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Potensi Fungi Endofit dari Daun Sirih (Piper Betle L.) sebagai Alternative Pencarian Senyawa Antibiotik terhadap Bakteri Penyebab Infeksi Marhamah Marhamah; Muh Danial Fajri; Lola Tulak Rerung
JURNAL RISET RUMPUN ILMU KESEHATAN Vol. 4 No. 2 (2025): Agustus : Jurnal Riset Rumpun Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrikes.v4i2.5718

Abstract

Infections and antibiotic resistance pose significant challenges to global healthcare. This study aims to evaluate the antibacterial potential of endophytic fungi isolated from betel leaves (Piper betle L.) against Staphylococcus aureus and Staphylococcus epidermidis. The research involved isolating endophytic fungi using Potato Dextrose Agar (PDA), purifying the isolates, and testing their antibacterial activity through the disk diffusion method. The results revealed that three endophytic fungal isolates produced inhibition zones, with the highest average diameter of 17.46 mm against Staphylococcus aureus and 18.66 mm against Staphylococcus epidermidis. These findings indicate the potential of betel leaf endophytic fungi as a natural source of antibacterial agents. This study underscores the role of endophytic fungi in developing new antibiotics to combat bacterial resistance.
Potensi Fungi Endofit Daun Sambung Nyawa (Gynura Procumbens) sebagai Alternatif Antibakteri Baru Muh Danial Fajri; Djulfikri Mewar; Marhamah Marhamah
JURNAL RISET RUMPUN ILMU KESEHATAN Vol. 4 No. 2 (2025): Agustus : Jurnal Riset Rumpun Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrikes.v4i2.5804

Abstract

The Sambung Nyawa plant (Gynura procumbens) is known to have various benefits, including as an antibacterial. This study aims to identify and evaluate the antibacterial properties of secondary metabolites from endophytic fungi isolated from Sambung Nyawa leaves against Staphylococcus epidermidis and Staphylococcus aureus bacteria. Fungi were isolated and then fermented for 7 days using Nutrient Broth media. Antibacterial activity was evaluated using agar diffusion technique, with zone of inhibition as an indicator of activity. The results showed that IFDSN 2 fungal isolate had the highest zone of inhibition, with a very strong category against S. aureus and strong against S. epidermidis. This finding supports the potential of endophytic fungi as an environmentally friendly natural antibacterial source.
Manis Belum Tentu Aman: Edukasi Pemanis Buatan pada Minuman Kemasan bagi Siswa SMP IT Citra Ummat Kota Tidore Kepulauan Muhammad Subhan A. Sibadu; Muthmainnah Muthmainnah; Muh Danial Fajri; Amalia S. J. Kahar; Nur Naila; Dilla Putri Hi. Rajab; Humaira Salsabila; Muhammad Fauzy Andi Mattara
Nusantara: Jurnal Pengabdian kepada Masyarakat Vol. 6 No. 3 (2026): Agustus: NUSANTARA Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/nusantara.v6i3.10201

Abstract

The consumption of sugar-sweetened packaged beverages among adolescents continues to increase along with changes in lifestyle and dietary patterns. Limited knowledge regarding artificial sweeteners, recommended intake limits, and the potential health risks of excessive consumption may contribute to unhealthy beverage choices. This community service program aimed to improve students' knowledge of artificial sweeteners in packaged beverages through health education at SMP IT Citra Ummat, Tidore Islands City. The program involved 67 seventh- and eighth-grade students and was implemented through interactive lectures, discussions, question-and-answer sessions, supported by PowerPoint presentations, educational banners, and leaflets. Knowledge was assessed using pre-test and post-test questionnaires, and the data were analyzed using the Wilcoxon Signed Rank Test. The results showed that 33 students (49.3%) experienced increased knowledge scores, 23 students (34.3%) showed no change, and 11 students (16.4%) had decreased scores. Statistical analysis demonstrated a significant difference between pre-test and post-test scores (Z = -3.500; p = 0.000), indicating that the educational intervention effectively improved students' knowledge regarding artificial sweeteners in packaged beverages. The program also enhanced students' awareness of the importance of reading nutrition facts and ingredient labels before purchasing packaged beverages. Health education can therefore serve as an effective promotive strategy to improve food safety literacy among adolescents.