad; Kondo, Tomohiro; Higuchi, Hirokazu
Journal of Tropical Crop Science Vol. 10 No. 03 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.03.196-204

Abstract

Cowpea is a legume with the potential to serve as an alternative food source to replace soybeans in Indonesia; soybeans are primarily used in traditional foods such as tempeh and tofu. Currently, 70% of Indonesia's soybean demand is met through imports. Cowpea exhibits the ability to thrive in suboptimal soils with low fertility levels. One of the major challenges in Indonesian agriculture is the prevalence of acidic soils, which are typically deficient in essential nutrients. Notably, a lack of phosphorus (P) is a significant limiting factor affecting crop productivity in these acidic soils. To address this issue, a study was conducted to investigate how cowpea can adapt and grow in acidic soil conditions. The first experiment aimed to understand the impact of plant density and fertilization methods on the growth and yield of cowpea. The results indicated that planting one cowpea plant per hole resulted in a higher number of leaves and branches. Additionally, the treatment with complete NPK showed a higher number of pods, although this was not significantly different from the treatment with reduced phosphorus (minus P). These findings suggest that cowpea can thrive in acidic soil even under conditions of phosphorus deficiency. Cowpea has developed an external adaptation mechanism through the secretion of organic acids from its roots to survive in low-phosphorus acidic soils. As a follow-up to these findings, a second experiment was conducted to identify the types of organic acids secreted by cowpea roots under conditions of phosphorus deficiency, using High-Performance Liquid Chromatography (HPLC). The results revealed that the organic acids produced by cowpea root exudates were primarily malic acid and oxalic acid. These research outcomes provide valuable information for growers, indicating that cowpea can be successfully cultivated in acidic soils. Cowpea's ability to produce organic acids allows it to thrive in such environments even when phosphorus levels are low.
Co-Authors Alrizal, Fahmi Firdaus Faqih Udin Higuchi, Hirokazu Iskandar Lubis Kondo, Tomohiro Muhamad Syukur Putri , Halida Adistya Sholeh, M. Mahftuchin