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All Journal Innovative: Journal Of Social Science Research
Tirtawati, Ni Putu Ayu
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Humanities Computer Science & IT Law, Crime, Criminology & Criminal Justice Public Health Other

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Desain Primer Secara In Silico untuk Amplifikasi Gen Pre-S2 Virus Hepatitis B Tirtawati, Ni Putu Ayu; Yowani, Sagung Chandra
Innovative: Journal Of Social Science Research Vol. 5 No. 4 (2025): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v5i4.20964

Abstract

Hepatitis B is a serious infectious disease caused by the Hepatitis B virus (HBV), which can progress to cirrhosis and hepatocellular carcinoma. Early detection of HBV infection is crucial to prevent disease progression, but the Pre-S2 gene region as a diagnostic target remains understudied. This gene plays a crucial role in viral infectivity, replication, and immune response, and its mutations are known to contribute to latent infection and the pathogenesis of liver cancer. This study aimed to design in silico primer pairs specific to the HBV Pre-S2 gene using a bioinformatics approach. The design was performed using Primer3Plus based on the HBV genome sequence from GenBank (Accession Number AB246345.1), and evaluated through physicochemical parameters such as length, melting temperature (Tm), GC content, and potential for secondary structure formation. The best candidates were then tested for specificity using Primer-BLAST against various HBV isolates. The results showed that the forward primer pair 5’-TTCCTCTTCATCCTGCTGCT-3’ and reverse primer pair 5’-AGTTTCCGTCCGAAGGTTTT-3’ were able to amplify the Pre-S2 gene with a PCR product length of 193 bp and exhibited high specificity for the HBV genome. These findings demonstrate the effectiveness of the in silico approach in designing primers for molecular detection of the HBV Pre-S2 gene and its potential applications in diagnosis, mutation research, and the development of prevention strategies.
Co-Authors Sagun Chandra Yowani