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All Journal Jurnal Ilmiah Biologi UMA (JIBIOMA) Journal of BioSustainability
Marhamah, Yualinda Durotul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Kandungan Metabolit pada Planlet Tebu (Saccharum officinarum L.) pada Perbedaan Media dan Frekuensi Subkultur Marhamah, Yualinda Durotul; Kusdianti, Kusdianti; Surakusumah, Wahyu
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 7, No 2 (2025): November 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v7i2.6509

Abstract

Sugarcane has been widely used to treat various diseases due to its metabolite content. Tissue culture is one method that can be used to produce metabolites and is an alternative biotechnology as a conservation strategy. This study aims to analyze the differences in metabolite content in sugarcane plantlets cultured with different media and subculture frequencies. Plantlets were extracted using the maceration method with 70% p.a. ethanol solvent. Metabolite analysis was carried out using a Gas Chromatography-Mass Spectrometry (GC-MS) instrument. Metabolite identification was carried out based on the NIST library. The results showed that the 10th subculture of sugarcane plantlets cultured on MS+Kinetin+Coconut Water media contained 5 metabolites, while the 11th subculture of plantlets cultured on MS+Kinetin+Tidiazuron media contained 7 metabolites. Differences in the type and number of metabolites indicate the influence of culture conditions on the metabolite profile of the plantlets. This study provides an initial overview of the potential of sugarcane tissue culture in the production of secondary metabolites.
The Effect of Banana Peel Biosorbent (Musa Acuminate) on Water Clarity, pH, TDS, and DO Levels in Household Wastewater in Bandung Fatharani, Aulia; Al-Wafi, Ok M Abthal; Marhamah, Yualinda Durotul; Shani, Nadiya Syafia; Fitriani, Any; Yulianty, Nurfitri
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

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Abstract

Banana peel consists of nitrogen atoms, sulfur and organic materials such as carboxylic acids that can function to bind pollutant molecules. The purpose of this research is to make King Banana (Musa acuminate) peel as filtration media to improve the physical quality of water including water clarity, pH value, TDS and Dissolved Oxygen (DO). There are three stages carried out, including preparation of banana peel waste, adjusting the media and filtration time and data analysis. The preparation process of banana peel waste is done by cleaning the banana peel then dried and mashed, then alkalized using 0.5 N NaOH. Then a variation in the amount of filtration media is made, namely: 60 g, 80 g and 100 g of plantain peels formulated using an additional 30 grams of zeolite with variations in filtration time, namely: 3 hours, 6 hours and 9 hours. The filtration results obtained using a variation of 100 grams of filtration media with a filtration time of 9 hours is the best media combination where the results obtained show an increase in water clarity, pH 5.41 and an increase in DO (Dissolve oxygen) of 20.
Co-Authors Al-Wafi, Ok M Abthal Any Fitriani Fatharani, Aulia Kusdianti Kusdianti Shani, Nadiya Syafia Wahyu Surakusumah Yulianty, Nurfitri