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All Journal Jurnal Mandala Pharmacon Indonesia Jurnal Perikanan
Ibrahim, Nurul Fahmi
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Isolasi dan Identifikasi Bakteri Endofit Penghasil Antimikroba Asal Daun Mangrove Rhizopora apiculata Asal Kota Palopo Annisa, Rahmawati Nur; Sakaria, Fajria Sari; Umar, Anugrah; Ibrahim, Nurul Fahmi; Rahmat, Muh. Alif
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 2 (2024): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i2.629

Abstract

Mangrove adalah ekosistem yang kaya akan senyawa bioaktif dan merupakan ekostem bagi komunitas mikroorganisme penghasil antimikroba. Salah satu tumbuhan mangrove ang menjadi tempat hidup bagi bakteri endofit yaitu Rhizopora apiculata. Penelitian ini bertujuan pada pencarian dan identifikasi bakteri endofit asal daun mangrove R. apiculata yang dapat dijadikan sebagai sumber antimikroba. Isolasi bakteri endofit dilakukan dengan menggunakan metode pengenceran hingga 10-5 lalu ditanam di media NA. Identifikasi bakteri endofit mengacu pada karakteristik makroskopis, mikroskopis, dan profil biokimia yang sesuai dengan panduan identifikasi Bergey’s Manual of Determinative Bacteriology. Aktivitas antimikroba diuji menggunakan metode difusi cakram. Tiga isolat bakteri endofit, yakni RaWT1, RaWT2, dan RaWT3, berhasil diisolasi dari daun mangrove R. apiculata asal kec. Wara Timur Kota Palopo dan telah berhasil diidentifikasi sebagai genera Bacillus spp., Pseudomonas spp., dan Staphylococcus spp.. Hasil uji menunjukkan bahwa ketiga isolat bakteri memiliki spektrum aktivitas antimikroba yang berbeda. Isolat RaWT1 memiliki aktivitas yang kuat hanya terhadap E. coli ATCC-25922 dengan besar zona hambat 11.12 mm, sementara isolat RaWT2 dan RaWT3 lebih aktif terhadap S. aureus dengan diameter zona hambat berturut-turut yaitu 10.08 mm dan 10.83 mm yang tergolong dalam spektrum moderat.
ANTIMICROBIAL POTENTIAL OF Acanthus ilicifolius MANGROVE LEAF EXTRACT AGAINST PATHOGENIC BACTERIA Escherichia coli Ibrahim, Nurul Fahmi; Annisa, Rahmawati Nur; Septiyawati, Funty
Jurnal Perikanan Unram Vol 16 No 1 (2026): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v16i1.1777

Abstract

This research was motivated by the need for alternative natural treatments for bacterial infections, particularly Escherichia coli, a pathogen that causes gastrointestinal and urinary tract infections. The mangrove plant Acanthus ilicifolius is known to contain bioactive compounds such as flavonoids, alkaloids, and polyphenols, which have antibacterial potential. Therefore, this study aimed to determine the antimicrobial potential of Acanthus ilicifolius leaf extract against E. coli and to determine the most effective extract concentration in inhibiting its growth. The study was conducted experimentally in a laboratory using the agar diffusion method. Leaf samples were collected from the coast of Wara Selatan District, Takalala Village, Palopo City, then dried, ground, and extracted using a maceration method with 96% ethanol. The resulting extract was tested against E. coli at four concentrations: 15%, 30%, and 60%. Paper disks containing concentrated Acanthus ilicifolius extract were then placed on agar media that had been inoculated with E. coli test bacteria, then incubated for 24 hours at 37°C. After that, observations and measurements of the inhibition zone were carried out with a caliper. The results showed that Acanthus ilicifolius leaf extract had antibacterial activity against E. coli, which was indicated by the formation of an inhibition zone around the paper disk. The average inhibition zone ranged from 10.5 mm to 12.5 mm. A concentration of 30% produced the largest inhibition zone of 12.5 mm, indicating the highest effectiveness, although there was no tendency that higher concentrations produced larger inhibition zones. This indicates that the antibacterial effectiveness of the extract is not linear with increasing concentration.
Co-Authors Annisa, Rahmawati Nur Anugrah Umar Rahmat, Muh. Alif Rahmawati Nur Annisa Sakaria, Fajria Sari Septiyawati, Funty