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All Journal BIOVALENTIA: Biological Research Journal Journal of Soilscape and Agriculture
Ahmad Iqbal Rananda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Porang (Amorphophallus muelleri B.) Indirect Organogenesis Nur Khozin, Mohammad; Ahmad Iqbal Rananda; Didik , Pudji Restanto
BIOVALENTIA: Biological Research Journal Vol. 10 No. 2 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.2.2024.334

Abstract

Organogenesis is regeneration mechanism on the process of organ formation derived from somatic or non-meristematic tissue on in vitro technique. Organogenesis can occur directly or indirectly. This research used porang leaves as explant, and medium MS Basal with addition such as sucrose, agar, combination of PGR’s 2,4 D and NAA, and aquadest. This research method used the Completely Randomize Design (CRD) with 9 different level combination of 2,4 D and NAA such as 2,0 ppm 2,4 D + 1,5 ppm NAA (A1), 2,0 ppm 2,4 D + 2,0 ppm NAA (A2), 2,0 ppm 2,4 D + 2,5 ppm NAA (A3), 2,5 ppm 2,4 D + 1,5 ppm NAA (A4), 2,5 ppm 2,4 D + 2,0 ppm NAA (A5), 2,5 ppm 2,4 D + 2,5 ppm NAA (A6), 3,0 ppm 2,4 D + 1,5 ppm NAA (A7), 3,0 ppm + 2,0 ppm NAA (A8), 3,0 ppm 2,4 D + 2,5 ppm NAA (A9) with 3 repetitions for each combination. Quantitative data such as the percentage of explants that appeared callus and roots were tested statistically by using ANOVA to determine the significance of the effect of the treatment. Treatments that had significant results were then tested using the DMRT test with a level of α=5%. Qualitative data were observed visually and analyzed descriptively. Combination concentration treatment of 2,4 D and NAA have the significant effect on callus and root formation. The level of 2 ppm 2,4 D and 2 ppm NAA is the best treatment on callus formation process in terms of 100% callus emerge and the early day appears at 24 days after planting and the best treatment on the process of root formation in terms of percentage roots emerge 100% and the early day roots appears at 46 days after planting. Indirect organogenesis is type of of root regeneration.
The Effect of Different Sterilization Methods on Obtaining Sterile Leaf Explants of Porang (Amorphophallus muelleri B.) Ahmad Iqbal Rananda; Nur Khozin, Mohammad
Journal of Soilscape and Agriculture Vol. 2 No. 1 (2023): September 2023
Publisher : University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jsa.v2i1.142

Abstract

Porang (Amorphophallus muelleri B.) is a type of tuber that contains glucomannan, which is used as a raw materials for the food, chemical, pharmaceutical and cosmetic industries. In vitro culture as porang propagation method using plant parts (leaf explants) was developed under aseptic environmental conditions. Sterilization is an important aspect in the success of micropropagation in obtaining sterile explants. This research method used a Completely Randomized Design (CRD) with 2 factors, namely clothes bleach sterilant containing NaOCl 5.25% (Bayclin®) at 30% concentration for 15 minutes (Treatment A), and using clothes bleach containing NaOCl 5.25% (Bayclin®) at 30% concenteration for 15 minutes + 70% alcohol for 15 seconds (Treatment B). Each treatment was filled with 3 explants, and every treatment was repeated 27 times, so that experimental units were obtained with a total of 162 explants. Treatment B is the best treatment in obtaining sterile explants, because it is more effective in removing contamination and inhibiting contamination and browning with survival the highest rate of 51.85% and the lowest mortality rate of 48.15%, it can also suppress and inhibit the appearance of contamination and browning compared to treatment A.
Co-Authors Didik , Pudji Restanto Nur Khozin, Mohammad