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All Journal KLOROFIL: Jurnal Ilmu Biologi dan Terapan KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Damayanti, Putri
Universitas Negeri Makassar
Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Analisis Cemaran Bakteri Coliform pada Jajanan Sekitar Kampus Universitas Negeri Makassar Damayanti, Putri
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.25490

Abstract

The safety of ready-to-eat street food around university campuses is a growing concern due to potential contamination by pathogenic microorganisms, especially coliform bacteria. This study aimed to analyze the microbiological quality of popular street foods consumed by students around Universitas Negeri Makassar using the Total Plate Count (TPC) and Most Probable Number (MPN) methods. Six food samples were collected purposively and tested in the Science Education Laboratory at Universitas Negeri Makassar in April 2025. The TPC method was applied to determine the total number of microorganisms, and the MPN method was used to estimate the presence of coliforms through presumptive, confirmed, and completed tests. TPC results showed that 4 out of 6 samples (66.66%) exceeded the maximum limit set by Indonesian National Standard (SNI 7388:2019), indicating potential food safety risks. The MPN test revealed that 5 of 6 samples tested positive for coliforms, with values ranging from 460 to >1100 MPN/gram, far above the acceptable limit of ≤3 MPN/gram. Confirmation tests using Eosin Methylene Blue Agar (EMBA) identified Escherichia coli through characteristic metallic green sheen colonies in 2 samples, and Enterobacter spp. in 3 samples with purple colonies. Gram staining results confirmed all isolates were Gram-negative rods, consistent with coliform characteristics. These findings indicate a significant risk of microbiological contamination in street foods commonly consumed around the university area. The presence of fecal indicator bacteria such as E. coli reflects poor hygiene in food handling and environmental sanitation. Strengthening food safety education among food vendors and regular monitoring by relevant authorities is essential to protect public health, especially in academic settings
Analisis Cemaran Bakteri Coliform pada Jajanan Sekitar Kampus Universitas Negeri Makassar Damayanti, Putri
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.25490

Abstract

The safety of ready-to-eat street food around university campuses is a growing concern due to potential contamination by pathogenic microorganisms, especially coliform bacteria. This study aimed to analyze the microbiological quality of popular street foods consumed by students around Universitas Negeri Makassar using the Total Plate Count (TPC) and Most Probable Number (MPN) methods. Six food samples were collected purposively and tested in the Science Education Laboratory at Universitas Negeri Makassar in April 2025. The TPC method was applied to determine the total number of microorganisms, and the MPN method was used to estimate the presence of coliforms through presumptive, confirmed, and completed tests. TPC results showed that 4 out of 6 samples (66.66%) exceeded the maximum limit set by Indonesian National Standard (SNI 7388:2019), indicating potential food safety risks. The MPN test revealed that 5 of 6 samples tested positive for coliforms, with values ranging from 460 to >1100 MPN/gram, far above the acceptable limit of ≤3 MPN/gram. Confirmation tests using Eosin Methylene Blue Agar (EMBA) identified Escherichia coli through characteristic metallic green sheen colonies in 2 samples, and Enterobacter spp. in 3 samples with purple colonies. Gram staining results confirmed all isolates were Gram-negative rods, consistent with coliform characteristics. These findings indicate a significant risk of microbiological contamination in street foods commonly consumed around the university area. The presence of fecal indicator bacteria such as E. coli reflects poor hygiene in food handling and environmental sanitation. Strengthening food safety education among food vendors and regular monitoring by relevant authorities is essential to protect public health, especially in academic settings
Pengaruh Lama Fermentasi Biofertilizer terhadap Pertumbuhan Tinggi Batang Tanaman Kangkung (Ipomoea reptans) dalam Sistem Hidroponik Muhiddin, Nurhayani H.; Syakur, akhmad; damayanti, putri; U, sahrani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.26302

Abstract

This study aims to determine the effect of biofertilizer fermentation duration on the stem length growth of water spinach (Ipomoea reptans) in a hydroponic system. The biofertilizer was fermented for 7, 14, and 21 days, then combined with AB mix nutrient solution in various concentrations. The experimental design used was a Randomized Block Design (RBD) with five treatments: 100% AB mix control, biofertilizer combined with 75%, 50%, and 25% AB mix, and biofertilizer without AB mix, with three replicates for each fermentation duration. The parameter observed was the stem length of water spinach plants after five weeks of cultivation. The results showed that the biofertilizer from the 21-day fermentation had the most significant effect on stem length increase, particularly in combination with 50–75% AB mix. Meanwhile, the use of biofertilizer without AB mix resulted in shorter stem lengths. Thus, the biofertilizer from the 21-day fermentation process has potential as a nutritional supplement in hydroponic systems, but it cannot fully replace the role of AB mix.
Pengaruh Lama Fermentasi Biofertilizer terhadap Pertumbuhan Tinggi Batang Tanaman Kangkung (Ipomoea reptans) dalam Sistem Hidroponik Muhiddin, Nurhayani H.; Syakur, akhmad; damayanti, putri; U, sahrani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 1 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i1.26302

Abstract

This study aims to determine the effect of biofertilizer fermentation duration on the stem length growth of water spinach (Ipomoea reptans) in a hydroponic system. The biofertilizer was fermented for 7, 14, and 21 days, then combined with AB mix nutrient solution in various concentrations. The experimental design used was a Randomized Block Design (RBD) with five treatments: 100% AB mix control, biofertilizer combined with 75%, 50%, and 25% AB mix, and biofertilizer without AB mix, with three replicates for each fermentation duration. The parameter observed was the stem length of water spinach plants after five weeks of cultivation. The results showed that the biofertilizer from the 21-day fermentation had the most significant effect on stem length increase, particularly in combination with 50–75% AB mix. Meanwhile, the use of biofertilizer without AB mix resulted in shorter stem lengths. Thus, the biofertilizer from the 21-day fermentation process has potential as a nutritional supplement in hydroponic systems, but it cannot fully replace the role of AB mix.
Co-Authors Akhmad Syakur Muhiddin, Nurhayani H. U, sahrani