Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal KNOWLEDGE: Jurnal Inovasi Hasil Penelitian dan Pengembangan Global Education Journal
Batafor, Yosephina M.J.
Unknown Affiliation
Arts Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Physics Social Sciences Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
SITOKSISITAS DAN IDENTIFIKASI SENYAWA AKTIF PADA IKAN BUNTAL (Tetraodon lunaris) DI PERAIRAN TIWATOBI Derosari, Bernadete Dian; Tukan, Maria Magdalena N. M.; Batafor, Yosephina M.J.
KNOWLEDGE: Jurnal Inovasi Hasil Penelitian dan Pengembangan Vol. 5 No. 3 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia (P4I)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/knowledge.v5i3.7019

Abstract

Pufferfish are a species known for their toxic content, tetraodotoxin (TTX). Tetrodotoxin levels in pufferfish are influenced by their habitat and diet. This study aimed to analyze the cytotoxicity and identify active compounds in pufferfish in the waters of Tiwatobi Village. This research began with sampling in the waters of Tiwatobi Village, then the samples were prepared in the Fisheries Product Technology processing room, Larantuka Teacher Training and Technology Institute. The samples were extracted with 75% ethanol using the maceration method. The extracted samples were analyzed for cytotoxicity using the Brine Shrimp Lethality Test (BSLT) method. Furthermore, the active compounds in the pufferfish ethanol extract were identified using GC-MS. The results of the study showed the cytotoxicity of pufferfish extract, expressed as an LC50 value of 49.479 ppm, which is categorized as moderately toxic. Compound analysis using GC-MS revealed 10 active compounds, consisting of: Ethyl Acetate, Di-n-propyl ether, Propane, 1-ethoxy-2-methyl, Propanoic acid- ethyl ester, Disulfide- dimethyl, Dimethyl Sulfoxide, S-Methyl methanethiosulfinate, Cyclotetrasiloxane- octamethyl, S-Methyl methanethiosulphonate dan Cyclopentasiloxane, decamethyl-. ABSTRAKIkan buntal merupakan spesies ikan yang terkenal dengan kandungan racunnya yaitu tetraodotoksin (TTX). kandungan tetrodotoksin yang terdapat pada ikan buntal dipengaruhi oleh hábitat dan makanannya. Penelitian ini bertujuan untuk menganalisis sitotoksisitas dan itentifikasi senyawa aktif pada ikan buntal di perairan Desa Tiwatobi. Penelitian ini diawali dengan pengambilan sampel diperairan Desa Tiwatobi, selanjutnya sampel di preparasi di ruang pengolahan Teknologi Hasil Perikanan, Institut Keguruan dan Teknologi Larantuka. Sampel di ekstraksi dengan etanol 75% menggunakan métode maserasi. Sampel hasil ekstraksi di análisis sitotoksisitasnya dengan métode Brine Shrimp Lethality Test (BSLT). Selanjutnya identifikasi senyawa aktif pada ekstrak etanol ikan buntal menggunakan GC-MS. Hasil penelitian menunjukan sitotoksisisitas ekstrak ikan buntal yang dinyatakan dengan nilai LC50 yaitu sebesar 49,479 ppm, yang masuk dalam kategori cukup toksik. Data hasil analisi senyaw menggunakan GC-MS ditemukan 10 senyawa aktif yang terdiri dari: Ethyl Acetate, Di-n-propyl ether, Propane, 1-ethoxy-2-methyl, Propanoic acid- ethyl ester, Disulfide- dimethyl, Dimethyl Sulfoxide, S-Methyl methanethiosulfinate, Cyclotetrasiloxane- octamethyl, S-Methyl methanethiosulphonate dan  Cyclopentasiloxane, decamethyl-.
Identification of Bacteria in Yellowfin Tuna (Thunnus albacares) With the Addition of Salt Lewar, Yulius Frederik; Nawastuti, Dati; Batafor, Yosephina M.J.
Global Education Journal Vol. 3 No. 1 (2025): Global Education Journal (GEJ)
Publisher : Civiliza Publishing, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59525/gej.v3i1.795

Abstract

One of the processed fishery products is se'i fish. Se'i is smoked meat processed traditionally using a smoking method with firewood and kosambi leaves. This study aims to identify the presence of Salmonella sp. and Escherichia coli bacteria in se'i tuna (Thunnus albacares) with salt treatment, as well as to evaluate factors that influence product safety. Tuna se'i samples were treated with coarse and fine salt additions with variations of 5 g and 10 g, as well as a control without salt. Bacterial identification was carried out using standard microbiological methods. The results showed that all samples, both control and salt treatment, were negative for Salmonella sp. and E. coli. This indicates that salt variation did not affect the results because all samples did not contain these bacteria. The dominant influencing factor was the use of garlic as a seasoning that contains allicin compounds with antibacterial properties that can inhibit the growth of spoilage bacteria. In addition, the packaging aspect plays an important role in improving the safety, quality, and competitiveness of se'i tuna products because it is safe for consumption and has great potential as a traditional specialty product.
Co-Authors Derosari, Bernadete Dian Lewar, Yulius Frederik Nawastuti, Dati Tukan, Maria Magdalena N. M.