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Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Mursyanti, Exsyupransia; Lestari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, mediumâ€™s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncanâ€™s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 Âµg/ml) was on 18 hrs of incubation period.

Eksplorasi Genom, terkuaknya misteri manusia (Kajian Buku) Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Eksplorasi genom manusia yang terwadahi dalam Human Genom Project (HGP) telah berhasil mengidentifikasi keseluruhan genom (DNA) manusia dalam waktu 13 tahun (1990-2003), dua tahun lebih cepat dari yang ditargetkan (15 tahun). Karya yang spektakuler ini memberikan banyak informasi tambahan mengenai genom manusia. Genom manusia yang dahulu diperkirakan berukuran tiga milyar basa, ternyata tersusun atas 2,3 milyar nukleotida yang terdiri dari ±30.000 gen, 50% gen tersebut sudah diketahui fungsinya. Kromosom no.1 mengandung gen paling banyak (2.968 gen) sedangkan kromosom Y mengandung gen paling sedikit (231 gen). Selain itu, diinformasikan pula bahwa pembeda manusia yang satu dengan yang lain terletak pada tiga juta lokasi single nucleotide polymorphisms (SNPs).

Pola Pertumbuhan dan Produksi ï¡-Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Wahyuningsih, Sisilia Sri; Mursyanti, Exsyupransia; Atmodjo, P. Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

The aims of this studyÂ were to identify the growth curve ofÂ B. amyloliquefaciens onÂ corn-starch and non corn starch addition media,Â number of cellsÂ and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on bothÂ medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initialÂ pH of the media ( 5,Â 5.5,Â 6,Â 6.5,Â 7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production ofÂ a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cellsÂ (about 2.8542 x 108 cells/ml)Â and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hoursÂ incubation.

Mutu Bakteriologis Pindang Tongkol (Euthynnus affinis) yang Berasal dari Beberapa Pasar di Yogyakarta Purwijantiningsih, Ekawati; Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.2821

Food poisoning cases need to be examinated intensively. There have been five poisoning cases reported between 2003-2004 in Sleman area. Three of them were caused by contaminated tuna fish. The objective of this research was to determine bacteriological quality of salted tuna fish from several markets in Yogyakarta. Salted tuna fish sample were collected from five markets, namely Beringharjo, Condongcatur, Demangan, Kranggan and Sentul. In each market, four vendors were selected. Bacteriological test were done including total microbe cells utilizing PCA medium, Coliform test utilizing VRBA medium. Identification and isolation of Vibrio parahaemolyticus use pre-enrichment selective medium of Alkaline Petone Water and selective Chromogenic medium agar for Vibrio. Salmonella test utilizing Chromogenic agar continued by utilizing TSI and LIA medium. The result showed that bacteriological quality of salted tuna fish from markets in Yogyakarta did not fulfill the requirements based on SNI 01-2717-1992. It was shown that total microbial count were high, i.e. 2.4 x 105 â€“ 5.27 x 107 CFU/ g. Coliform count ranged from < 2.5 x 101 - > 6.5 x 106 CFU/g. From 20 samples that were brought to analyzed, 60% was positively presumed contaminated by V. parahaemolyticus and 50% was positively presumed contaminated by Salmonella.Â

Potensi Antibakteri Ekstrak Tanaman Suku Rubiaceae dan Aplikasinya dalam Sediaan Hand Sanitizer Rasid, Sriwahyuni Iskandar; Mursyanti, Exsyupransia; Sidharta, Boy Rahardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.4165

