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Yulianto, Agus Tri
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Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Morphological Identification of Bacteria from Tuna Fish Isolates (Thunnus sp.) in Kondang Merak Yulianto, Agus Tri; Yamindago, Ade
Aquatic Life Sciences Volume 1 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/aqlis0101218

Abstract

Bacteria are microscopic organisms, and a small portion of them are pathogenic or harmful to living organisms. One example is bacteria that cause damage and decay in captured Tuna fish (Thunnus sp.) off the coast of Kondang Merak, Malang. Therefore, to determine the genus of bacteria found in Tuna fish (Thunnus sp.), bacterial morphology identification was conducted. Bacterial morphology identification was performed using methods involving the identification of colony and cell morphology, as well as bacterial respiration tests to enhance genus prediction accuracy. Bacterial morphology identification involved several testing stages, including Gram-staining, cell observation and measurement, motility testing, and bacterial respiration testing. The bacterial isolation samples from Tuna fish (Thunnus sp.) on TSA media consisted of 8 samples, namely 1a, 1b, 2a, 2b, 3, 4a, 4b, and 5, which were differentiated based on bacterial colony morphology. Based on the results of colony morphology identification, cell morphology, and respiratory testing, all 8 bacterial samples were manually identified with reference to identification books. The identification results showed that several samples had similar morphological characteristics. The bacterial morphology identification results for samples 2a and 2b were identified as belonging to the genus Aeromonas; samples 4a and 4b were classified into the genus Mesophilobacter; sample 1a was categorized into the genus Carnobacterium; and samples 1b, 3, and 5 belonged to the genus Vibrio.
Extraction of Active Compounds from Mangrove Snail Meat (Terebralia sulcata) as an Antibacterial Agent Against Escherichia coli Yulianto, Agus Tri
Aquatic Life Sciences Volume 1 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/aqlis0101229

Abstract

Bacterial Resistance is defined as the inability of antibiotics to inhibit bacterial growth systemically, mainly caused by synthetic chemical antibiotics. Therefore, research on pharmacological alternatives to synthetic antibiotics with natural antibacterial agents is needed, one of which is the crude extract of mangrove snail (T. sulcata) tested against Escherichia coli (E. coli) bacteria. This study conducted phytochemical screening and antibacterial potential testing of T. sulcata meat extract. T. sulcata was extracted with a polar solution of Methanol pa 1:4. Antibacterial testing against E. coli was performed at different extract concentration levels (5,000 ppm - 20,000 ppm). The results showed antibacterial activity with the formation of inhibition zones in the E. coli bacterial suspension medium. ANOVA analysis indicated that the mangrove T. sulcate extract at various concentrations influenced the growth of E. coli bacteria. Differences in treatment demonstrated an increase in extract concentration, which was directly proportional to the diameter of the inhibition zone. Observations of changes in the inhibition zone indicated that T. sulcata extract exhibited bacteriostatic properties against E. coli, characterized by a reduction in the diameter of the inhibition zone at each treatment concentration level. The formation of inhibition zones is believed to be due to the activity of active compounds such as alkaloids, flavonoids, saponins, phenols, and terpenoids.
Co-Authors Ade Yamindago