Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jaringan Laboratorium Medis Jurnal Ilmiah Kesehatan (JIKA)
Nastiti, Hilari Rio Rosa
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Desain Primer untuk Deteksi Gen Diphtheria Toxin Repressor (dtxR) sebagai Biomarker Bakteri Corynebacterium diphtheriae Menggunakan In Silico PCR Nastiti, Hilari Rio Rosa; Kuncara, Rachmad Bayu
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10588

Abstract

Corynebacterium diphtheriae is the bacteria that causes diphtheria. The virulence factor of C. diphtheriae comes from the bacteria's ability to produce bacterial toxins. Toxin production is regulated by a set of genes called tox/dtx genes and is regulated by the dtxR gene. The aim of this study was to design primers used to evaluate the dtxR gene using bacterial DNA sequences. This research is experimental research with a literature study approach using the In silico Polymerase Chain Reaction (PCR), NCBI (National Center for Biotechnology Information), Primer3Plus, and Oligo Calculator applications. The sample obtained from genbank NCBI was C. diphtheriae dtxR gene M80337.1. In silico PCR examination was carried out using newly designed primers from Primer3Plus with 50 genomic DNA of Corynebacterium spp. taken from the In silico PCR database. The dtxR primer pair: '5-ACAGTTAGCCAAACCGTTGC-3' and 5'-TGCGTTCAACTTCGTCACTC-3' can produce a single DNA amplicon measuring 226 bp specifically for C. diphtheria types and no amplicon bands were generated from other Corynebacterium genomes. Based on the study results, this pair of specific primers can be used for in vitro PCR testing and can be used to develop rapid detection of diphtheria.
Evaluation of Mangosteen Peel Extract as a Substitute for Eosin 2% in Ascaris lumbricoides Egg Staining Nastiti, Hilari Rio Rosa; Widiyanto, SY Didik; Rosidah, Umi; Rokhani, Latifah Intan; Wahyuni, Sri; Handayani, Sih Rini
Jurnal Ilmiah Kesehatan (JIKA) Vol. 7 No. 3 (2025): Volume 7 Nomor 3 Desember 2025
Publisher : Sarana Ilmu Indonesia (Salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v7i3.1690

Abstract

Parasitic helminth infections remain a major public health problem in Indonesia, particularly among school-aged children. Tropical climatic conditions contribute to the high prevalence of Ascaris lumbricoides infection in endemic areas. Microscopic examination of helminth eggs commonly uses synthetic dyes such as eosin 2%; however, synthetic stains may pose potential health and environmental risks. This study aimed to evaluate the potential of mangosteen peel extract from Purbalingga (Garcinia mangostana L.) as a natural alternative stain for A. lumbricoides eggs. Mangosteen peel extract was tested at concentrations of 50%, 65%, and 80%, with eosin 2% used as a control. Staining quality was evaluated comparatively based on background contrast and clarity of egg morphology. The results showed that extract concentrations of 50% and 65% produced suboptimal visualization of A. lumbricoides eggs, whereas the 80% concentration provided better staining quality and clearer egg morphology, comparable to eosin 2%. Post hoc analysis demonstrated a significant difference between the eosin 2% control and the 50% extract concentration (p-value <0,001), while no significant differences were observed between eosin 2% and the 65% (p-value = 0,571) or 80% (p-value = 1,000) extract concentrations. These findings indicate that mangosteen peel extract at an 80% concentration has potential as a natural alternative stain to assist microscopic visualization of A. lumbricoides eggs. However, further studies are required to evaluate the reproducibility and chemical stability of the extract before its broader application in diagnostic parasitology laboratories.
Co-Authors Handayani, Sih Rini Kuncara, Rachmad Bayu Rokhani, Latifah Intan Rosidah, Umi Sri Wahyuni Widiyanto, SY Didik