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Test of the Effectiveness of Moringa Oleifera Leaf Extract Cream on the Healing Process of Cut Wounds on the Skin Surface of Male Wistar Rats (Rattus Norvegicus) Triastuti, Yulia; Nadya Nazimuddin Putri; Muhammad Nawal Hasya
Jurnal EduHealth Vol. 14 No. 04 (2023): Jurnal eduHealt, 2023, December
Publisher : Sean Institute

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Abstract

Everyone there must have been injured in some way. Moringa Oleifera leaves have significant antioxidant activity and are just one of many plant-based treatments used to treat pain and speed wound healing. This study aims to ascertain if a cream made from the extract of moringa leaves (Moringa oleifera) accelerates the recovery of damages caused by cuts. This study strategy employs an actual or laboratory experimental design based on a pre-post test with a control group. Twenty adult male Wistar rats (Rattus norvegicus) were used. Mice were acclimatized, phytochemicals were screened, and treatment and monitoring were performed using dermapen wounds during the investigation. The Kolmogorov-Smirnov test, One-Way Analysis of Variance (ANOVA), and LSD post hoc Test were used to examine the research data in SPSS. Results from a 14-day study on the effects of a cream containing Moringa leaf extract on the wound healing of male Wistar rats (Rattus norvegicus) led researchers to this conclusion. In a recent phytochemical study, flavonoids, saponins, tannins, and alkaloids, known to have therapeutic benefits due to their high antioxidant content, were found in Moringa leaf extract. The secondary metabolite chemicals found in Moringa leaf extract significantly affected wound healing, with the best effects shown at an extract level of 10%.
Anti-Bacterial Activity Test of Carrot Tuber Ethanol Extract Hydrogel Preparation (Daucus Carota L.) in Inhibiting the Growth of Bacteria and Their Ability in the Healing Process of Grade II Burns on Wistar Rat (Rattus Norvegicus) Welly Ria Utami Lubis; Ali Napiah Nasution; Hanjaya; Alvin Abrar Harahap; Muhammad Nawal Hasya; Jeri Yuliansyah; Ermi Girsang
Jurnal Multidisiplin Madani Vol. 3 No. 6 (2023): June, 2023
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/mudima.v3i6.3529

Abstract

Carrots are root vegetables that are very common in Indonesia. Carrot plant (Daucus carita L.)  is a root vegetable plant that has a high content of beta carotene, rich in dietary fiber, natural antioxidants and high vitamin A which is 12,000 IU. The reddish-yellow color of carrots is due to the high content of carotene pigment. The sample used in this study was fresh carrot tubers. The tubers are separated from other impurities and then washed thoroughly and then drained and weighed. Next, the tubers are dried at a temperature of 30-40oC until the tubers are dry (marked when broken brittle). Simplisia that has dried (brittle) is powdered with a blender and stored in a tightly closed container and stored at room temperature. The preparation of the extract is carried out by maceration using 70% ethanol solvent. According to the Indonesian Herbal Pharmacopoeia (2008), as much as 1 part of simplisia dry powder is put into the maserator, added 10 parts of solvent. Soak for the first 6 hours while stirring occasionally, then let stand for 18 hours. Separate the maserat by filtering. Repeat the extraction process at least twice with the same type and amount of solvent. Furthermore, all the mafiber is collected, then evaporated with a  rotary evaporator at a temperature of ±50oC until a thick extract is obtained. The results showed that ethanol extract of carrot tubers with a simplisia weight of 500 grams produced a yield of 3.78% with an extract weight of 68.9 grams. The results below show that carrot tuber ethanol extract shows phytochemical screening results that contain flavonoids, alkaloids, glycosides, tannins and steroids. The results of the measurement of the diameter of the inhibitory zone above showed that ethanol extract of carrot tubers (Daucus carota L.) can inhibit the growth of  Pseudomonas aeruginosa and Staphylococcus aureus  bacteria at concentrations of 10-400 mg / mL. The measurement results obtained the diameter of the inhibitory zone with a strong inhibitory zone response category at all concentration variations. It was found that high concentrations had activity that did not differ significantly from low concentrations. Carrot tuber ethanol extract has a significant difference (p < 0.05) when compared to the positive and negative groups while the results of the measurement of bacterial killing power above show that carrot tuber ethanol extract (Daucus carota L.) can inhibit the growth of Pseudomonas aeruginosa and Staphylococcus aureus bacteria  at concentrations of 10 – 100 mg / mLl