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Kusdianti Kusdianti
Prodi Biologi, Jurusan Pendidikan Biologi, FPMIPA UPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Education Immunology & microbiology Mathematics Medicine & Pharmacology

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Analisis Pertumbuhan Tanaman Kentang (Solanum tuberosum L) pada Tanah yang Terakumulasi Logam Berat Cadmium (Cd) (Growth Analysis of Potato (Solanum tuberosum L.) in Accumulates Of Heavy Metal Cadmium(Cd) Soil) Kusdianti, Kusdianti; Solihat, Rini; Hafsah, Hafsah; Trisnawati, Eva
JURNAL BIOS LOGOS Vol 4, No 1 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.1.2014.4839

Abstract

Abstrak Pupuk dan pestisida kimiawi merupakan hal penting pada sistem budidaya tetapi jika  penggunaannya berlebih dapat meningkatkan kandungan logam berat kadmium  (Cd) dalam tanah. Penelitian ini bertujuan menganalisis pertumbuhan tanaman kentang (Solanum  tuberosum L.) pada tanah yang terakumulasi logam berat kadmium. Sampel tanaman berasal dari lokasi pertanian kentang Pangalengan Jawa Barat. Parameter yang diukur yaitu kandungan klorofil, biomassa, kandungan logam kadmium dalam tanah dan umbi kentang. Kandungan kadmium dalam sampel tanah dan umbi kentang diukur dengan menggunakan Atomic Absorption Spectrophotometry (AAS). Hasil penelitian menunjukkan kandungan kadmiumpada lahan pertanian kentang telah melebihi ambang batas. Kadmium terakumulasi dalam umbi kentang, tetapi masih berada di bawah ambang batas. Klorofil, berat kering, dan berat basah mengalami kenaikan setiap minggunya (4-10 Minggu Setelah Tanam). Adanya logam kadmium  yang diserap oleh tanaman kentang dapat menghambat pembentukan  klorofil sehingga akan mempengaruhi biomassa tanaman. Kata kunci: klorofil, biomassa, kentang, kadmium Abstract Chemical fertilizers and pesticides are important in the culture system but if the excessive usage may increase the content of heavy metals cadmium ( Cd ) in the soil. This study aims to analyze the growth of the potato ( Solanum tuberosum L.) on the ground that accumulate heavy metals cadmium. Plant samples derived from potato farming locations Pangalengan West Java.The parameters are chlorophyll content, biomass, cadmium  content in soil and potato tubers. The content of cadmium in soil and potato tuber samples were measured using Atomic Absorption Spectrophotometry (AAS). The results showed cadmium content in potato farms has exceeded the threshold. Cadmium accumulates in potato tubers, but still below the threshold. Chlorophyll, dry and fresh weight increase every week (4-10 Weeks After Planting). The presence of the metal cadmium is absorbed by the potato can inhibit the formation of chlorophyll that will affect plant biomass . Keywords: chlorophyll, biomass, potatoes, cadmium
Potensi Antioksidan Alami pada Ekstrak Kulit Buah Jamblang (Syzigium cumini (L.) Skeels) Menggunakan Metode DPPH (The Potency of Natural Antioxidant in The Rind Extract of Jamblang (Syzigium cumini (L.) Skeels) using DPPH Method) Sari, Ayu Nirmala; Kusdianti, Kusdianti; Diningrat, Diky Setya
JURNAL BIOS LOGOS Vol 8, No 1 (2018): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.8.1.2018.20593

