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All Journal Bioscientist : Jurnal Ilmiah Biologi Metamorfosa: Journal of Biological Science Open Access DRIVERset
Idramsa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Pengembangan E-LKPD Berbasis STEM yang Berorientasi pada Keterampilan Proses Sains dan Pemahaman Konsep Peserta Didik pada Materi Bioteknologi di SMA Swasta Shafiyyatul Amaliyyah Medan Sari, Siti; Idramsa; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17795

Abstract

This study aims to develop, validate, and examine the effectiveness of a STEM-based Biotechnology E-LKPD as a solution to the limitations of biology learning media, which are generally focused on printed texts and lecture methods. The research employed a Research and Development (R&D) approach using the 4D model (Define, Design, Develop, and Disseminate), involving expert validation, teacher responses, individual trials, and implementation in both experimental and control classes SMA Swasta Shafiyyatul Amaliyyah Medan. Validation results indicated that the product achieved very good criteria, with scores of 87.61% from material experts, 93.75% from learning and media experts, and 95.23% from language experts. Teacher responses to the E-LKPD reached 97.69% (very good), while students’ individual trial responses recorded 96.31% (very good). The implementation in the experimental class demonstrated a significant improvement in science process skills with an average percentage of 89.2%, compared to 61% in the control class. Furthermore, students’ conceptual understanding increased with an N-Gain score of 0.75 (high) in the experimental class, compared to 0.60 (medium) in the control class. These findings conclude that the STEM-based Biotechnology E-LKPD is valid, practical, and effective in enhancing students’ science process skills and conceptual understanding, aligning with the goals of the Merdeka Curriculum.
Isolasi dan Karakterisasi Bakteri Kitinolitik Endofit dari Akar Cabai (Capsicum Annuum L.) serta Potensinya dalam Menghambat Fusarium oxysporum Gultom, Ade Yuni Kartika; Idramsa; Pane, Wahdini; Pangaribuan, Joice; Nasution, Annisa Sepriani; Panjaitan, Josiva Risromanna; Panjaitan, Santi Grace
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 2 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i02.p6

Abstract

INTISARI Cabai merah (Capsicum anuum L.) merupakan tanaman hortikultura yang termasuk dalam famili Solanaceae dan banyak dimanfaatkan sehingga bernilai jual tinggi. Namun produksi masih rendah salah satunya disebabkan keberadaan jamur patogen Fusarium oxyporum. Teknik untuk mengatasi permasalahan ini yaitu dengan penggunaan aktivitas enzim kitinase bakteri kitinolitik endofit. Adapun tujuan dari penelitian ini yaitu mengisolasi dan karakterisasi bakteri kitinolitik endofit dari akar cabai yang berpotensi sebagai agen pengendali hayati. Desain penelitian merupakan deskriptif kualitatif dengan melakukan isolasi bakteri dan karakterisasi secara makrosokpik, dan secara fenotipik dengan pengujian biokimia serta genotipik dengan metode utama yaitu amplifikasi DNA menggunakan Polymerase Chain Reaction (PCR), sekuensing gen 16s rRNA, analisis BLAST berdasarkan NCBI, serta analisis filogenetik menggunakan MEGA XI. Selain itu, melakukan perhitungan indeks kitinolitik bakteri di media koloidal kitin serta persentase zona hambat yang terbentuk pada uji antagonis secara dualculture. Hasil penelitian ini menunjukkan isolat CAL1 yang memiliki nilai indeks kitinolitik tertinggi yaitu 0,24 diikuti dengan isolat CAL2 yang memiliki nilai indeks kitinolitik yaitu 0,20, dan CAL1 memiliki persentase daya hambat tertinggi terhadap jamur yaitu sebesar 35% (skala 2) serta diikuti oleh isolat CAL2 memiliki persentase daya hambat terhadap jamur yaitu sebesar 17% (skala 1). Isolat CAL1 dilanjutkan dengan karakterisasi hingga tahap spesies dan merupakan bakteri Bacillus cereus dengan kemiripan 99.65% dengan bootstrap sebesar 97%. Kata kunci: Bakteri kitinolitik endofit, Capsicum annuum, Bacillus cereus, Fusarium oxysporum, Gen 16s rRNA
Isolasi dan Karakterisasi Bakteri Kitinolitik Endofit dari Akar Cabai (Capsicum Annuum L.) serta Potensinya dalam Menghambat Fusarium oxysporum Gultom, Ade Yuni Kartika; Idramsa; Pane, Wahdini; Pangaribuan, Joice; Nasution, Annisa Sepriani; Panjaitan, Josiva Risromanna; Panjaitan, Santi Grace
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 2 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i02.p6

