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OPTIMALISASI FERMENTASI ETANOL “TERATAI SALJU” DENGAN PALM SUGAR DAN VARIASI SUKROSA Sarwoko, Jonathan Puji; Sidharta, Boy Rahardjo; Mursyanti, Exsyupransia
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.12092

Abstract

Abstract "Teratai Salju" is a symbiotic community of bacteria and yeast. One of the fermentation results of "snow lotus" was alcohol that is useful as a solvent such as cosmetics, disinfectants, and fuels. This research aims to determine the genus of microorganisms and the maximum ethanol content produced by "teratai salju" using palm sugar and sucrose variations 15, 45 and 85 grams in 250 ml water with 5 repetitions. Stages of research including growth and maintenance of "snow lotus", observation of colonies morphology, yeast staining, negative staining, Gram staining, dry weight measurement, total acid number, pH measurement, and ethanol content measurement. Data analysis was performed by Analysis of variance (ANOVA) followed by a duncan multiple range test with a 95% confidence level with SPSS 15.0. This research was conducted with a Completely Randomized Design (CRD). The results obtained show that ethanol content of "teratai salju" optimized at the addition of 85 gram sucrose at 0,22-0,86%. Key Words : Ethanol, Fermentation, Sucrose Variation, “Teratai salju”.
Pola Distribusi Mikroba dan Pengaruhnya Terhadap Pembenihan Larva Udang Vanamei Di Hatchery Pantai Ketang Lampung Sarwoko, Jonathan Puji; Sumardi; Tugiyono; Nugroho Susanto, Gregorius; Irawan, Bambang
Jurnal Ekologi, Masyarakat dan Sains Vol 5 No 1 (2024): Jan-Jun 2024
Publisher : ECOTAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55448/f9ymg556

Abstract

Abstract: The microbial distribution pattern or dispersion of microbes in a water environment was the primary focus of this research. The study aimed to analyze the total density of Vibrio sp., coliform, and plankton in seawater, as well as the relationship between microbes and the hatching of vanamei shrimp larvae. The research was conducted from April to August 2022 at Pantai Ketang, Kalianda, Lampung, in the Microbiology and Zoology laboratory of the Faculty of Mathematics and Natural Sciences, Lampung University. The research employed an exploratory survey method, including the calculation of total Vibrio, coliform, plankton, and survival rates. Data analysis was carried out using analysis of variance, followed by the Duncan test (? = 0.05) for identifying significant differences among groups. Additionally, the microbial relationship was analyzed using Pearson correlation (? = 0.05). The results revealed that the highest Vibrio density occurred in May (3 CFU/ml) and in larval shrimp rearing water (M) (5 CFU/ml). Coliform density was less than < 3 MPN/100ml, and the highest plankton density was observed in August (2×102 ind/L) and in high tide seawater (P) (1×104 ind/L). However, there was a very low correlation between microbial density and the survival rate of vanamei shrimp larvae.