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All Journal Meditory : The Journal of Medical Laboratory
Putri, Rury Erina
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Biochemistry, Genetics & Molecular Biology Environmental Science

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IDENTIFICATION OF HUMAN HAIR USING DIRECT PCR TARGETING THE CYTOCHROME B GENE Tri Widodo, Wimbuh; Masjkur, Indah Nuraini; Huda, Qurrota A’yunil; Yudianto, Ahmad; Kristianto, Sonny; Putri, Rury Erina
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3784

Abstract

Background: Hair is one of the human parts that is easily scattered and easy to obtain for testing. Direct PCR which is PCR without going through the DNA extraction process has been widely used in various fields. The cytochrome b gene is one of the genes located in mitochondrial DNA. The gene is unique in that it has a small variation within one species organism but has a large variation between species. Aims: c. Methods: Hair was heated at 600C for 10 minutes then the sample was used for the PCR template. PCR was performed using the cytochrome b gene. The results: The analysis showed that human hair could still be detected on day 30 using direct PCR using the cytochrome b gene. Conclusions: This result will simplify and save time in analysis in various fields related to hair shafts.
Co-Authors Ahmad Yudianto Huda, Qurrota A’yunil Indah Nuraini Masjkur Kristianto, Sonny Wimbuh Tri Widodo