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All Journal Indonesian Journal of Chemical Science
apt. Eko Mugiyanto, Eko
Unknown Affiliation
Chemistry Physics

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Validation of Methods and Determination of Curcumin Levels in Carrying Herbal Medicine of Turmeric Tamarind Using UV-Vis Spectrophotometer Arum Dwi Nanda, Arum; Khusna Santika Rahmasari, Khusna; Achmad Vandian Nur, Vandian; apt. Eko Mugiyanto, Eko
Indonesian Journal of Chemical Science Vol. 14 No. 2 (2025): Indonesian Journal of Chemical Science
Publisher : Prodi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v14i2.14192

Abstract

Turmeric contains curcumin which works to increase the body's defense system against viruses or bacteria. The study aims to determine the validity parameters in the form of linearity, precision, accuracy, LOD, LOQ and determine the curcumin levels in carrying herbal medicine of turmeric tamarind. Method validation aims to ensure that the method to be used meets the requirements for the analytical application and can be justified for its correctness. The method was quantitative one using UV-Vis spectrophotometer. The results showed that the method met the linearity requirements with r: 0.9995, accuracy with a recovery value of 99.43%, precision with an RSD value of 0.49%, and LOD and LOQ with values of 0.0054 µg/mL and 0.0163 µg/mL. The curcumin content in the herbal medicine of turmeric tamarind sold at Ulujami Market, Pemalang in herbal medicine sample A was 0.09% and herbal medicine sample B was 0.07%.  
Co-Authors Achmad Vandian Nur, Achmad Vandian Arum Dwi Nanda, Arum Khusna Santika Rahmasari