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All Journal Jurnal Analis Kesehatan Jurnal Kesehatan Jaringan Laboratorium Medis Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan Academic Hospital Journal E-Amal: Jurnal Pengabdian Kepada Masyarakat
Kuncara, Rachmad Bayu
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Hubungan Kadar Vitamin D3 Dengan Derajat Kepositifan BTA Pada Penderita Tuberkulosis Paru Kuncara, Rachmad Bayu; Istiqomah, Anisa; Hartono, Rodhi; Priyatno, Djoko
Jurnal Analis Kesehatan Vol. 12 No. 2 (2023): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i2.3925

Abstract

Tuberkulosis paru adalah penyakit menular yang disebabkan bakteri Mycobacterium tuberculosis (MTB). Pemeriksaan mikroskopis Bakteri Tahan Asam (BTA) merupakan pemeriksaan yang penting untuk membantu diagnosis tuberculosis paru. Penderita tuberculosis paru dapat mengalami peningkatan jumlah BTA apabila mengalami defisiensi vitamin D. Vitamin D dapat menghambat multiplikasi MTB dalam makrofag dengan induksi peptida anti mikroba. Penelitian ini bertujuan untuk mengetahui hubungan kadar vitamin D3 dengan derajat kepositifan bakteri tahan asam pada penderita tuberkulosis paru. Jenis penelitian ini adalah observasional analitik dengan desain cross sectional. Sampel penelitian sebanyak 30 responden diambil dari Puskesmas Bangetayu, Puskesmas Tlogosari Wetan, dan Puskesmas Kedungmundu di Kota Semarang. Sampel tersebut diperiksa BTA secara mikroskopis dan pemeriksaan vitamin D3 dilakukan dengan metode Enzym Linked Immunosorbent Assay (ELISA). Hasil pemeriksaan BTA menunjukan sebanyak 12 (40,0%) penderita dengan hasil scanty, sebanyak 7 (23,3%) penderita positif 1, sebanyak 7 (23,3%) penderita positif 2 dan 4 (13,4%) penderita positif 3. Hasil pemeriksaan vitamin D menunjukan sebanyak 23 (76,7%) penderita dengan status defisiensi, 5 (76,7%) penderita dengan status insufisiensi dan 2 (6,7%) penderita dengan status sufisiensi. Data tersebut dianalisa secara statiskik menggunakan uji Spearman dengan hasil p-Value = 0,023 (p< 0,05) sehingga dapat disimpulkan terdapat hubungan kadar vitamin D3 dengan derajat kepositifan BTA pada penderita tuberculosis paru.
Edukasi Masyarakat sebagai Pengawas Menelan Obat (PMO) untuk Meningkatkan Kepatuhan Pengobatan Tuberkulosis Putri, Adita Puspitasari Swastya; Duri, Iin Desmiany; Kuncara, Rachmad Bayu; Auliya, Qurrotu A’yuni
Abdimas Galuh Vol 6, No 2 (2024): September 2024
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v6i2.14905

Abstract

Tuberkulosis merupakan penyakit menular penyebab kematian terbanyak, termasuk di Indonesia. Jawa Tengah menempati posisi tertinggi kedua dengan jumlah kasus terbanyak. Penyakit tuberkulosis dapat disembuhkan dengan melakukan pengobatan secara rutin dan teratur. Keberhasilan pengobatan tuberkulosis didukung oleh adanya peran Pengawas Menelan Obat (PMO) yang memantau dan mengingatkan penderita tuberkulosis untuk meminum obat secara rutin dan teratur hingga tuntas. Tujuan kegiatan ini adalah meningkatkan pengetahuan masyarakat melalui edukasi masyarakat sebagai PMO untuk meningkatkan kepatuhan pengobatan tuberkulosis di Kelurahan Penggaron Kidul Kecamatan Pedurungan Kota Semarang. Kegiatan yang dilakukan adalah edukasi kesehatan berupa penyuluhan dengan metode ceramah, tanya jawab, dan diskusi. Peserta diberikan leaflet tentang PMO sebagai media promosi kesehatan. Kegiatan di awali dengan pengerjaan soal pretest dan di akhiri dengan posttest untuk mengukur tingkat pemahaman peserta. Berdasarkan hasil kegiatan diketahui bahwa ada perbedaan antara tingkat pengetahuan peserta sebelum dan sesudah dilakukan edukasi. Terjadi peningkatan pemahaman warga masyarakat RT.06 RW.03 di Kelurahan Penggaron Kidul Kecamatan Pedurungan Kota Semarang yang cukup signifikan terhadap rata-rata nilai peserta. Peserta dapat menyebarkan seluruh informasi tentang PMO kepada keluarga dan masyarakat lain disekitarnya dengan menggunakan leaflet sebagai media promosi kesehatan.
PELATIHAN TEKNIK PENGAMBILAN, PENGEPAKAN DAN PENGIRIMAN SPESIMEN DAHAK (SPUTUM) UNTUK DIAGNOSIS TUBERKULOSIS BAGI KADER KESEHATAN DI KELURAHAN SENDANG MULYO Kuncara, Rachmad Bayu; Hadipranoto, Ichsan; Afriansya, Roni; Sulistyasmi, Wiwit
E-Amal: Jurnal Pengabdian Kepada Masyarakat Vol 4 No 2: Mei-Agustus 2024
Publisher : LP2M STP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47492/eamal.v4i2.3213

