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All Journal Bioma : Jurnal Ilmiah Biologi Berita Biologi
Yogiara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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INDUCTION ENDOSPORE FORMATION OF Bacillus subtilis KM16 AS A PROBIOTIC CANDIDATE BY OPTIMIZATION OF THE GROWTH CONDITIONS Magdalena, Stella; Geraldine, Adelaide; Yogiara
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.418

Abstract

Probiotics are living microorganisms which have beneficial effects. One of the benefits is enhancing immunity. Therefore, probiotics are often supplemented into food, medical, and aquaculture products. B. subtilis KM16 as endospore-producing bacteria have been known to increase probiotic resistance to confront the unstable conditions of the digestive tract. Spore formation of Bacillus is supported by the optimal condition of growth medium and incubation periods. The sufficient availability of carbon, nitrogen, and mineral source in the growth medium, and environmental conditions can support the formation of optimal endospores. This research aimed to determine the suitable sugar composition of a medium and optimum incubation time suitable for endospores formation of B. subtilis KM16. Glucose and maltose were utilized as a carbon source in several concentration as a growth medium for B. subtilis KM 16. The incubation time was 48 and 72 hours. The viable number of vegetative cells was not significantly different for the type of sugar and incubation periods. Based on the glucose concentration, 0.2% glucose exhibited the uppermost number of cells, while 1% glucose concentration caused the decrease of vegetative cells. In this study, 0.2% glucose with 48 hours incubation period showed the highest percentage of sporulation frequencies, up to 48%, but it was not significantly different from the other glucose concentration. The medium that contains 0.2% glucose with 48 hours incubation period is the best medium for the utilization of B. subtilis KM16 cells and spores as probiotic candidates.
PEMANFAATAN SUPERNATAN BEBAS SEL BAKTERI ASAM LAKTAT PADA Escherichia coli DAN UMUR SIMPAN SAYURAN SEGAR Septiyani; Magdalena, Stella; Yogiara
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5392

Abstract

Selada dan kubis merupakan sayuran yang rentan kerusakan akibat bakteri E. coli. E. coli mampu menyebabkan diare pada manusia jika sayuran dikonsumsi secara mentah. Aplikasi supernatan bakteri asam laktat (BAL) pada kubis dan selada menjadi salah satu solusi untuk mengatasi kedua masalah tersebut. Tujuan dari penelitian ini yaitu mengevaluasi penggunaan supernatan bebas sel BAL dalam mereduksi E. coli untuk menghambat kerusakan pada sayuran segar. Supernatan TL7 dan H1 disemprotkan pada kubis dan selada;  disimpan pada suhu 25 ºC dan 10 ºC dan dilakukan pengamatan pada hari 1, 5, dan 7. Pada suhu 25 °C, selada berubah warna menjadi hijau tua, tekstur melunak, serta tumbuh kapang sedangkan kubis berubah warna menjadi kehitaman. Pada suhu 10 °C, selada dan kubis tidak berubah warna dan tekstur. Aplikasi supernatan bebas sel kedua isolat pada selada dan kubis yang disimpan pada suhu 25 °C  dan 10 °C mampu menghambat E. coli hingga penyimpanan hari ketujuh dengan kisaran 0,4 log CFU/g – 1,9 log CFU/g. Kombinasi penambahan supernatan isolat BAL dan penyimpanan pada suhu 10 °C memiliki kemampuan menghambat E. coli secara nyata hingga penyimpanan hari ketujuh dibandingkan perlakuan E. coli saja pada semua perlakuan.  
Co-Authors Geraldine, Adelaide Septiyani Stella Magdalena