Abstract

AbstrakStress oksidatif pada tubuh dapat memicu berbagai penyakit. Stress oksidatif ini disebabkan karena radikal bebas berlebih. Tubuh memerlukan antioksidan untuk mengurangi pengaruh radikal bebas dan meredam dampak negatifnya. Penelitian ini bertujuan untuk mengevaluasi daya antioksidan ekstrak kulit buah jamblang (Syzigium cumini (L.) Skeels) dengan menggunakan metode DPPH. Berdasarkan hasil pengujian daya antioksidan ekstrak kulit buah jamblang diketahui bahwa ekstrak kulit buah jamblang memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC50 sebesar 169.3. Hasil penelitian ini menunjukkan bahwa kulit buah jamblang berpotensi untuk dikembangkan sebagai sumber antioksidan alami bagi manusia.Kata Kunci : antioksidan, DPPH, ekstraksi, jamblang, radikal bebas, stress oksidatif AbstractOxidative stress can induce many diseases. Oxidative stress is caused by excessive free radicals in the body. The body required antioxidant to decrease and hush the negative effects of free radicals. The aim of this study was to evaluate the antioxidant potency of extract rind of jamblang (Syzigium cumini (L.) Skeels) using DPPH method. The result showed that the jamblang rind extract had moderate antioxidant activity and the IC50 value was 169.3.  Based on this result, jamblang rind was potential to be natural antioxidant for human.Keyword: antioxidant, DPPH, extraction, jamblang, free radicals, oxidative stress
Hubungan Kekerabatan Kultivar Talas (Colocasia esculenta) Berdasarkan Karakter Morfologi Organ Vegetatif (The Phenetic Relationship among Taro Cultivar (Colocasia esculenta) Based on Vegetative Morphological Characters) Hafsah, Hafsah; Hidayat, Topik; Kusdianti, Kusdianti
JURNAL BIOS LOGOS Vol 4, No 1 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.1.2014.4838

Abstract

Abstrak   Telah dilakukan penelitian mengenai hubungan kekerabatan pada delapan kultivar talas (Colocasia esculenta) berdasarkan karakter morfologi organ vegetatif. Kultivar-kultivar tersebut yakni talas kaliurang, burkok, bentul, bogor, sutra, kudo, lampung dan semir. Total karakter yang diamati berjumlah 35 karakter. Karakter-karakter tersebut kemudian dibuat skoring. Hasil skoring dianalisis dengan metode UPGMA (Unwight Pair Group Method with Arithmetic Average) menggunakan program komputer PAUP versi 4.0b10 sehingga terbentuk fenogram. Hasil penelitian menunjukkan variasi morfologi organ vegetatif yang cukup tinggi. Dari seluruh karakter, hanya lima karakter yang tidak menunjukkan adanya perbedaan. Karakter tersebut yaitu bentuk daun dan ujung daun, rasio panjang terhadap lebar daun, pola tepi pelepah dan posisi dominan bentuk helai daun tua. Dari fenogram diketahui bahwa seluruh kultivar yang diamati membentuk dua kelompok utama. Kelompok pertama terdiri dari kaliurang, burkok, bentul, bogor, lampung dan sutra, yang membentuk tiga subkelompok. Kelompok kedua terdiri dari kudo dan semir. Hasil analisis fenetik menunjukkan kultivar bogor dan lampung merupakan dua kultivar yang sangat dekat hubungan kekerabatannya dengan nilai kesamaan sebesar 80%. Kata kunci : Colocasia esculenta, fenetik, kultivar, organ vegetatif Abstract This research was conducted to find out relationship on eight cultivars of taro (Colocasia esculanta) based on vegetative morphological characters. They were kaliurang, burkok, bentul, bogor, sutra, kudo, lampung and semir. Total characters observed were 35 characters and the scoring  was also performed. In order to form a phenogram, the scoring then were analyzed using computer software PAUP version 4.0b10, the UPGMA method (Unwight Pair Group Method with Arithmetic Average). Results showed that morphological variation of vegetative characters are quite high. Of all the characters, only five characters that indicated any significant differences. These characters were leaves shape, leaves tip shape, length-width leaves ratio, midrib leaves, and dominant position of the old leaves. From the phenogram, it was known that all cultivars observed were form into two main groups. The first group consists of kaliurang, burkok, bentul, bogor, lampung and sutra, which formed three subgroups. The second group consists of kudo and semir. The results of the phenetic analysis showed the cultivar bogor and lampung are two cultivars that are very close relationship ties with the similarity value of 80%. Keywords : Colocasia esculenta , phenetic, cultivars, vegetative
PENGARUH 6-BENZILAMINOPURIN (BAP) TERHADAP PEMBENTUKAN LAPISAN PEMISAH (ZONA ABSISI) PADA TANGKAI KUNTUM BUNGA KACANG HIJAU, VIGNA RADIATA (L.) Wilczek VARITAS WALET Kusdianti, Kusdianti; Wardini, Trimurti H
Jurnal Pengajaran MIPA Vol 3, No 1 (2002): JPMIPA: Volume 3, Issue 1, 2002
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v3i1.34967

