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The Optimization of Chlorophyll Extraction from Ulva Sp. with Ultrasound-Assisted Liquid Biphasic Systems Method Susilowati; Humaidi, Fakhri Arkaan; Fachri, Boy; Palupi, Bekti; Rizkiana, Meta Fitri; Rahmawati, Istiqomah; Amini, Helda Wika
Indonesian Journal of Science and Education Vol. 8 No. 1 (2024): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijose.v8i1.1085

Abstract

Ulva sp. is a type of green algae that is easily found in the shallow seas of Indonesia and contains various bioactive compounds. Chlorophyll is a bioactive compound that functions as a natural dye, free radical scavenger, and antioxidant in the body. Chlorophyll extraction with conventional methods requires a relatively long time and large amount of solvent. In this research, chlorophyll extraction from Ulva sp. with the Ultrasound-Assisted Liquid Biphasic System (UALBS) method. Ulva sp. as much as 2.5 grams mixed in acetone solvent, added K2HPO4 solution, and carried out the sonification process in a dark room. The extraction process performs at a parameter range of 5-15 minutes, ratio 0.05-0.1 g/mL, and 60-100 mesh particle size. The extracted filtrate was added with petroleum ether and distilled water to form a biphasic condition. The extracted chlorophyll was analyzed with chromatography and spectrophotometry method. Antioxidant activity was analyzed using the DPPH method. The optimum result of Ulva sp. chlorophyll extraction at an extraction time of 10 minutes, the ratio of solids to solvent was 0.1, the particle size of 60 mesh produced a yield of 1.88% with chlorophyll a 20.13664%, chlorophyll b 21.58672% and total chlorophyll 41.71012%, and the percentage inhibition 45.32%.
Socialization of Vegetable Waste Processing into Eco-Friendly Fish Pellets at SMK Muhammadiyah 7 Gondanglegi Rahmawati, Istiqomah; Ansori; Arie Setiawan, Felix; Palupi, Bekti; Fachri, Boy; Rizkiana, Meta; Mumtazah, Zuhriah; Kharisma Yolanda Putri, Ditta; Dwi Lestari, Fitria; Naufal Nizaar Aziz, Mohamad; Pramasta, Ady
Jurnal Inovasi Sains dan Teknologi Untuk Masyarakat Vol. 4 No. 1 (2026): Mei
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v4i1.60007

Abstract

Vegetable waste is often discarded without further processing, which can lead to environmental pollution. In fact, this waste still contains 12.64%–23.5% crude protein and 20.76%–29.18% crude fiber, which can be repurposed into an environmentally friendly, value-added product: fish pellets. The lack of understanding regarding this waste poses a challenge, necessitating alternative solutions. This outreach method serves as an educational solution to enhance understanding of the potential of vegetable waste as a raw material for fish pellets. The outreach activity was conducted at SMK Muhammadiyah 7 Gondanglegi and was attended by students from various departments. The implementation stages included preparation, outreach, and evaluation. During this activity, participants were provided with materials on the benefits of vegetable waste and the steps for processing it into fish pellets. A question-and-answer session followed this to allow participants to explore the material in greater depth. The results of this activity have tangibly improved participants' understanding of processing vegetable waste into fish pellets. Participants played an active role and participated enthusiastically in this activity, creating a positive impact on both the participants and the school. Consequently, this activity can be further developed with the school's support to ensure the program's sustainability.