Abstract

Tuberkulosis merupakan penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis complex (MTBC). Strategi DOTS adalah pengawasan langsung pengobatan jangka pendek dengan keharusan setiap pengelola program tuberkulosis untuk memfokuskan perhatian (direct attention) dalam usaha menemukan penderita dengan pemeriksaan mikroskop. Tujuan dari kegiatan ini adalah peningkatan pengetahuan kader tentang teknik pengambilan, pengepakan dan pengiriman spesimen dahak (sputum) untuk diagnosis tuberkulosis bagi kader kesehatan di Kelurahan Sendang Mulyo. Metode: melakukan intervensi dengan meningkatkan pengetahuan kader kesehatan dengan memberikan penyuluhan teknik pengambilan, pengepakan dan pengiriman spesimen dahak. Hasil: terjadi peningkatan pengetahuan kader sebesar 70% dalam mengetahui tentang teknik pengambilan, pengepakan dan pengiriman spesimen dahak.
Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Zatalini, Karinta Syifa; Kuncara, Rachmad Bayu; Sugihantono, Anung
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Perbedaan Kadar Vitamin D3 pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Najiah, Dewina Zumrotin
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11379

Abstract

Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.
Perbedaan Kadar TNF-α pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Purlinda, Devi Etivia; Setyaji, Yoki; Sulistyasmi, Wiwit
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12789

Abstract

Pulmonary tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis undergo Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months, known as the intensive phase, patients receive a combination of OATs such as isoniazid, rifampicin, ethambutol, and pyrazinamide. TNF-α is a pro-inflammatory cytokine that plays a crucial role in granuloma formation to contain the spread of Mycobacterium tuberculosis. This study aims to determine the difference in TNF-α levels in pulmonary tuberculosis patients before and after one month of OAT therapy. The study used an observational analytic design with a prospective cohort approach without comparison. A total of 17 newly diagnosed pulmonary tuberculosis patients at Bangetayu, Kedungmundu, and Tlogosari Wetan Health Centers, Semarang City, were included. TNF-α levels in serum samples were measured using the ELISA method. The mean TNF-α level before OAT therapy was 110.09±100,48 pg/mL, while after one month of therapy, it decreased to 90,05±60,12 pg/mL. The statistical analysis using the Mann-Whitney test yielded a p-value of 0.228 (p 0.05), indicating that there was no statistically significant difference in TNF-α levels before and after one month of anti-tuberculosis drug (OAT) therapy. However, the mean TNF-α levels showed a decreasing trend, which, although not statistically significant, may suggest an initial immunological response to the treatment. The researcher recommends further studies with a larger sample size and longer observation period—such as until the end of the intensive phase or the completion of therapy—in order to obtain more representative and statistically significant results regarding TNF-α dynamics during tuberculosis treatment.
The Role of TNF-α (Tumor Necrosis Factor-alpha) in Pulmonary Tuberculosis Sulistyasmi, Wiwit; Fuzianingsih, Eka Noviya; Kuncara, Rachmad Bayu
Academic Hospital Journal Vol 7, No 2 (2025)
Publisher : Rumah Sakit Akademik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ahj.v7i2.106190