Abstract

The effect of 6-benzylaminopurine (BAP) on separation layer formation of mungbean Vigna radiata (L.) Wilczek var. walet was carried out. The objective was to know the effect of BAP on separation layer formation. The experiment was done by spraying the first inflorescent with 8 x 10-4 M BAP three days before and three days after the fifth flower was anthesis.  Samples of flower pedicellus were taken three days before dan three days after fifth flower was bloom. Then they were  fixed and processed for anatomical observation by preparing histological slides accoding to O’Brien and Horner (1981). The result show that in control plant, two days before the fifth flower started to anthesis, cells of the abscission zone started to be activated to form separation layer. It was initiated by cell division within the adaxial edge and progressing inward across the cortex to the vascular strands. Abscission layer was completed at the time or a day after the fifth flower was anthesis. No histological changes observed in with 8 x 10-4 M BAP treated plant. No abscission layer were recorded for the pedicels treated plant during the course of the experiment.
Rekonstruksi LKPD untuk meningkatkan keterlibatan dan penguasaan konsep peserta didik pada materi sistem ekskresi Rahmadatillah, Fauziani; Riandi, Riandi; Kusdianti, Kusdianti
Assimilation: Indonesian Journal of Biology Education Vol 4, No 2 (2021): September 2021
Publisher : Department of Biology Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/aijbe.v4i2.41491

Abstract

This research was aimed at investigating the information about the use of the Student Worksheet (LKPD) the results of reconstruction about the involvement and mastery of students' concepts in the material of human excretion system. Reconstruction of student worksheets was done because the previous research shows analysis results the component of diagram vee in the eksperiment  students worksheet is low. Whereas, the diagram Vee's rubric is usually used for developing eksperiment  students worksheet. This research is a descriptive research. The subject of the research are 28 students of grade XI IPA in senior high school Bandung. The instrument that used is the rubric of component of diagram Vee's existence, observation worksheet in students engagement and test of mastering concept that consist is multiple choice. The result of the research shows : (1) students engagement in the class is about 79,17%; (2) students' mastery of concepts based on categories is good, 96.43% of students achieve maximum completeness criteria (KKM), and an average N-gain of 0.72. This showed the influence of use reconstruction  of student worksheets on the student engagement and mastery of student concepts.
Metabolite Profile of arabica Coffee Cascara from Typica Cultivar in Bandung with Different Drying Processes Sururi, Zaki Fahreza; Diana, Sariwulan; Kusdianti, Kusdianti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.52756