Tanaman suku Rubiaceae merupakan tanaman yang banyak digunakan sebagai obat tradisional. Kandungan senyawa aktif yang ada dalam tanaman suku Rubiaceae meliputi alkaloid, flavonoid, saponin, tanin, fenol, dan minyak atsiri (antrakuinon) yang berpotensi sebagai senyawa antibakteri. Kandungan alkohol dalam hand sanitizer yang beredar di pasaran memberi rasa iritasi dan terbakar pada kulit jika digunakan secara terus menerus sehingga inovasi hand sanitizer dari tanaman suku Rubiaceae dapat dijadikan alternatif untuk membersihkan tangan. Hasil literature review ini menunjukkan bahwa ekstrak dan sediaan hand sanitizer tanaman suku Rubiaceae yang diuji aktivitas antimikrobianya dengan metode difusi agar dan broth dilution mempunyai daya antibakteri terhadap S. aureus dan E.coli. Sediaan hand sanitizer tanaman suku Rubiaceae juga memiliki kualitas karakteristik fisik seperti bentuk, warna, dan bau sertaÂ stabilitas seperti pH, viskositas, homogenitas, daya lekat dan daya sebar yang baik. Kategori kualitas baik ini didasarkan pada kesesuaian SNI terkait kestabilan gel antiseptik.

Aktivitas Antibakteri Sediaan Sabun Wajah Cair Ekstrak Herba Pegagan (Centella asiatica (L.) Urban) terhadap Pertumbuhan Staphylococcus aureus dan Propionibacterium acnes Soebagio, Trisiana Tri; Hartini, Yustina Sri; Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 2 (2020): June 2020
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v5i2.2698

Salah satu penyakit kulit yang banyak dialami oleh remaja yaitu jerawat. Salah satu bakteri penyebab jerawat adalah Propionibacterium acnes. Oleh karena itu, jerawat dapat diobati dengan menggunakan antibiotik. Namun, penggunaan antibiotik yang berlebihan dapat menyebabkan munculnya efek samping seperti iritasi karena tidak cocok dengan kulit. Salah satu bahan alami tanaman obat yang dapat digunakan sebagai alternatif menggantikan antibiotik, adalah herba pegagan (Centella asiatica (L.) Urban). Bahan alami ini biasanya diaplikasikan dalam bentuk sediaan topikal. Tujuan penelitian adalah mengetahui kandungan flavonoid, tanin dan triterpenoid yang terdapat dalam ekstrak herba pegagan serta mengetahui karakteristik sabun wajah cair dengan penambahan ekstrak herba pegagan, dan mengetahui kemampuan sediaan sabun wajah cair ekstrak herba pegagan dalam menghambat pertumbuhan bakteri Staphylococcus aureus dan P. acnes. Perlakuan konsentrasi yang digunakan dalam pengujian aktivitas antibakteri ekstrak maupun sediaan sabun wajah cair ekstrak herba pegagan yaitu 20%, 30%, dan 40%, 50% dan 60%. Tahapan penelitian yang dilakukan yaitu identifikasi tanaman, pembuatan serbuk herba pegagan, ekstraksi herba pegagan, uji kualitatif dan kuantitatif ekstrak herba pegagan (flavonoid, tanin dan triterpenoid), pembuatan dan sterilisasi medium, uji kemurnian bakteri, uji aktivitas antibakteri ekstrak dan sediaan sabun wajah cair ekstrak herba pegagan, pembuatan sediaan sabun wajah cair dan uji evaluasi sediaan. Parameter evaluasi sediaan sabun wajah cair yaitu pH, tinggi busa, viskositas, dan homogenitas. Hasil yang didapatkan dari analisis fitokimia adalah ekstrak herba pegagan mengandung flavonoid dan tanin namun tidak mengandung triterpenoid. Kualitas dari sediaan sabun wajah cair ekstrak herba pegagan yang disimpan selama 28 hari dengan parameter pH, tinggi busa, viskositas dan homogenitas memenuhi SNI. Sabun wajah cair ekstrak herba pegagan memiliki daya antibakteri terhadap bakteri Staphylococcus aureus dan Propionibacterium acnes.