Abstract

Background: Tuberculosis (TB) remains a primary global health concern, with Indonesia accounting for approximately 10% of international cases. Tumor necrosis factor-alpha (TNF-α) plays a crucial role in the immune response against Mycobacterium tuberculosis, particularly in pulmonary tuberculosis (TB).Method: This article is a narrative literature review that examines the role of tumor necrosis factor-alpha (TNF-α) in pulmonary tuberculosis.Result:  This pro-inflammatory cytokine is essential for activating macrophages, promoting phagolysosome fusion, and supporting the formation and maintenance of granulomas—immune structures critical for containing infection. However, dysregulated TNF-α expression can be detrimental: excessive levels contribute to inflammation and lung tissue damage, while insufficient production—such as that induced by TNF-α inhibitor therapy—can lead to reactivation of latent TB. This narrative review examines the biosynthesis, immunological functions, and regulatory mechanisms of TNF-α in the context of pulmonary tuberculosis, highlighting its dual role in protective immunity and immunopathology.Conclusion: A deeper understanding of TNF-α modulation may inform the development of targeted, host-directed therapies that improve treatment outcomes while minimizing tissue damage.
Analysis of NLR and MLR Values in Patients with Pulmonary Tuberculosis and Pulmonary Tuberculosis with Diabetes Mellitus Kuncara, Rachmad Bayu; Sasangka, Parisade Galih; Sugihantono, Anung; Duri, Iin Desmiany; Qomariyah, Nurul; Kasiyati, Menik; Azahra, Sresta; Sulistyasmi, Wiwit
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.5149

Abstract

Tuberculosis (TB) infection can cause changes in hematological parameters such as neutrophils, monocytes, and lymphocytes. The neutrophil-to-lymphocyte ratio (NLR) and monocyte-to-lymphocyte ratio (MLR) are used as indicators of inflammation and immune response in infectious diseases, including TB. Diabetes mellitus (DM) as a comorbidity can exacerbate inflammation, potentially affecting NLR and MLR values in TB patients. This study aimed to determine the NLR and MLR values in patients with pulmonary TB and pulmonary TB with DM. This research used a quantitative observational design with a cross-sectional approach, involving 72 respondents from several primary health centers (Puskesmas) in Semarang City, divided into two groups of 36 individuals each. The sampling technique used was purposive sampling. The results showed that the mean NLR value in pulmonary TB patients was 2.20, and in pulmonary TB with DM patients was 2.89, with a significant difference (p=0.002). Meanwhile, the mean MLR value in pulmonary TB patients was 0.34 and in pulmonary TB with DM patients was 0.33, with no significant difference (p=0.752). These findings indicate that NLR is more sensitive than MLR in reflecting the inflammatory status and DM complications in TB patients. NLR can be used as an additional marker in the clinical monitoring of TB patients, especially those with DM comorbidity.
Desain Primer untuk Deteksi Gen Diphtheria Toxin Repressor (dtxR) sebagai Biomarker Bakteri Corynebacterium diphtheriae Menggunakan In Silico PCR Nastiti, Hilari Rio Rosa; Kuncara, Rachmad Bayu
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10588

Abstract

Corynebacterium diphtheriae is the bacteria that causes diphtheria. The virulence factor of C. diphtheriae comes from the bacteria's ability to produce bacterial toxins. Toxin production is regulated by a set of genes called tox/dtx genes and is regulated by the dtxR gene. The aim of this study was to design primers used to evaluate the dtxR gene using bacterial DNA sequences. This research is experimental research with a literature study approach using the In silico Polymerase Chain Reaction (PCR), NCBI (National Center for Biotechnology Information), Primer3Plus, and Oligo Calculator applications. The sample obtained from genbank NCBI was C. diphtheriae dtxR gene M80337.1. In silico PCR examination was carried out using newly designed primers from Primer3Plus with 50 genomic DNA of Corynebacterium spp. taken from the In silico PCR database. The dtxR primer pair: '5-ACAGTTAGCCAAACCGTTGC-3' and 5'-TGCGTTCAACTTCGTCACTC-3' can produce a single DNA amplicon measuring 226 bp specifically for C. diphtheria types and no amplicon bands were generated from other Corynebacterium genomes. Based on the study results, this pair of specific primers can be used for in vitro PCR testing and can be used to develop rapid detection of diphtheria.
Perbedaan Kadar Vitamin D3 pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Najiah, Dewina Zumrotin
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11379

Abstract

Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.
Co-Authors Anung Sugihantono Auliya, Qurrotu A’yuni Azahra, Sresta Djoko Priyatno, Djoko Duri, Iin Desmiany Fuzianingsih, Eka Noviya Hadipranoto, Ichsan Istiqomah, Anisa Kasiyati, Menik Najiah, Dewina Zumrotin Nastiti, Hilari Rio Rosa Nurul Qomariah Nurul Qomariyah Purlinda, Devi Etivia Putri, Adita Puspitasari Swastya Ririh Jatmi Wikandari Rodhi Hartono Roni Afriansya Sasangka, Parisade Galih Setyaji, Yoki Widiyanto, SY. Didik Wiwit Sulistyasmi Zatalini, Karinta Syifa