Abstract

INTISARI Cabai merah (Capsicum anuum L.) merupakan tanaman hortikultura yang termasuk dalam famili Solanaceae dan banyak dimanfaatkan sehingga bernilai jual tinggi. Namun produksi masih rendah salah satunya disebabkan keberadaan jamur patogen Fusarium oxyporum. Teknik untuk mengatasi permasalahan ini yaitu dengan penggunaan aktivitas enzim kitinase bakteri kitinolitik endofit. Adapun tujuan dari penelitian ini yaitu mengisolasi dan karakterisasi bakteri kitinolitik endofit dari akar cabai yang berpotensi sebagai agen pengendali hayati. Desain penelitian merupakan deskriptif kualitatif dengan melakukan isolasi bakteri dan karakterisasi secara makrosokpik, dan secara fenotipik dengan pengujian biokimia serta genotipik dengan metode utama yaitu amplifikasi DNA menggunakan Polymerase Chain Reaction (PCR), sekuensing gen 16s rRNA, analisis BLAST berdasarkan NCBI, serta analisis filogenetik menggunakan MEGA XI. Selain itu, melakukan perhitungan indeks kitinolitik bakteri di media koloidal kitin serta persentase zona hambat yang terbentuk pada uji antagonis secara dualculture. Hasil penelitian ini menunjukkan isolat CAL1 yang memiliki nilai indeks kitinolitik tertinggi yaitu 0,24 diikuti dengan isolat CAL2 yang memiliki nilai indeks kitinolitik yaitu 0,20, dan CAL1 memiliki persentase daya hambat tertinggi terhadap jamur yaitu sebesar 35% (skala 2) serta diikuti oleh isolat CAL2 memiliki persentase daya hambat terhadap jamur yaitu sebesar 17% (skala 1). Isolat CAL1 dilanjutkan dengan karakterisasi hingga tahap spesies dan merupakan bakteri Bacillus cereus dengan kemiripan 99.65% dengan bootstrap sebesar 97%. Kata kunci: Bakteri kitinolitik endofit, Capsicum annuum, Bacillus cereus, Fusarium oxysporum, Gen 16s rRNA
Uji Aktivitas Klorofil Daun Putri Malu (Mimosa pudica Linn) Sebagai Antibakteri Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Rahmah, Annisa; Idramsa; Ritonga, Iin Agustina
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18470

Abstract

Infectious diseases caused by pathogenic bacteria such as Staphylococcus aureus and Escherichia coli remain a health problem, especially due to increasing antibiotic resistance. Therefore, antibacterial alternatives from natural materials are needed, one of which is the chlorophyll pigment from Mimosa pudica Linn. This study aims to test the antibacterial activity of chlorophyll from young and old leaves of M. pudica Linn and compare the inhibitory power of both against test bacteria. This study is an experimental study that includes the preparation of simple drugs, ethanol extraction, liquid-liquid fractionation, purification using thin layer chromatography (TLC) and column chromatography, and antibacterial testing by disc diffusion method using standard bacterial inoculum McFarland 0.5. The purification results showed an Rf value of 0.87 with one spot indicating pure chlorophyll fraction. The antibacterial activity of chlorophyll is relatively weak, but the chlorophyll fraction of old leaves showed greater inhibitory power than young leaves. The highest inhibition zone against S. aureus was obtained from old leaf chlorophyll of 4.00 ± 1.01 mm, while against E. coli it was 2.54 ± 0.52 mm.
Pengembangan E-Modul Berbasis SETS (Science, Environment, Technology, and Society) Pada Materi Inovasi Teknologi Biologi untuk Meningkatkan Kemampuan Berpikir Kritis Siswa Kelas X SMA/MA Zahrah, Thifal; Idramsa; Lubis, Khairiza
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19381

Abstract

This study aims to describe the validity, teacher responses, student responses, practicality, effectiveness, and the improvement of critical thinking skills through the development of a SETS-based. This study employed a Research and Development (R&D) approach using the 4D model. The results show that: (1) The validity of the SETS-based e-module is categorized as very feasible, with an average score of 85% for material validation, 95% for media validation, and 89% for language validation, indicating that the e-module is suitable for instructional use. (2) Teacher responses indicate that the SETS-based e-module helps students develop more comprehensive and contextual thinking, the average score obtained for the model design aspect was 85%, the concept mastery aspect was 90% and the learning motivation aspect was 88%. (3) Student responses demonstrate that the e-module meets the criteria of practicality, comfort, and usefulness, and successfully enhances learning interest and motivation. The results obtained for the module design aspect were 95%, the concept mastery aspect was 89% and the learning motivation aspect was 90%. (4) The practicality and effectiveness tests reveal an average score of 91%, placing the e-module in the highly practical and highly effective categories for learning. (5) The improvement of students’ critical thinking skills is evidenced by the t-test 0.05, which indicates a significant difference between pretest and posttest scores. The average N-Gain score of 0.758, categorized as high, shows that the SETS-based e-module increased students’ critical thinking ability by 76%. Overall, the developed SETS-based e-module is valid, practical, effective, and capable of significantly improving students’ critical thinking skills.
Co-Authors Ashar Hasairin Gultom, Ade Yuni Kartika Lubis, Khairiza Nasution, Annisa Sepriani Pane, Wahdini Pangaribuan, Joice Panjaitan, Josiva Risromanna Panjaitan, Santi Grace Rahmah, Annisa Ritonga, Iin Agustina Sari, Siti Zahrah, Thifal