Kualitas Minyak Kelapa Hasil Fermentasi Saccharomyces cereviceae Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2880

One aspects of utilization of coconut fruit is its palm oil. Traditionally coconut oil was made by â€œklentikâ€ method, which was time and energy consuming. The important thing in the production of coconut oil from its xanthan is the separation of protein from oil. One of separation methods is by creating low pH (acid) condition that is near to protein isoelectric point. This research objective was to determine the potency of Saccharomyces cereviceae, that has an optimum growth pH within the range of protein isoelectric point, to produce coconut oil. Three isolates of Saccharomyces were used, i.e. isolate A that was coming from alcohol industry (S. cereviceae strain Kyowa), isolate B that was natural type (isolated from fruits), and isolate C that was S. cereviceae in the form of powder (commercial isolate, Saf-instant). Coconut xanthan used in the research was prepared from old coconut fruits. Research stages including pure culture test of S. cereviceae, starter preparation, xanthan production, fermentation process, oil separation and heating. Quantitative and qualitative analyses were done for oil rendement, water content, free-fatty acid concentration, saponification number, iod number, and organoleptic test (color and aroma). The results showed that the quality of coconut oil produced by fermentation process was better than traditional way in term of rendement value, water content, saponification number, and iod number. This was supported also by organoleptic test that aroma and color of coconut oil from fermentation were more preferred by the panelists. Furthermore, the quality of oil produced by S. cereviceae fulfilled the standart stated by the Indonesian Department of Commerce (SII-1976) except for saponification number.

Pelatihan Guru Biologi dan Siswa SD-SMA/SMK di Pontianak untuk Meningkatkan Pengalaman Belajar di Bidang Bioteknologi Mursyanti, Exsyupransia; Stefani Santi Widhiastuti; Dewi Retnaningati
Jurnal Atma Inovasia Vol. 2 No. 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Abstract — Pontianak is the center of education in the province of West Kalimantan. In the implementation of education and learning, the interaction between competent teachers and active students can improve the quality of education. Therefore, a service activity was made that aims to ensure quality educational services in accordance with the times. This service activity is entitled Training and Assistance for Biology Teachers and Elementary-SMA/SMK Students at Karya Yosep Elementary School, St. Peter's Middle/High School, St. Mary's Vocational School with the theme Biotechnology and its Applications for the Welfare of Human Life. The method used is contextual learning which is packaged with a webinar model which includes 2 sessions, namely the delivery of material and discussion sessions. This activity is divided into 3 stages, namely the preparation, implementation, and evaluation stages. Overall, the activity went smoothly. This activity can increase the knowledge of Biology teachers and students in partner schools about the field of biotechnology, and participants can practice and apply the material received in everyday life. More than 85% of the participants from the group of teachers, elementary-junior high school students, and high school/vocational school students thought that the material provided was in accordance with the needs.Keywords — training, teachers, students, biotechnology.

Pelatihan Pengolahan Produk dari Bunga Mawar pada Masyarakat di Kawasan Sapuangin, Merapi, Klaten Widhiastuti, Stefani Santi; Mursyanti, Exsyupransia; Sidharta, Bernardus Boy Rahardjo; Atmodjo, Patricius Kianto; Junedi, Sendy; Arsiningtyas, Ines Septi; To'bungan, Nelsiani
Jurnal Atma Inovasia Vol. 3 No. 6 (2023)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i6.8082

