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All Journal ODONTO Dental Journal Odonto dental journal
Pratiwi, Ririn Dwi
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Health Professions Medicine & Pharmacology

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Antibacterial effect of ethanol extract of moringa oleifera seeds against enterococcus faecalis atcc 29212 Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.180-187

Abstract

Background: One of the important stages in endodontic treatment is root canal irrigation. The most widely used irrigant is sodium hypochlorite (NaOCl), but it is toxic to periapical tissue. Irrigant solutions from natural ingredients that have antibacterial potential are needed, one of which is Moringa oleifera seeds. Bacteria that are often found persistent after root canal treatment are Enterococcus faecalis. The purpose of this study was to determine the antibacterial effect of the ethanol extract of M. oleifera seeds at concentrations of 37.5%, 50%, 67.5% and 75% against E. faecalis.Method: Experimental laboratory research with post-test only control group design was carried out with 4 times replication. Antibacterial activity was tested against E. faecalis ATCC 29212 using paper disc diffusion method on Mueller Hinton Agar (MHA) media. The diameter of the inhibition zone formed was measured. Data were analysed by one-way ANOVA followed by LSD.Result: The 2.5% NaOCl group produced the largest inhibition zone of 16.38 ± 0.95 mm, followed by the 75% extract group of 13.51 ± 0.49 mm, and the smallest was the 37.5% extract group of 4.42 ± 0.27 mm. The negative control (DMSO) did not produce an inhibition zone. The increase in the concentration of the ethanolic extract of M. oleifera seeds resulted in significantly better inhibiting the growth of E. faecalis (p<0.05).Conclusion: Ethanol extract of M. oleifera seeds at 37.5%, 50%, 62.5% and 75% had antibacterial effects against E. faecalis, with the 75% had the strongest antibacterial effect compared to other extract concentrations. 
Antibacterial effect of ethanol extract of moringa oleifera seeds against enterococcus faecalis atcc 29212 Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.180-187

Abstract

Background: One of the important stages in endodontic treatment is root canal irrigation. The most widely used irrigant is sodium hypochlorite (NaOCl), but it is toxic to periapical tissue. Irrigant solutions from natural ingredients that have antibacterial potential are needed, one of which is Moringa oleifera seeds. Bacteria that are often found persistent after root canal treatment are Enterococcus faecalis. The purpose of this study was to determine the antibacterial effect of the ethanol extract of M. oleifera seeds at concentrations of 37.5%, 50%, 67.5% and 75% against E. faecalis.Method: Experimental laboratory research with post-test only control group design was carried out with 4 times replication. Antibacterial activity was tested against E. faecalis ATCC 29212 using paper disc diffusion method on Mueller Hinton Agar (MHA) media. The diameter of the inhibition zone formed was measured. Data were analysed by one-way ANOVA followed by LSD.Result: The 2.5% NaOCl group produced the largest inhibition zone of 16.38 ± 0.95 mm, followed by the 75% extract group of 13.51 ± 0.49 mm, and the smallest was the 37.5% extract group of 4.42 ± 0.27 mm. The negative control (DMSO) did not produce an inhibition zone. The increase in the concentration of the ethanolic extract of M. oleifera seeds resulted in significantly better inhibiting the growth of E. faecalis (p<0.05).Conclusion: Ethanol extract of M. oleifera seeds at 37.5%, 50%, 62.5% and 75% had antibacterial effects against E. faecalis, with the 75% had the strongest antibacterial effect compared to other extract concentrations. 
Anxiolytic effect of aqueous extract of moringa oleifera in balb/c mice Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.1-6

Abstract

Background: Anxiety control is required to achieve successful dental treatment especially in children. Dental anxiety is exaggerated fear or worry associated with dental treatment and it is followed with a sense of losing control. Moringa oleifera has been claimed as a potential plant to treat anxiety. A large amount of flavonoid in Moringa oleifera leaves is known to have anxiolytic effect due to their interaction with gamma-aminobutyricacid (GABA) receptors like benzodiazepine molecule. The aim of this study was to determine the anxiolytic effect of aqueous extract of Moringa oleifera (AEMO) leaves in balb/c mice. Method: Subject of this study was 25 mice divided into 5 groups. Diazepam as a positive control (K+), CMC-Na 0.5% as a negative control (K-), aqueous extract of M. oleifera leaves 375 mg/kgBW (P1), 500 mg/kgBW (P2), and 625 mg/kgBW (P3). Tests were conducted using Elevated Plus Maze. Before and after data were analyzed with paired T-test, differences of anxiety score between groups was analyzed with One-way ANOVA. Result: Group P2 and P3 significantly reduce anxiety in mice (p
Anxiolytic effect of aqueous extract of moringa oleifera in balb/c mice Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.1-6

Abstract

Background: Anxiety control is required to achieve successful dental treatment especially in children. Dental anxiety is exaggerated fear or worry associated with dental treatment and it is followed with a sense of losing control. Moringa oleifera has been claimed as a potential plant to treat anxiety. A large amount of flavonoid in Moringa oleifera leaves is known to have anxiolytic effect due to their interaction with gamma-aminobutyricacid (GABA) receptors like benzodiazepine molecule. The aim of this study was to determine the anxiolytic effect of aqueous extract of Moringa oleifera (AEMO) leaves in balb/c mice. Method: Subject of this study was 25 mice divided into 5 groups. Diazepam as a positive control (K+), CMC-Na 0.5% as a negative control (K-), aqueous extract of M. oleifera leaves 375 mg/kgBW (P1), 500 mg/kgBW (P2), and 625 mg/kgBW (P3). Tests were conducted using Elevated Plus Maze. Before and after data were analyzed with paired T-test, differences of anxiety score between groups was analyzed with One-way ANOVA. Result: Group P2 and P3 significantly reduce anxiety in mice (p
Co-Authors Rinaldi Budi Utomo Sri Kuswandari, Sri Utomo, Rinaldi