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All Journal Jurnal Reka Lingkungan Jurnal Penelitian Pendidikan IPA (JPPIPA)
Hutasoit, Leonard Raden
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology Physics

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

 1 
Polimorfisme Genetik dari Daerah 3'-Non Koding Region (3'-UTR) dari Gen HSP 70 pada Kerbau Moa (Bubalus bubalis) Rumanta, Maman; Hutasoit, Leonard Raden; Kunda, Rony Marsyal; Utami, Prasetyarti
Jurnal Penelitian Pendidikan IPA Vol 11 No 9 (2025): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i9.11685

Abstract

The Heat Shock Protein 70 (HSP70) gene, specifically the 3-Untranslated Region (3-UTR), is critical for regulating mRNA stability and translation under heat stress. This study aimed to identify genetic polymorphisms in the 3-UTR of the HSP70 gene in Moa buffalo (Bubalus bubalis) and evaluate its potential role in heat adaptation. A total of 65 hair follicle samples (55 Moa buffalo and 10 Banten buffalo) were collected, followed by amplification by PCR and sequencing of the PCR product of the 3'-UTR regions. Alignment results from the 3'-UTR region showed the presence of 2 major polymorphic SNPs, i.e g.1904C>A, and g.1910A>G. SNP (g.1904C>A) was found to be a cytosine (C) to adenine (A) substitution mutation type, and SNP g.1910A>G an adenine (A) to guanine (G) substitution. The discovery of 2 SNPs in the 3-UTR of the HSP70 gene in Moa buffalo is very important for the development of genetic markers, helping to understand the mechanism of heat adaptation, to the conservation and improvement of livestock performance, especially Moa buffalo living in tropical environments.
Self-evaluation in Distance Education of Biology Program FKIP-Universitas Terbuka Hutasoit, Leonard Raden; Ratnaningsih, Anna; Iryani, Krisna; Wahyuningsih, Tri
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.4043

Abstract

The purpose of this research is expected to help the S1 Biology Education Study Program FKIP-UT in improving academic and administrative services for users. In detail, the objectives of this study are: (1) The effectiveness of the services that have been provided by the Biology Education S1 Study Program FKIP-UT to service users; (2) Student services of the S1 Biology Education Study Program FKIP-UT; (3) Service user satisfaction of the S1 Biology Education Study Program FKIP-UT; (4) Characteristics of the learning process of the S1 Biology Education Study Program FKIP –UT; (5)  Learning experience of service users of  the S1 Biology Education Study Program FKIP-UT. The research used quantitative and qualitative methods. Quantitative methods are carried out by surveying all graduates/ students using questionnaires [letters, forms]. While the qualitative method was carried out for limited respondents who were randomly selected proportionally through [online] interviews. The results of this study include data analysis of the effectiveness of services that have been provided by the Biology Education S1 Study Program FKIP-UT to service users, as many as 62.4% are satisfied with the Subject Material Books used, 48.5% are online tutorials, 41.3% Webinars and 56.2% are final exams. As many as 62% of respondents already know the student services of the S1 Biology Education Study Program FKIP-UT regarding reasoning, interests and talents, 60% regarding career guidance and 45.8% regarding welfare and easy access.  Satisfaction with the learning experience in the S1 Biology Education Study Program, respondents were satisfied and very satisfied in discussions, tutorial assignments, practices and practicums. With respondents who are dissatisfied with academic and administrative services, it is necessary to improve services and socialization for those who have difficulty accessing services.
Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration Kunda, Rony Marsyal; Rumanta, Maman; Hutasoit, Leonard Raden
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7109

Abstract

Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.
Co-Authors Anna Ratnaningsih, Anna Annisa, Indri Gusti Nurdin Krisna Iryani, Krisna Maman Rumanta Rony Marsyal Kunda Tri Wahyuningsih Utami, Prasetyarti