Roses are one of the commodities in the Sapuangin area, Pemalang, Klaten. The fluctuating selling price and short shelf life make fresh roses only a secondary product. Therefore, a training is needed to process rose flowers into products with high economic value and longer shelf life. This community service aims to enhance the community's knowledge in processing various products from rose flowers. The activities include survey, socialization, training implementation, and evaluation. The training consists of 7 topics from June to July 2023, where participants receive oral explanations and practical demonstrations of the materials provided. The evaluation includes assessing the participants' knowledge through questionnaires given at the beginning and end of the community service, as well as satisfaction evaluation. The entire community service runs smoothly, with participants able to practice making raw materials, solid soap, bath salts, aromatherapy candles, rosewater, and hand sanitizer. Participants also learn about the different types of roses. The average score of the questionnaires from 15 respondents before the training was 21.33 ± 2.61, increasing by 53.49% to 32.74 ± 3.90 from 19 respondents. 68.42% of participants expressed very satisfied, and 31.58% expressed satisfied with participating in the community service activities. In conclusion, the community service has improved the knowledge and skills of the Sapuangin Village community in creating products from rose flowers that have higher economic value and longer shelf life. Keywords — roses, soap, candles, hand sanitizer, rosewater Abstrak— Mawar merupakan salah satu komoditi di kawasan Sapuangin, Pemalang, Klaten. Harga jual yang tidak menentu dan masa simpan pendek menyebabkan mawar segar hanya menjadi produk sampingan. Oleh karena itu diperlukan kegiatan pelatihan pengolahan bunga mawar menjadi produk dengan nilai ekonomis tinggi dan masa simpan panjang. Pengabdian pada masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat dalam mengolah bunga mawar menjadi berbagai macam produk. Kegiatan meliputi tahap survey, sosialisasi, pelaksanaan pelatihan, dan evaluasi. Pelatihan terdiri dari 7 materi mulai Juni-Juli 2023, dimana peserta diberi penjelasan secara lisan dan mempraktekkan secara langsung materi yang diberikan. Evaluasi meliputi evaluasi tingkat pengetahuan melalui kuesioner yang diberikan di awal dan di akhir kegiatan pengabdian, serta evaluasi kepuasan. Seluruh kegiatan pengabdian berjalan lancar, peserta dapat mempraktekkan pembuatan simplisia, sabun padat, garam mandi, lilin aromaterapi, air mawar dan handsanitizer. Peserta juga mengetahui jenis-jenis bunga mawar. Nilai rata-rata kuesioner dari 15 responden sebelum pelatihan adalah 21,33 ± 2,61, meningkat sebesar 53,49% menjadi 32,74 ± 3,90 dari 19 responden. 68,42% peserta menyatakan sangat puas dan 31,58% menyatakan puas mengikuti kegiatan pengabdian. Dapat disimpulkan bahwa kegiatan pengabdian dapat meningkatkan pengetahuan dan kemampuan masyarakat Desa Sapuangin dalam membuat produk dari bunga mawar yang memiliki nilai ekonomis lebih tinggi dengan masa simpan panjang.

Pelatihan propagasi anggrek skala rumah tangga kepada kelompok wisata di kawasan Sapuangin, Merapi, Klaten Mursyanti, Exsyupransia; Nelsiani To’bungan; Pantalea Edeilweiss Vitara; Bernardus Boy Rahardjo Sidharta; Patricius Kianto Atmodjo; Sendy Junedi; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 4 No. 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Sapuangin area is one of supporting areas for Mount Merapi National Park (TNGM). The endemic Merapi orchid is widely cultivated by people in the Sapuangin, using conventional cultivation. Sapuangin’s youth, who are members of a tourism group (POKWIS) have never received training regarding orchid propagation using in vitro culture principles. This is important to support orchid conservation activities carried out by POKWIS in the Sapuangin greenhouse. Based on requests from the community, lecturers and educational laboratory staff concentrating on Technobio-Industrial Studies, Faculty of Biotechnology, Universitas Atma JayaYogyakarta, provided a series of training on in vitro orchid culture, for household scale. This activity is expected to provide knowledge and skills regarding orchid cultivation techniques for the community. The activity was held on 12-20 December 2023. The training activity was divided into several topics, namely introduction to orchid types and their uses, principles of in vitro orchid culture, introduction to tools, methods for sterilizing and sowing orchid seeds, pollination/pollination, overplanting, and acclimatization. The training carried out can provide additional knowledge for the community regarding in vitro orchid cultivation techniques. The implementation of the training was considered very useful and by community needs, the training resource was competent, the implementation time was by the plan, and was supported by complete facilities and infrastructure. The community hopes for ongoing guidance and assistance regarding orchid propagation in the future.

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