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All Journal Innovative: Journal Of Social Science Research
Tri Ambarwanto, Sigit
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Uji Kandungan Fenol Dan Flavonoid Ekstrak Daun Seligi (Phyllanthus Buxifolius) Dengan Pelarut Etanol Dan Etil Asetat Sunarmi, Sunarmi; Suhendriyo, Suhendriyo; Tri Ambarwanto, Sigit
Innovative: Journal Of Social Science Research Vol. 4 No. 6 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i6.16840

Abstract

Seligi leaves are traditionally used to treat sprains and reduce joint pain. The content of phenolic and flavonoid compounds in Seligi leaves also plays a role in its anti-inflammatory activity. Generally, phenolic and flavonoid compounds are soluble in polar and semi-polar solvents. This study aims to determine the levels of phenolic compounds and flavonoids in Seligi leaf extract. Seligi leaf simplicia was powdered with a fineness of 80 mesh and then extracted by maceration method using 96% ethanol and ethyl acetate. Each extract was tested for phenolic content quantitatively using the Folin-ciocalteu reagent with gallic acid as comparison, and the final result was expressed in terms of gallic acid equivalent by spectrophotometry at a wavelength of 731.5 nm. Determination of flavonoid levels using the UV-Vis spectrophotometry method with quercetin as a comparison at a wavelength of 435 nm. The results showed that the total phenolic content of the ethanol extract was 237.0 ± 9.4% mg GAE/g and that of the ethyl acetate extract was 12.8 ± 7.7% mg, GAE/g . Flavonoid content of ethanol and ethyl acetate extracts were 30.4 ± 9.5% mg QE/g and 10.5 ± 9.1% mg QE/g, respectively. Based on this, the content of phenol and flavonoid compounds was higher in extracts with ethanol solvent than ethyl acetate solvent.
Co-Authors Suhendriyo Suhendriyo Sunarmi Sunarmi