Abstract

Arabica coffee bean (Coffea arabica L.) is Indonesia's leading commodity, with Bandung Regency as the largest producer in West Java. The exocarp and mesocarp of coffee fruits known as cascara have potential health benefits as a beverage. Cascara is usually dried by direct sunlight before consumption, but this process is prone to weather disturbances and contamination. The use of dehydrators is more controlled to maintain metabolite content, but there has been no research on cascara from coffee cultivars in Bandung Regency dried by this method. This study aims to obtain the metabolite profile of Typica arabica coffee cultivar cascara dried using direct sun and dehydrator. The cascara samples were taken from Gunung Puntang Coffee Plantation, Bandung Regency, and extracted using maceration method with 70% ethanol p.a. solvent. The metabolite content was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS), which specifically detects volatile metabolites. Non-volatile compounds were not within the scope of this study. The results showed that direct sun-dried cascara had 6 metabolites, while dehydrator-dried had 15 metabolites. Both samples were dominated by caffeine (sun-dried cascara: 46.61%, dehydrator cascara: 24.44%). Five metabolites were found in both samples, with 1 unique metabolite in solar cascara and 10 unique metabolites in dehydrator cascara. This study showed differences in metabolite content in Typica cultivar cascara with different drying methods.
Phytochemical Analysis, Antioxidant Activity, and Cytotoxic Effects of Physalis angulata and Moringa oleifera Leaf Extracts on Cancer and Normal Cells In Vitro Priyandoko, Didik; Widowati, Wahyu; Sholihah, Ika Adhani; Zahira, Nurul Zahra; Bangun, Adityana Arta; Kusdianti, Kusdianti; Hernawati, Hernawati; Surakusumah, Wahyu; Juansah, Rahadian Deden
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Physalis angulata and Moringa oleifera have long been the key ingredients of traditional medicine. This study aimed to obtain the metabolite profiles of the ethanolic leaf extracts of P. angulata (ELP) and M. oleifera (ELM), evaluate their antioxidant activity, and assess their toxicity against normal and cancer cells. The leaves were sourced from Balittro, West Java. The extraction used 70% ethanol and continued for 3 days. The metabolites were identified using Gas Chromatography-Mass Spectrometry (GC-MS), antioxidant activity was ascertained via the DPPH assay, and cytotoxicity was evaluated employing the PrestoBlue assay. GC-MS identified 23 compounds in ELP and 15 in ELM. The DPPH assay showed that ELM, ELP, or their combination exhibited marked antioxidant activity, with inhibition ranging from 50% to 90%. The toxicity assay against MCF-7 cancer cells demonstrated that ELP at 1,500 µg/mL and ELM at 1,200 µg/mL induced cytotoxicity. The ELP + ELM formulation exhibited stronger cytotoxic effects than the positive control. In contrast, ELM or ELP did not induce toxicity in normal CV-1 cells and, stimulated viability com-pared to the control. Thus, the phytochemical contents of ELM and ELP exhibited enhanced antioxidant activity, in-duced toxicity in cancer cells, and were safe for normal cells.
Analisis Potensi Senyawa Bioaktif Minyak Esensial Akar dan Biji Hanjeli (Coix lacryma-jobi L.) sebagai Antivirus SARS-CoV-2 Secara In Silico Sinaga, Acong Jaya; Diningrat, Diky Setya; Sari, Ayu Nirmala; Harahap, Novita Sari; Kusdianti, Kusdianti
Kalwedo Sains (KASA) Vol 4 No 1 (2023): Kalwedo Sains (KASA), Maret 2023
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav4i1p1-16

Abstract

Pandemi Covid-19 masih menjadi masalah yang serius, penyebaran virus dan mutasi virus yang sangat cepat menyebabkan penemuan obat yang spesifik masih kurang efektif. Penelitian ini bertujuan untuk mengetahui profil senyawa bioaktif yang terkandung dalam minyak esensial akar dan biji hanjeli, khususnya senyawa yang bermanfaat sebagai antivirus. Jenis dari penelitian ini adalah penelitan deskriptif dengan metode In silico. Akar dan biji hanjeli diolah dengan cara destilasi uap air. Setelah itu identifikasi senyawa bioaktif dilakukan menggunakan teknik GC-MS, untuk mengetahui senyawa bioaktif yang terkandung pada ekstrak minyak esensial akar dan biji Hanjeli. Selanjutnya hasil senyawa bioaktif yang telah didapat kemudian diidentifikasi bioaktivitasnya dengan menggunakan software Pubchem, untuk melihat bioaktivitas dan canonical SMILES dari senyawa tersebut dan terakhir dilakukan analisis dengan PASS online untuk melihat potensi dan mekanismenya sebagai antivirus SARS-CoV-2.Hasil penelitian menunjukkan bahwa senyawa bioaktif minyak esensial akar dan biji hanjeli terdiri dari 40 senyawa yang terdapat pada minyak esensial akar Hanjeli dan 41 senyawa lagi berada di minyak esensial biji Hanjeli. Total keseluruhan senyawa sebanyak 81 dengan 19 senyawa merupakan senyawa bioaktif yang terdapat pada keduanya, sehingga didapati total sebesar 62 senyawa fitokimia yang berbeda-beda.Senyawa bioaktif minyak esensial akar hanjeli yang berguna sebagai antivirus ada sebanyak 9 senyawa. Pada minyak esensial biji ditemukan 11 senyawa yang memiliki bioaktivitas sebagai antivirus. Mekanisme kerja senyawa yang berpotensi sebagai antivirus umumnya sama, dimana setelah diidentifikasi pada software PASS online terdapat 2 mekanisme yakni sebagai RdRp Inhibitor dan sebagai 3Clpro inhibitor.
Aktivitas Biologis Minyak Esensial Daun dan Biji Jamblang (Syzygium cumini) Dan Potensinya Sebagai Antivirus SARS-CoV-2 Secara In Silico Fitriani, Sonia; Diningrat, Diky Setya; Sari, Ayu Nirmala; Harahap, Novita Sari; Kusdianti, Kusdianti
Kalwedo Sains (KASA) Vol 4 No 1 (2023): Kalwedo Sains (KASA), Maret 2023
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav4i1p17-30

