Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Healthy Tadulako Journal (Jurnal Kesehatan Tadulako)
Putra, Galih Abisatya Hartono
Unknown Affiliation
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Comparison of Antibacterial Activity Tests of 70% and 96% Ethanol Extract on Siam Sambas Orange Leaves (Citrus nobilis var. microcarpa) Zakiah, Mistika; Asseggaf, Syarifah Nurul Yanti Rizki Syahab; Ulfah, Ridha; Putri, Triyana Harlia; Hendrayana, Bimo; Putra, Galih Abisatya Hartono
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 11 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/htj.v11i3.1705

Abstract

Background: Antibiotic resistance against bacterial infections such as Escherichia coli and Staphylococcus aureus, prevalent in tropical regions, necessitates alternative treatments. One potential solution is utilizing secondary metabolites found in Sambas Siamese orange leaves. These compounds may serve as antibacterial agents. Objective: This study aims to compare the effectiveness of 70% and 96% ethanol as solvents for extracting secondary metabolites from Sambas Siamese orange leaves. Methods: Extraction was performed using two types of solvents (70% and 96% ethanol) to obtain secondary metabolites. Antibacterial activity was tested using the disc diffusion method (Kirby-Bauer), and data were analyzed with One-Way Analysis of Variance (ANOVA). Results: Extraction with 96% ethanol yielded flavonoids (+++), terpenoids (++), steroids (++), and phenols (++). Meanwhile, extraction with 70% ethanol produced Mayer alkaloids (++), flavonoids (++), saponins (+), steroids (+++), and phenols (+++). The 96% ethanol solvent was more effective in extracting limonene compounds from the leaves. Conclusion: The 96% ethanol solvent demonstrated higher effectiveness compared to 70% ethanol in extracting secondary metabolites, particularly limonene, which has potential antibacterial properties, from Sambas Siamese orange leaves
Co-Authors Asseggaf, Syarifah Nurul Yanti Rizki Syahab Hendrayana, Bimo Mistika Zakiah Ridha Ulfah Triyana Harlia Putri