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All Journal Folia Medica Indonesiana Jurnal Biosains Pascasarjana Meditory : The Journal of Medical Laboratory Jurnal Kreativitas PKM Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Cendekia : Jurnal Pengabdian Masyarakat Medicra (Journal of Medical Laboratory Science/Technology) Jurnal Kesehatan Tambusai Jurnal Pengabdian Masyarakat (ABDIRA) Journal of Natural Sciences and Learning PENITI BANGSA (Pemanfaatan Ilmu Pengetahuan dan Teknologi bagi Masyarakat)
Wimbuh Tri Widodo
Magister Ilmu Forensik, Sekolah Pascasarjana, Universitas Airlangga Surabaya
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Identification of Human DNA in Mixture of Human and Chicken Blood Using PCR with Specific Primer of Cytochrome B Gene Wimbuh Tri Widodo; Ahmad Yudianto; Sri Puji Astuti W
Folia Medica Indonesiana Vol. 54 No. 3 (2018): September
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.888 KB) | DOI: 10.20473/fmi.v54i3.10009

Abstract

This study aimed to identify human DNA from mixing human and chicken blood samples by utilizing Polymerase Chain Reaction (PCR) and cytochrome b gene primer. The cytochrome b gene is a gene located in mitochondrial DNA and has high variation of sequence relation between one species and another. PCR analysis was performed using human cytochrome b gene primer in variation of DNA templates (0 ng, 0.01 ng, 0.1 ng, 1 ng, 10 ng and 100 ng), human blood percentages (100%, 50%, 40 %, 25%, 10%, 5%, 1%, 0%) and sample age before analysis (0 day, 3 days, 7 days, 10 days, and 15 days). The minimum DNA template obtained in this study was 0.01 ng and minimum percentage of human blood in the mixture was 1%. Blood spots on cloth isolated on days 0, 3, 7, 10 and 15 could still be analyzed and the resulting of DNA band (157 bp) had the same intensity/thickness. From the results of this study, it can be concluded that human blood in the mixture of human and chicken blood can be identified using PCR with specific primers of cytochrome b gene. PCR using specific primer of cytochrome b gene may help forensic practitioners to identify human sample in mixed biological samples.
Detection of Escherichia Coli Using PCR Analysis Without DNA Extraction Wimbuh Tri Widodo; Choirul Huda
Folia Medica Indonesiana Vol. 57 No. 2 (2021): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.536 KB) | DOI: 10.20473/fmi.v57i2.22097

Abstract

This study aimed to detect Escherichia coli directly without DNA extraction. The nucleus membrane and cell membranes of the Escherichia coli are composed of a phospholipid bilayer, damaged if heated at 950C. Pre-denaturation and denaturation of PCR were carried out at 950C. The two stages are thought to break down the Escherichia coli cells, so that the DNA that comes out of the cells can directly become a template in the PCR analysis. In this study, PCR analysis was carried out using Escherichia coli culture, Escherichia coli bacteria culture incubated at 950C, and Escherichia coli bacteria cultures incubated at 650C + on ice as templates. The results showed that PCR analysis using Escherichia coli culture directly and Escherichia coli culture incubated at 650C + on ice as templates produced very thin DNA bands with a size of 580 bp. while PCR analysis using Escherichia coli bacteria culture incubated at 950C as a template produced thick DNA bands with a size of 580 bp. This study's results are very useful for saving time and costs in the detection of Escherichia coli bacteria. The sample to be tested does not need DNA isolation as usual, but only needs to be incubated at 950C for 10 minutes.
RELATIONSHIP BETWEEN HbA1C AND eGFR IN DIABETES MELLITUS (DM) PATIENTS FOLLOWING PROLANIS AT ULTRA MEDICA TULUNGAGUNG CLINIC LABORATORY taufik salis syaifuddin; Mutia Hariani Nurjanah; Nurul Chamidah Kumalasari; Wimbuh Tri widodo
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.13-20

