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The Effectiveness of Project-Based Learning Model with RAHMA Syntax to Improve Prospective Biology Teachers’ Critical Thinking Skills Darussyamsu, Rahmawati; Lufri, Lufri; Ahda, Yuni; Alberida, Heffi; Ambiyar, Ambiyar; Samsudin, Syakirah
Jurnal Pendidikan IPA Indonesia Vol. 13 No. 4 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/xy8mr440

Abstract

This research aims to measure the effectiveness of the Project-Based Learning Model with RAHMA Syntax (PjBL-RAHMA) in improving prospective biology teachers’ critical thinking skills. RAHMA is an acronym for Recognizing Problems, Analyzing Problems, Handling Projects, Monitoring Progress, and Assessing Results. This research was quasi-experimental with a posttest-only control group design. This research involved 223 prospective biology teachers from three well-accredited public universities in Sumatra, Indonesia. Data from the critical thinking skills test results were analyzed using JASP software. Prospective biology teachers’ achievement of critical thinking skills after learning using the PjBL-RAHMA model showed that the experimental class was better than the control class. This research concludes that PjBL-RAHMA effectively improves prospective biology teachers’ critical thinking skills. This research also explains the contribution of each PjBL-RAHMA syntax in improving prospective biology teachers’ critical thinking skills. The PjBL-RAHMA syntax has increased prospective biology teachers’ ability to understand and analyze real-life problems, providing good reasons, logical thinking, and creating solutions to problems, especially in pedagogy.
Glutathione Transferase Response to Platinum Doped Titanium Dioxide in Aedes aegypti Pupae Al-Salih, Moatasem; Al-Salih , Moatasem; Samsudin, Syakirah; Misnan, Rosmilah bt
Indonesian Journal on Health Science and Medicine Vol. 2 No. 3 (2025): Desember
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v2i3.302

Abstract

General Background: Aedes aegypti is a major vector of arboviruses, and increasing resistance to chemical insecticides demands novel control strategies. Specific Background: Photocatalytic nanoparticles such as Pt-doped TiO₂ generate reactive oxygen species under light, inducing oxidative injury in mosquitoes; however, the biochemical defense response, particularly glutathione S-transferase (GST), remains insufficiently understood at the pupal stage. Knowledge Gap: No study has simultaneously assessed lipid peroxidation and GST activity in Ae. aegypti pupae exposed to Pt-TiO₂ under different light conditions. Aims: This research examined malondialdehyde (MDA) levels and GST activity in pupae exposed to Pure TiO₂ and Pt-TiO₂ under light and dark conditions. Results: Light exposure significantly amplified oxidative stress and GST activity, with Pt-TiO₂ producing the highest MDA and strongest GST induction, while dark conditions showed no meaningful biochemical alterations. Novelty: The study provides the first integrated evidence linking enhanced photocatalytic ROS generation by Pt-TiO₂ to concurrent oxidative damage and compensatory antioxidant activation in pupae. Implications: These findings clarify the mechanism of Pt-TiO₂ toxicity and support its use as an efficient light-activated nano-insecticide for vector control.Highlight : Highlights the strong light-dependent oxidative stress induced by Pt-doped TiO₂ nanoparticles. Emphasizes GST upregulation as a key biochemical response to nanoparticle exposure. Shows that lipid peroxidation remains elevated despite antioxidant activation. Keywords : Aedes aegypti; Pt-doped TiO₂ nanoparticles; oxidative stress; glutathione S-transferase; malondialdehyde