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The Effect Nitrogen Fertilizer Application on the Resistance of Tobacco (Nicotiana tabacum L. ‘Bligon’) against Drought Stress Rima Vegi Santika; Siti Nurbaiti; Putra, Sidiq Permana; Rachmawati, Diah
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.09

Abstract

Tobacco (Nicotiana tabacum L.) is a plant that has high economic value. Water availability is a challenge in cultivating tobacco plants. Drought can affect plant physiology, reducing the metabolic rate and productivity of tobacco plants. Urea fertilizer contains nitrogen as a macronutrient, which plays a role in vegetative growth and water absorption in drought stress. This research aims to study the resistance response of tobacco to varying conditions of water availability and nitrogen fertilizer application. The water availability treatment provided includes 100%, 75% and 50% of the field capacity. Variations in the dose of nitrogen fertilizer were 0.6 g; 1.2 g; and 1.8 g/polybag. This research was conducted using a Randomized Complete Block Design (RCBD) with three replications. The parameters measured were resistance responses including H?O? content and antioxidant enzyme activity such as SOD, CAT, and APX. The results showed that the more severe the drought, the higher the H?O?  levels. The plant resistance mechanism is indicated by the activity of the antioxidant enzymes SOD, CAT, and APX, despite the fluctuating values observed during the experimental period. The application of nitrogen fertilizer at doses of 0.6 g, 1.2 g, and 1.8 g/polybag showed no significant differences in the physiological responses of the plants. As a result, a dose of 0.6 g/polybag can be recommended as an agronomically and economically efficient.
Calcium Silicate Application Enhances Oxidative Defense and Improves the Physiological and Growth Responses of Shallot (Allium cepa L. Aggregatum Group) Under Salinity Stress Putra, Sidiq Permana; Sukirno, Sukirno; Nugroho, Laurentius Hartanto; Rachmawati, Diah
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108192

Abstract

Indonesia’s shallot production still falls short of domestic demand, necessitating imports. Expanding cultivation into marginal coastal areas such as Yogyakarta is promising but constrained by soil salinity. Silicon can help by enhancing plant resistance to such abiotic stress. This study evaluated the physiological and biochemical responses of shallot plants (Allium cepa L. Aggregatum group) to the application of calcium silicate (CaSiO3) under saline conditions. The experiment employed a completely randomized design with 2 factors: CaSiO3 (0, 2, and 4 mM) and salinity (0, 2, 4, and 8 dS m⁻¹), each with 5 replications. Physiological parameters, antioxidant activity, and yield traits were analyzed using analysis of variance (ANOVA) followed by Duncan multiple range test (DMRT) at p ≤ 0.05. CaSiO3 significantly alleviated salt stress by enhancing superoxide dismutase (SOD) activity and membrane stability, improving photosynthetic efficiency, promoting growth, and yield components. Under high salinity, 4 mM CaSiO3 reduced proline and H₂O₂ accumulation compared with untreated plants. These findings indicate that applying 4 mM CaSiO3 can enhance shallot productivity and resilience in saline coastal soils, supporting sustainable shallot self-sufficiency in Indonesia.