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All Journal JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Simangunsong, Siska NA
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Journal : JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)

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Isolation of Flavonoids Compounds from Akalifa (Acalypha wilkesiana Muell. Arc.) Plant Leaves Simangunsong, Siska NA; Lenny, Sovia; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11990

Abstract

Flavonoid compounds from the leaves of Akalifa (Acalypha wilkesiana Muell. Arc.) have been extracted with maceration by methanol solvent, then added with ethyl acetate and partition extracted with n-hexane. 6% HCl acidifies the concentrated methanol extract, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with silica gel 40 as the stationary phase and n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/v) as the mobile phase. The compounds were purified with TLC preparative yielding tawny paste weighing 4 mg with Rf= 0.42 with eluent n-hexane: ethyl acetate 60:40 (v/v). The identified analysis by using Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance (1H-NMR) Spectroscopy was estimated as a flavonoid is an isoflavone.
Co-Authors Lamek Marpaung, Lamek Sovia Lenny