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Kusumah, R Yayi Munara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Genetic variation of pest fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in different landscapes in Bogor: Keragaman genetik hama ulat gerayak jagung Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) pada lanskap yang berbeda di Bogor Fahmi, Fajrin; Kusumah, R Yayi Munara; Buchori, Damayanti
Jurnal Entomologi Indonesia Vol 20 No 1 (2023): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.1.1

Abstract

Spodoptera frugiperda is an invasive pest from the American continent that attacks corn (Zea mays) and rapidly invaded Africa and Asia. Two main factors that support migration and population distribution of this species are suitable habitats and human activities. To date, two genetic strains of S. frugiperda have been found in corn in Indonesia: the corn strain (CS) and the rice strain (RS). The most accurate gene markers to detect these strains are COI and Tpi, which are located in mitochondria and Z chromosome. This study aims to determine the existing strains of S. frugiperda and their distribution in various landscapes in Bogor Regency. The research was conducted from July 2020 to December 2021 in Bogor, West Java. Sampling of S. fungiperda was carried out from corn plants in Leuwisadeng, Pamijahan1, Pamijahan2, Kemang, Tenjolaya, Dramaga, Cigombong, Cijeruk, Tamansari, and Ciomas. Larval samples were collected and preserved using 96% ethanol, followed by DNA extraction, DNA amplification, electrophoresis, and DNA sequencing. Distribution data were analyzedusing QGIS and Google Earth Pro programs, and statistical analysis was performed using SPSS 22. Sequence data were edited using GeneStudio, aligned using ClustalW in BioEdit, and the phylogeny tree was reconstructed using the neighbor-joining method (bootstrap 1000x) using MEGA X. The obtained sequences were compared with sequences from the GenBank® database. The results showed the presence of two distinct strains of COI (COI-CSh4 and COI-RS) and one strain of Tpi (Tpi-C) in Bogor. The study found no relationship between  thelandscape structure and genetic variation of S. frugiperda.
Analisis filogenetik Hyposidra talaca nucleopolyhedrovirus (HytaNPV) yang diisolasi dari perkebunan teh Gunung Mas, Bogor, Jawa Barat dan virulensinya terhadap Hyposidra talaca Walker Kusumah, R Yayi Munara; Kurniawati, Fitrianingrum; Kristanto, Eka Dana; Parasian, Franciskus; Christian, Michael
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.151

Abstract

Hyposidra talaca (Walker) is an important pest of tea plant. H. talaca can cause losses of between 40–100% in the dry season if proper control is not carried out. H. talaca has natural enemies such as predators, parasitoids, and pathogens. One of the entomopathogens is NPV. This study aims to obtain molecular characteristics through DNA polymerase sequences and determine the virulence level of NPV isolates from H. talaca. The same species from different locations can have genetic variability. Therefore, molecular characterization by polymerase chain reaction (PCR) on DNA polymerase sequences is one way to study the genetics of HytaNPV. NPV was isolated from infected H. talaca larvae collected from the field. The DNA isolates were used as templates for PCR for DNA polymerase gene amplification with an amplicon target of ±1,000 bp. A sequencing process followed the PCR provides nucleotide sequence. HytaNPV DNA polymerase sequencing results were aligned with GenBank's BLAST data to provide information on the relationship of HytaNPV to NPVs isolated from other regions. Based on molecular character analysis using DNA polymerase gene sequence, HytaNPV Bogor has a homology level of 93.9% with HytaNPV isolated from India. HytaNPV Bogor has a genetic relationship with the NPV that infects Buzura suppressaria from China and Australia. HytaNPV Bogor is similar to the NPV that infects H. talaca from India. The bioassay of HytaNPV isolate against H. talaca showed the highest LT50 value of 1.92 days was found in concentration of 1.58 x 107 POBs/ml in second instar larvae.
Co-Authors Christian, Michael DAMAYANTI BUCHORI Fahmi, Fajrin Fitrianingrum Kurniawati, Fitrianingrum Kristanto, Eka Dana Parasian, Franciskus