Abstract

Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged hyperglycemia and elevated HbA1c levels as a standard test for diabetes mellitus. HbA1c describes the level of sugar in the blood for the last 3 months. One of the complications of diabetes mellitus is kidney damage. The state of kidney function can be described from the GFR (Glomerular Filtration Rate) examination as a standard examination for abnormalities in kidney disease. The purpose of this study was to determine the relationship between HbA1c levels and eGFR values ​​in people with diabetes mellitus. The design of this study was a cross-sectional approach to 30 samples of DM patients who took Prolanis at the Ultra Medica Tulungagung Clinical Laboratory. The results showed that the average HbA1c level in all samples was 6.2% and the average eGFR value was 88.7 ml/min/1, 73m2. The Pearson correlation test obtained a ρ value = 0.000 so that it can be concluded that there is a relationship between HbA1c levels and eGFR values ​​in people with diabetes mellitus who take Prolanis at the Ultra Medica Clinical Laboratory Tulungagung.
Education on Making Floor Cleaning Soap from Used Cooking Oil (SUPERMIJEL) Rahma Diyan Martha; Fatimah Fatimah; Nurul Chamidah Kumalasari; Wimbuh Tri Widodo; Danar Danar
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 6 No. 1 (2023): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35568/abdimas.v6i1.2854

Abstract

Waste cooking oil is also called used oil used for frying. The use of used cooking oil can have an impact onour bodies, and indiscriminate disposal of the waste can pollute the environment and waters. So, there needsto bea smart solution to overcome this problem. This community service activity aims to socialize thedangers of used cooking oil and provide knowledge about other benefits of used cooking oil. The method used in this activity is the delivery of materials and direct practice of making products. The evaluation of thisactivity can be seen from the questionnaires distributed before and after theactivity started. The results of the evaluation activities were an increase in knowledge about the dangers of using cooking oil repeatedly for health. In addition, residents gained new knowledge and direct practice about other benefits of usedcooking oil, namely, floor cleaning soap from usedcooking oil. This community service activity can be followedby the participants well and with highenthusiasm
Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus: Efektivitas Ekstrak Etanol Daun Lidah Buaya (Aloe vera) terhadap Staphylococcus aureus Permatasari, Viki Ayu Intan; Nurjanah, Mutia Hariani; Widodo, Wimbuh Tri
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 3 No. 2 (2020): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v3i2.760

Abstract

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations
Analysis Of Bacterial Contamination By Total Plate Number (ALT) Method In Smoked Fish At One Of The Market Traders: Analisis Cemaran Bakteri Metode Angka Lempeng Total (ALT) pada Ikan Asap di Salah Satu Pedagang Pasar Safitri, Yunita Diyah; Widodo, Wimbuh Tri; Azizah, Yunita Dwi Nur
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i2.1723

Abstract

Bacterial contamination can come from the environment as a result of food production processes in the form of biological, chemical and foreign matter contaminants that can interfere with, harm and endanger human health. One of the problems in the processed food sector is the high level of bacterial contamination in the food served on the market. The condition of open food is also the main factor causing food to be contaminated with bacteria. The Total Plate Count (ALT) or total plate count (TPC) is an examination used to determine the number of microorganisms both bacteria and fungi using solid media with the final result being colonies. The aim of the study was to determine bacterial contamination and to determine the total plate number (ALT) of bacterial colonies in smoked fish sold at Ngemplak Market, Tulungagung. This research used a Simple Random Design (RAS) method with four treatments and 3 repetitions. The results showed that for menot fish it was 16.2 x 105 cfu/g, for tuna it was 10.5 x 105 cfu/g, for tuna it was 9.4 x 105 cfu/g, for bader fish it was 16.4 x 105 cfu/g. g, and in whitefish 20.5 x 105 cfu/g. The conclusion is that the bacterial contamination of smoked fish sold at the Ngemplak Market using the ALT method exceeds the normal limit value set by SNI 2725:2013.
Identification Of Mold Contaminan In Expired Wet Bread In Tulung Agung Tri Widodo, Wimbuh
Journal of Natural Sciences and Learning Vol. 3 No. 1 (2024): Journal of Natural Science and Learning
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jnsl.v3i1.131

