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All Journal Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Fullerene Journal of Chemistry
Nomi, Anastasia Grandivoriana
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Agriculture, Biological Sciences & Forestry Chemistry Engineering

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Effectiveness of Lontar Sap Vinegar and Lime Juice as a Substitute for HCl in the Production of Skipjack Fish Bone Gelatin Bora, Naema; Nomi, Anastasia Grandivoriana
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.560-569

Abstract

Gelatin is a product of collagen hydrolysis that is widely used in the food, pharmaceutical, and cosmetic industries. The gelatin production process generally uses hydrochloric acid (HCl) as a hydrolysis agent, but its use raises concerns about the environment and food safety. This study aims to evaluate the effectiveness of palm sap vinegar and lime juice extract as a substitute for HCl in the production of gelatin from skipjack fish bones (Katsuwonus pelamis). The study used a 3 x 3 factorial design with two main factors, namely the type of acid compound (HCl, coconut sap vinegar, and lime juice extract) and the acid concentration (5%, 7%, and 9%). The variables observed included yield, pH, moisture content, ash content, fat content, and gelatin protein content. The results showed that the type of acid and the con-centration of acid significantly affected the quality of gelatin. Palm sap vinegar produces gelatin with characteristics almost equivalent to HCl, with the highest protein content of 53.77%. Meanwhile, lime juice extract produced lower protein content (48.87%) yet provided lower fat content. The 7% and 9% acid concentration produces gelatin of the highest quality in terms of yield and protein content. This study shows that palm sap vinegar can be environmentally friendly and effective alternatives to replace HCl in gelatin production, with some adjustments to their concentration and application. Keywords: Acid concentration, Gelatin skipjack fish bones, Lime juice extract, Palm sap vinegar.
Isolation and Characterization of Dryland Microfungi From Northern Insana as Potential Sources of Antibacterial Agents mere, janrigo klaumegio; Edi, Eduardus; Nomi, Anastasia Grandivoriana
Jurnal Kimia Fullerene Vol 11 No 1: Fullerene Journal Of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v11i1.762

Abstract

Microfungi are one of the most extensively studied groups of microorganisms due to their remarkable ability to produce a diverse range of unique bioactive compounds. This study aimed to explore and characterize microfungi isolated from the drylands of North Insana as potential sources of sustainable antibacterial agents. The research methods included the isolation of microfungi, morphological identification, and evaluation of antibacterial activity using the disc diffusion method. Based on morphological characterization, microfungi isolated from the North Insana dry soil showed similarities to three genera, namely Mucor, Penicillium/Aspergillus, and Aspergillus. Antibacterial assays revealed that all three isolates exhibited varying degrees of activity, ranging from moderate to strong, against Staphylococcus aureus. Isolate 1 produced an inhibition zone of 7.6 ± 0.20 mm (moderate activity), isolate 2 produced 10.17 ± 0.06 mm (strong activity), and isolate 3 produced 10.77 ± 0.06 mm (strong activity). According to the Tukey test (p < 0.05), isolate 3 showed no significant difference from the standard antibiotic chloramphenicol (11.23 ± 0.15 mm), indicating a comparable antibacterial effectiveness. These results suggest that dryland-endemic microfungi possess adaptive biosynthetic capacities, enabling them to produce antibacterial secondary metabolites in response to extreme environmental stress. This potential reinforces the role of dryland microfungi as promising new sources of natural antibacterial compounds for sustainable biotechnological and health applications
Co-Authors Edi, Eduardus Mere, Janrigo Klaumegio Naema Bora, Naema