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PEMBUATAN BIOETANOL DARI EMPULUR DAN LIMBAH SERAT SAGU DENGAN METODE KIMIAWI DAN ENZIMATIS Ina Winarni; Totok K. Waluyo; Sri Komarayati
Jurnal Penelitian Hasil Hutan Vol. 37 No. 1 (2019): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2019.37.1.43-50

Abstract

Sago (Metroxylon spp.) is one of the native Indonesian plants that can be converted into biofuel or bioethanol. All parts of sago: starch, stem and midrib could be converted into bioethanol. The objective of this research is to determine ethanol production techniques made of pith and sago waste fiber using chemical and enzymatic methods. Bioethanol technique used was chemical based using hydrochloric acid solution (HCl) of 3.1; 6.2; 9.3 and 12.4% with substrate concentration of 15% (dw) and then heated by autoclave at 121–127 °C for 5, 10 and 15 minutes; pressure of 1–1.5 bar. The enzymatic method was undertaken using 0.15 ml alpha amylase enzyme and 2%, 3% and 4% cellulase. Fermentation was conducted using 0.21 g of urea addition, 0.12 g of NPK and 0.48 g of yeast for 4 days. Results showed that the pith yielded higher reducing sugar content and ethanol content than sago fiber waste either by chemical method (446.34 g/l, 51.65%) or enzymatic (15.39 g/l, 16.82%). Greater of cellulase concentration from 2% to 4% could not improve the yield of ethanol either made from pith or the wasted sago fiber through hydrolysis process.
PEMBUATAN BIOETANOL SECARA ENZIMATIS DARI LIMBAH BATANG SAWIT (Elaeis guineensis) DENGAN PENAMBAHAN SURFAKTAN Ina Winarni; Sri Komarayati; T. Beuna Bardant
Jurnal Penelitian Hasil Hutan Vol. 34 No. 2 (2016): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.2.127-135

Abstract

Biomass is an alternative potential raw materials for biofuels or bioethanol production. This paper studies bioethanol production of theoil palm trunk waste. The trunk was divided intothree parts; parenchym(P),vascular bundle (VB) and the mixtureof parenchyma and vascular bundle (PVB). Results show that sugar content reduction of all treatments (using surfactant) before fermentation is higher than that without surfactant (control) at 10 and 15 FPU/g substrateof cellulase concentration. The highest ethanol content (1.63%) was recorded from parenchyma with 15 FPU/g substrate of cellulase concentration.
FORMULASI RAGI CAMPURAN UNTUK PRODUKSI BIOETANOL DARI LIMBAH KAYU SENGON Ina Winarni; Sri Komarayati; Djarwanto
Jurnal Penelitian Hasil Hutan Vol. 35 No. 2 (2017): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2017.35.2.135-143

Abstract

Currently, bioethanol is one of biofuels which is potentially developed for new and renewable alternative energy. Bioethanol could be fermented from agriculture and forest wastes, with pre-treatment and hydrolysis process before the fermentation. Those process could be carried out by an addition of microbe (yeast). In order to gain greater ethanol content, yeast mixing formulation is crucial to obtain optimum ethanol content. This paper studies the formula of yeast mixing for optimum ethanol production of sengon wood waste. Formulation was conducted by mixing Aspergillus oryzae, Saccharomyces cerevisae, Rhyzopus oryzae and other substances with various treatments. Commercial yeast of 3 – 9% Sacharomyces cerevisae was used as a comparable control. Results showed that 7% yeast mixing was effective to produce 1.569% ethanol content from lignocellulose fermentation. The ethanol content is relatively greater than those of commercial yeast which constitutes of about 0.652% ethanol content.