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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Septian, Wike Andre Septian
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Identification of Genetic Diversity of P21-Activated Kinases (PAK1) Gene in Senduro Goats and Boerawa Goats using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) Heri Damayanti; Susilorini, Tri Eko; Prasetyo, Guruh; Hidayah, Nur; Efendi, Ahmad Zarkasi; Ardiantoro, Ari; Septian, Wike Andre Septian; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.06

Abstract

This study aimed to detect genetic diversity associated with litter size traits of the P21-activated kinases (PAK1) gene in Senduro and Boerawa goats using the PCR-RFLP method. The restriction enzyme used is MSP1 (C*CGG). A pair of primers used in this study, namely Forward ((5'-GCTCAGTGTTGTATTAGCAG-3') and Reverse (5'- CAAGATATAAAGCACAGCCG-3') product length 713 bp (access code ENSCHIG00000003071). The samples used in this study consisted of 80 samples of Senduro goats and 20 samples of Boerawa goats from UPT PT-HMT Singosari, Malang Regency. The results showed that the PAK1 exon 9 gene in Senduro goats and Boerawa goats UPT PT-HMT Singosari was uniform (monomorphic), it was because all samples had GG genotype and G allele frequency of 100%. In conclusion, the PAK1 exon 9 / MSP1 gene is monomorphic and does not associate with litter size in Senduro goats and Boerawa goats, so it cannot be used as a genetic marker.
Co-Authors Ardiantoro, Ari Efendi, Ahmad Zarkasi Heri Damayanti Nur Hidayah Prasetyo, Guruh Suyadi Suyadi Tri Eko Susilorini