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All Journal JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Lena, Wirda
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Isolation and Identification of Phenolic Compounds from Sea Grape (Coccoloba uvifera (L.) L) Pasaribu, Albert; Lena, Wirda
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11984

Abstract

Isolation and identification of phenolic compounds from Sea Grapes (Coccoloba uvifera (L.) L.) leave, where as much as 1700 g have been macerated using methanol. The concentrated methanol extract was dissolved in water and partitioned with ethyl acetate. Ethyl acetate concentrated extract was separated by column chromatography with chloroform as eluents: methanol 90:10; 80:20; 70:30; 60:40 v/v. The compound obtained was purified by column again using chloroform: ethyl acetate 70:30 v/v with the resulting was 10 mg of brownish yellow paste with a value of Rf = 0.5. The resulting compounds were then analyzed using a UV-Vis, FT-IR, and 1H-NMR spectrophotometer. Based on the data analysis, the results showed phenolic compounds in Sea Grape.
Co-Authors Pasaribu, Albert