Abstract

Bread is a carbohydrate source that is considered very practical for today's fast-paced and practical life. Mold is one of the microorganisms that cause disease in humans. Mold is the main microorganism that plays an important role in the process of making and spoiling bread. Fungi that are often found in bread spoilage are Rhizopus stolonifer, Penicillium s, and Aspergillus sp. This study used a descriptive research design. The purpose of this study was to determine the presence of mold on wet bread that is no longer suitable for consumption in Tulungagung Regency. The population in this study was bread sold in Tulungagung Regency. The samples of this study were 4 types of bread that are often sold in Tulungagung Regency. pandan brown bread samples contained Aspergillus sp and Rhizopus sp fungal species, banana bread samples contained Aspergillus sp and Rhizopus sp fungal species, steamed bread samples contained Aspergillus sp and Rhizopus sp fungal species, and putu ayu bread samples contained Aspergillus sp and Rhizopus sp fungal species. The conclusion of this research was that the fungal species of Rizhoppus sp and Aspergillus sp were found. This shows that bread that is not suitable for consumption can cause health problems for consumers
Peningkatan Literasi Narkotika dan Psikotropika melalui Edukasi dan Pembentukan Satgas Anti Narkoba di SMPN 1 Pacet Kabupaten Mojokerto Kristianto, Sonny; Yudianto , Ahmad; Putri , Rury Eryna; Widodo , Wimbuh Tri; Ripki, Nadia; Zulqaida, Salma
PENITI BANGSA (Pemanfaatan Ilmu Pengetahuan dan Teknologi bagi Masyarakat) Vol 2 No 2 (2024): PENITI BANGSA
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/PENITI-BANGSA.v2i22024.422

Abstract

Drug abuse among adolescents continues to rise, particularly within school-aged groups. SMPN 1 Pacet in Mojokerto Regency, with students aged 13-15 years old, is an environment vulnerable to this risk. This community service activity aims to increase students' literacy about the dangers of narcotics and psychotropic drugs. This program involved 50 Grade 9 students along with the Osis/MPK and presented experts from Social Service and Airlangga University. Methods included a pre-test, education, practice of urine drug testing using rapid diagnostic tests independently and post-test. The results showed an increase in the average score of students' understanding by 12.4%, as well as the formation of the Anti-Drug Task Force as an agent of change. This success supports the goals of SDG 4 (quality education) and SDG 16 (peace, justice, and strong institutions). This program demonstrates the effectiveness of participatory-based education in creating a drug-free school environment.
Edukasi Optimalisasi Bahan Alam sebagai Ramuan Herbal Kesehatan Reproduksi Remaja di MA Permata Kota Mojokerto Putri, Rury Eryna; Kristianto, Sonny; Yuliani, Gandul Atik; Abid, Istiana Firqah; Afdhila, Zakia; Uktutias, Sendy Ayu Mitra; Zulqaida, Salma; Widodo, Wimbuh Tri
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 8, No 4 (2025): Volume 8 No 4 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v8i4.18733

Abstract

ABSTRAK Bahan alam Indonesia dilaporkan memiliki beragam senyawa aktif seperti alkaloid, flavonoid, dan fenolik, yang menunjukkan aktifitas antibakteri dan antioksidan yang tinggi sehingga dimanfaatkan sebagai ramuan untuk menjaga kesehatan reproduksi. Namun, kurangnya perilaku perawatan diri sejak remaja dapat disebabkan oleh kurangnya kesadaran dan informasi yang memadai tentang cara menjaga kebersihan diri pada masa tersebut. Termasuk rendahnya kesadaran penggunaan bahan alam herbal. Edukasi tentang peningkatan pengetahuan tentang bahan alam sebagai bahan ramuan kesehatan reproduksi kepada siswa di dalam sataun pendidikan menjadi langkah strategis untuk membina siswa memahami problema di atas. Program ini menyasar siswa putri kelas X, Madrasah Aliyah (MA) Permata Kota Mojokerto. Metode pelaksanaannya meliputi tahap persiapan (survey awal), pelaksanaan program edukasi dan demo pembuatan jamu, lalu evaluasi. Berdasarkan hasil evaluasi berupa skor pre- dan post-test, didapatkan peningkatan signifikan performa siswa berupa pengetahuan bahan alam, ramuan yang dapat dibuat dari bahan alam, dan khasiat bahan alam untuk kesehatan reproduksi, yang ditandai dengan pergeseran distribusi skor ke rentang 90-100 atau skor maksimal. Melalui program edukasi di MA Permata Kota Mojokerto ini dapat menumbuhkan kesadaran dan pengetahuan yang baik bahan alam serta pembuatan dan penggunaan ramuan herbal yang tepat untuk menjaga kesehatan reproduksi Kata Kunci: Herbal, Learning Outcomes, Jamu, Student Awareness  ABSTRACT Indonesia's natural resources are reported to contain various active compounds such as alkaloids, flavonoids, and phenolics, which exhibit high antibacterial and antioxidant activities. Those natural resources are valuable as herbal remedies for maintaining reproductive health. However, a lack of self-care practices during adolescence can stem from limited awareness and insufficient information on personal hygiene, including a lack of understanding about the use of herbal natural resources. This program aims to educate students to increase their knowledge about natural resources as ingredients for reproductive health remedies, providing a strategic step to help adolescence address these issues. The program targeted female 10th-grade students of Madrasah Aliyah (MA) Permata, Mojokerto. The implementation included preparatory stages (preliminary surveys), program execution through educational sessions, herbal remedy-making demonstrations, and evaluations. Based on the evaluation, significant improvements were observed in students’ performance, reflected by increased knowledge about natural ingredients, herbal remedies that can be created from them, and their benefits for reproductive health. This was evidenced by a shift in score distribution to the 90-100 range, or the maximum score. This educational program at MA Permata Mojokerto successfully fostered awareness and understanding of natural ingredients, as well as the proper preparation and use of herbal remedies to support reproductive health. Keywords: Herbal Remedies, Learning Outcomes, Jamu, Student Awareness
KOMPONEN, TAHAPAN DAN VARIASI POLIMERASE CHAIN REACTION : ARTIKEL REVIEW Widodo, Wimbuh Tri
Jurnal Kesehatan Tambusai Vol. 6 No. 1 (2025): MARET 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v6i1.43342

