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All Journal Jurnal Ilmiah Pertanian Caraka Tani: Journal of Sustainable Agriculture
Irbati, Adenisa Hanifah
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Agriculture, Biological Sciences & Forestry

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Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia Santosa, Adyatma Irawan; Irbati, Adenisa Hanifah; Pratiwi, Geby Nandita Cahyaning; Surwadinata, Antama; Laeshita, Putri; Dharma, Krisnanda Surya; Jaya, Reko Saputra; Andriyani, Annisa Lisa; A'yun, Chilya Qurrota; Fatika, Chusnu Naura Syifa
Jurnal Ilmiah Pertanian Vol. 21 No. 1 (2024): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v21i1.18856

Abstract

Chili pepper, tomato, and squash cultivated in Magelang Regency, Central Java Province, Indonesia showed severe viral symptoms. Three samples were taken then molecularly tested against begomovirus, potyvirus, tobamovirus, and polerovirus. However, PCR test confirmed only begomovirus infections. BLAST analysis concluded that the chili pepper and tomato isolates were pepper yellow leaf curl Indonesia virus (PepYLCIV) while the squash isolate was squash leaf curl China virus (SLCCNV). The 552 bp partial AV1 gene sequences of the three isolates were given NCBI GenBank acc nos. OR924278-80. PepYLCIV OR924279 and OR924280 formed a subgroup with LC542629 from Bali in the phylogenetic tree constructed using MEGA11, and shared 95.8 – 96.7% identities at nucleotide (nt) and 98.4 – 99.5% at amino acid (aa) levels according to Sequence Demarcation Tool v1.2 software. Meanwhile, SLCCNV OR924278 was clustered, and shared 95.8 – 96.7% nt and 98.4 – 99.5% aa identities with three Malaysian isolates (MW248685, MW248687, and MW248689). Different plant species inoculated with SLCCNV OR924278 remained symptomless up to four weeks observation, suggesting that the isolate is not mechanically transmissible. This study contributed additional knowledge on molecular variation while expanding hosts and distribution of PepYLCIV and SLCCNV in Central Java Province.
Potato Virus Y and Shallot Latent Virus of Kajoran Horticultural Production Center, Magelang Regency, Indonesia: Molecular Characterization Case Study Santosa, Adyatma Irawan; Irbati, Adenisa Hanifah; Dharma, Krisnanda Surya; Winona, Belinda; Jaya, Reko Saputra; Andriyani, Annisa Lisa; A'yun, Chilya Qurrota
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 1 (2024): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i1.76737

Abstract

Kajoran Sub-district is a horticultural production center that supplies horticultural commodities for Central Java Province, especially Magelang. However, plant viral diseases of Kajoran have not been studied. This paper aimed to identify plant viral diseases in this horticultural production center. Some samples were taken: three potatoes (Solanum tuberosum) and three shallots (Allium cepa var. aggregatum), then tested respectively with RT-PCR, using two pairs of universal primers to detect Potyvirus and Carlavirus. The result showed two potato samples were infected by Potyvirus (potato virus Y/PVY) and three shallot samples by Carlavirus (shallot latent virus/SLV). GenBank accession no. OR180052 to OR180056 were assigned to the obtained partial sequences of the five isolates. The phylogenetic analysis makes the two new PVY Indonesian isolates in strain N group thus confirmed the presence of the first PVYN in Indonesia. The constructed SLV phylogenetic tree clusters the new three isolates with eight shallot isolates in a group and separated it from isolates from other plant species. The tested PVY isolates shared 95.2 to 100% nucleotide identities among them, while 78.9 to 98.5% identities were estimated among compared SLV isolates. This study expands our understanding of the genetic variation of PVY and SLV in Indonesia, potentially leading us to find the perfect management for the viruses.
Co-Authors A'yun, Chilya Qurrota Adyatma Irawan Santosa Andriyani, Annisa Lisa Dharma, Krisnanda Surya Fatika, Chusnu Naura Syifa Jaya, Reko Saputra Pratiwi, Geby Nandita Cahyaning Putri Laeshita, Putri Surwadinata, Antama Winona, Belinda