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All Journal Molekul: Jurnal Ilmiah Kimia
Azahra, Silena
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Kojic Acid from the Ethyl Acetate Fraction of Terminalia catappa Linn Fruit Flesh and its anti-cancer activity against HeLa cells Chasani, Moch.; Widyaningsih, Senny; Rastuti, Undri; Hanafi, Muhammad; Fauzi, Kevin Azhar; Azahra, Silena
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13579

Abstract

ABSTRACT. The ethyl acetate fraction of Terminalia catappa Linn. fruit flesh has anti-cancer activity against HeLa cells, with IC50 value is =165.37 ppm. The aim of this research is to isolate active anticancer compound from the ethyl acetate fraction of T. catappa fruit flesh. Anti-cancer active compounds from the ethyl acetate fraction of T. catappa fruit flesh were purified using column chromatography and continued with recrystallization. The compound isolate from the ethyl acetate fraction is clear yellowish crystals and has a melting point 150 ± 2 oC, has anti-cancer activity against HeLa cells of IC50 =413.695 ppm. Results of molecular structure identification using UV spectroscopy; FTIR; 1HNMR, 13CNMR and LC-MS are thought to be the compound 5-Hydroxy-2-(hydroxymethyl)-4H-pyran-4- one (Kojic acid) with a molecular weight of 142.36 gram/mol. The Kojic acid content in T. catappa Linn fruit flesh was found to provide information on the potential use of T. catappa fruit flesh extract in the pharmaceutical and cosmetics industries. Keywords: Anticancer activity, HeLa cells, Kojic acid, T. catappa Linn.
Antioxidant Activity of Flavonoids from T. catappa Linn Fruit Flesh Fractionated using Continuous Column chromatography Widyaningsih, Senny; Chasani, Moch.; Rastuti, Undri; Nurhandayani, Santi; Azahra, Silena; Pratiwi, Salma Yuni
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.17276

Abstract

ABSTRACT. Terminalia catappa L. is traditionally used as antioxidant anti-inflammatory, antimicrobial, hepatoprotector, vermifuge, anticancer and antidiabetic. Especially the flesh of T. catappa fruit has potential as an antioxidant. There has been no research that conducts a fractionation process using column chromatography to find antioxidant active fractions from fruit flesh of T. catappa. Therefore, this research aims to find the antioxidant active fraction of the fruit flesh T. catappa. The research steps began with the extraction process using methanol and continued with fractionation using n-hexane and ethyl acetate. The ethyl acetate fraction (with antioxidant activity IC50 = 246.3 ppm) was subjected to the first column chromatography with a gradient of increasingly polar eluents. The fractionation results obtained 4 subfractions with the highest antioxidant activity found in subfraction 3 (FG3) with IC50 = 81.9 ppm containing phenolic, flavonoids, and terpenoids compounds. FG3 was fractionated again using second column chromatography. The fractionation results obtained 2 subfractions with antioxidant activity IC50 = 76.936 ppm (FG1P) and 82.745 ppm (FG2P). FG1P contains phenolic secondary metabolites, namely flavonoids of the isoflavone class. The results provide information that the compounds acting as antioxidants in T. catappa fruit flesh are flavonoids presumably from the isoflavone class. The discovery of flavonoid compounds that act as antioxidants in T. catappa fruit flesh can be developed in the pharmaceutical industry. Keywords: Antioxidant, fruit flesh, Lisoflavone, T. catappa
Co-Authors Fauzi, Kevin Azhar Moch. Chasani Muhammad Hanafi Nurhandayani, Santi Pratiwi, Salma Yuni Senny Widyaningsih Undri Rastuti