Abstract

Polymerase Chain Reaction (PCR) merupakan teknik perbanyakan DNA secara in vitro yang memanfaatkan enzim DNA polymerase termostabil. PCR memiliki peran penting dalam berbagai bidang, terutama biomedis dan forensik, karena kemampuannya dalam mendeteksi serta mengidentifikasi DNA target dengan sensitivitas dan spesifisitas tinggi. Penelitian ini bertujuan untuk menjelaskan prinsip kerja PCR, komponen utama dalam reaksi PCR, tahapan PCR, serta variasi teknik PCR yang berkembang, seperti Real-Time PCR, Reverse Transcription-PCR (RT-PCR), dan direct PCR. Metode yang digunakan adalah studi literatur terhadap berbagai sumber ilmiah relevan. Hasil penelitian menunjukkan bahwa PCR terdiri dari tiga tahap utama, yaitu denaturasi, annealing, dan elongasi, yang dilakukan secara berulang-ulang hingga menghasilkan jumlah salinan DNA yang cukup untuk dianalisis. Berbagai variasi PCR telah dikembangkan untuk meningkatkan sensitivitas dan efisiensi deteksi. Real-Time PCR memungkinkan kuantifikasi DNA secara akurat, RT-PCR memungkinkan analisis RNA melalui konversi menjadi DNA, sementara direct PCR menyederhanakan proses dengan menghilangkan tahap ekstraksi DNA. Aplikasi PCR sangat luas, termasuk dalam diagnostik penyakit infeksi, forensik, penelitian genetik, deteksi mutasi genetik, identifikasi mikroorganisme, dan pengujian makanan. Keunggulan PCR dalam kecepatan, ketepatan, serta kemampuannya mendeteksi DNA dalam jumlah sangat kecil menjadikannya alat yang sangat penting dalam ilmu biologi molekuler. Kesimpulannya, PCR merupakan teknik fundamental dalam biologi molekuler yang terus berkembang, memberikan kontribusi besar dalam penelitian dan aplikasi klinis.
Co-Authors Abid, Istiana Firqah Afdhila, Zakia Ahmad Yudianto Aprilia, Chatrina Apriliani, Herlina Azizah, Yunita Dwi Nur Choirul Huda Danar Danar Danar, Danar Dyah Setyaningrum, Dyah Fadila, Natasya Nurul Fatimah Fatimah Huda, Qurrota A’yunil Indah Nuraini Masjkur Kristianto, Sonny Kumalasari, Nurul Chamidah Mutia Hariani Nurjanah Nurin, Wachidatun Nurjanah, Mutia Hariani Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Permatasari, Viki Ayu Intan Putri, Amalia Eka Putri, Rury Erina Rahma Diyan Martha Rahmadani, Anindita Mutiara Rayditya Milanio, Roderikus Ripki, Nadia Rury Eryna Putri Safitri, Yunita Diyah Setiawardani, Alvina Sri Puji Astuti W taufik salis syaifuddin Uktutias, Sendy Ayu Mitra ul Haq, Ihtisham ur Rahman, Anees Ur Rahman, Ateeq Yudianto , Ahmad Yuliani, Gandul Atik Zulqaida, Salma