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All Journal Jurnal Farmasi
Frasesa, Roscha Ulfa
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Analysis of Flavonoid, Phenolic Content, and Antioxidant Activity in Chili Pepper (Capsicum frutescens L.) Fruit Stem Extract Fadhli, Haiyul; Putra, Diova Yuswidia; Trihardiyanto, Agus; Frasesa, Roscha Ulfa
Jurnal Farmasi (Journal of Pharmacy) Vol 14, No 1 (2025)
Publisher : SEKOLAH TINGGI ILMU KESEHATAN NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37013/jf.v14i1.360

Abstract

Cayenne pepper (Capsicum frutescens L.) is known to have many medicinal properties, especially its fruit. However, cayenne pepper fruit stalks have not been studied in depth and are only considered waste. This study aimed to ascertain the total flavonoid concentration, phenolic content, and antioxidant activity of the extract derived from cayenne pepper fruit. Sample extraction using multistage maceration with the help of sonication. Using 3 different solvents, namely n-hexane, ethyl acetate, and ethanol in a ratio of 1:4 (b/v), and then sonicated (30 minutes, 20-25 °C, 42 kHz). The calorimetric technique was used to determine the total flavonoid content (KTF). The Folin-Ciocalteu method was used to determine the total phenolic content (KTFe). And the DPPH method with a microplate reader was used to determine the antioxidant activity of ethanol extract. Total flavonoid content of 30.333 ± 1.32 mgQE/g extract, total phenolic content of 16.333 ± 0.89 mgGAE/g extract, and IC50 of 544.82 ± 0.01 µg/mL showed that ethanol extract of chili fruit stalk had weak antioxidant activity.
Co-Authors Haiyul Fadhli Putra, Diova Yuswidia Trihardiyanto, Agus