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All Journal Jurnal Riset Kimia Sains Natural: Journal of Biology and Chemistry Journal Of Research and Education Chemistry (JREC)
Hillman, Prima Fitria
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education

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Harnessing Indigenous Fermentation: Probiotic Lactic Acid Bacteria from West Sumatera’s Dadih Hillman, Prima Fitria; Titisari, Geliz Luh; Syafrizayanti, Syafrizayanti
Jurnal Riset Kimia Vol. 16 No. 2 (2025): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v16i2.853

Abstract

The potential of lactic acid bacteria (LAB) isolated from dadih, a traditional fermented buffalo milk from West Sumatra, was explored as functional starter cultures for yogurt production. The use of local LAB is considered essential due to Indonesia’s dependence on imported cultures, which often exhibit inconsistent performance and suboptimal quality. This study selected bacterial isolates based on their ability to grow in highly acidic media and their tolerance to bile salt treatment. Molecular identification was conducted by amplifying the 16S rRNA gene using the PCR method. Specific LAB isolates with high probiotic potential were further characterized based on their tolerance to acid and bile salts. The results showed that several LAB strains from dadih, such as Lactococcus lactis, Weissella paramesenteroides, and Weissella sp., exhibited good probiotic properties. These isolates could effectively ferment milk and produce yogurt with organoleptic qualities comparable to commercial cultures. In addition, Serratia marcescens, known as an opportunistic pathogen, was also detected during the isolation process; some of its strains are known to participate in fermentation processes. These findings support the utilization of local LAB as an alternative to imported cultures, promoting local food production, improving the nutritional value of fermented dairy products, and preserving traditional culinary heritage.
Pengaruh Suhu dalam Pembuatan Fasa Diam Silika Hibrid Monolit sebagai Studi Awal untuk Kromatografi Cair Sistem Kapiler Furqani, Firda; Bella, Ilona; Hillman, Prima Fitria; Lim, Lee Wah
Journal of Research and Education Chemistry Vol. 7 No. 2 (2025): Oktober
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/jrec.2025.vol7(2).25260

Abstract

Penelitian ini merupakan studi awal mengenai pembuatan dan karakterisasi fasa diam silika hibrid monolit untuk aplikasi pada kromatografi cair sistem kapiler. Material monolit disintesis melalui metode sol-gel dengan menggunakan trimetoksipropilsilana (TPMS) dan (3-glisidoksipropil)trimetoksisilan (GPTMS) sebagai prekursor silika, yang dimodifikasi dengan asam amino sebagai monomer organik untuk memperkenalkan gugus fungsional aktif pada permukaan. Struktur dan morfologi monolit yang dihasilkan diamati menggunakan mikroskop elektron pemindai (SEM), sedangkan stabilitas mekaniknya dievaluasi melalui pengukuran penurunan tekanan (pressure drop) pada sistem aliran. Uji performa kromatografi dilakukan untuk menilai kemampuan kolom monolit dalam memisahkan dua analit model, yaitu urasil (senyawa polar) dan toluena (senyawa nonpolar). Hasil penelitian menunjukkan bahwa monolit yang disintesis memiliki morfologi berpori homogen dengan adhesi kuat pada dinding kapiler, serta menunjukkan stabilitas mekanik yang baik terhadap tekanan aliran. Kemampuan pemisahan yang ditunjukkan menandakan potensi material silika hibrid monolit ini untuk dikembangkan lebih lanjut sebagai fasa diam pada sistem capillary liquid chromatography (CLC).
Application of Thiol-Modified Silica Hybrid Monoliths for Hydrophilic Interaction Capillary Liquid Chromatography Furqani, Dr. Firda; Ilona Bella; Prima Fitria Hillman; Lee Wah Lim
Sains Natural: Journal of Biology and Chemistry Vol. 16 No. 1 (2026): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v16i1.911

Abstract

A silica hybrid monolithic column modified with Thiol-group were successfully prepared via “one-pot” approach; tetramethoxysilane and 3-mercaptopropyltrimethoxysilane were mixed together with the porogens in ice bath before being injected into the fused capillaries (0.032 mm I.D.). The composition of the porogens as well as the reaction time and temperature were optimized. The efficiency of the columns was evaluated by separating some polar biological compounds (i.e. thymine, uracil, adenine, adenosine, and cytidine) under hydrophilic interaction chromatography (HILIC) mode. Uracil, adenosine and cytidine were baseline-separated when the concentration of acetonitrile, which was used as the eluent, was more than 90%. It is also found that by increasing the acetonitrile concentration, the retention time of all analytes was increased, proving that typical HILIC separation mechanism was involved.
Co-Authors Furqani, Dr. Firda Furqani, Firda Ilona Bella Lee Wah Lim Syafrizayanti, Syafrizayanti Titisari, Geliz Luh