Abstract

Penelitian ini bertujuan untuk mengetahui potensi senyawa antivirus SARS-CoV-2 dari minyak esensial daun dan biji Jamblang (Syzygium cumini) beserta mekanisme antivirusnya terhadap SARS-CoV-2 dengan pendekatan in silico. Minyak daun dan biji jamblang diperoleh melalui proses destilasi. Minyak hasil destilasi kemudian di analisis menggunakan Gas Chromatography Mass Spectophotometer (GC-MS). Hasil analisis GCMS dievaluasi meggunakan program MASSLAB. Data yang diperoleh dari alat GCMS kemudian dianalisis lanjut menggunakan software PubChem NCBI database (https://pubchem.ncbi.nlm.nih.gov/) dan PASS online. Hasil analisis GC-MS menunjukkan terdapat 45 senyawa bioaktif pada daun jamblang dan 69 senyawa bioaktif pada biji jamblang. Hasil analsis PubChem menunjukkan bahwa diperoleh sebanyak 10 senyawa yang memiliki aktivitas sebagai antiviral. Hasil analisis PASS online menunjukkan mekanisme antivirus sebagai 3Clpro inhibitor (3 C-like protease (Human coronavirus) inhibitor). Hasil penelitian ini dapat dijadikan landasan dalam program pengembangan pemanfaatan potensi senyawa bioaktif pada minyak esensial daun dan biji jamblang sebagai antivirus. Pada penelitian selanjutnya perlu dilakukan analisis pada bagian tanaman yang lain dan melakukan perbandingan untuk melengkapi database yang sudah tersedia.
Kandungan Metabolit pada Planlet Tebu (Saccharum officinarum L.) pada Perbedaan Media dan Frekuensi Subkultur Marhamah, Yualinda Durotul; Kusdianti, Kusdianti; Surakusumah, Wahyu
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 7, No 2 (2025): November 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v7i2.6509

Abstract

Sugarcane has been widely used to treat various diseases due to its metabolite content. Tissue culture is one method that can be used to produce metabolites and is an alternative biotechnology as a conservation strategy. This study aims to analyze the differences in metabolite content in sugarcane plantlets cultured with different media and subculture frequencies. Plantlets were extracted using the maceration method with 70% p.a. ethanol solvent. Metabolite analysis was carried out using a Gas Chromatography-Mass Spectrometry (GC-MS) instrument. Metabolite identification was carried out based on the NIST library. The results showed that the 10th subculture of sugarcane plantlets cultured on MS+Kinetin+Coconut Water media contained 5 metabolites, while the 11th subculture of plantlets cultured on MS+Kinetin+Tidiazuron media contained 7 metabolites. Differences in the type and number of metabolites indicate the influence of culture conditions on the metabolite profile of the plantlets. This study provides an initial overview of the potential of sugarcane tissue culture in the production of secondary metabolites.
Co-Authors Ayu Nirmala Sari Bangun, Adityana Arta Didik Priyandoko Diningrat, Diky Setya Diningrat, Diky Setya Eva Trisnawati Fitriani, Sonia Hafsah Hafsah Harahap, Novita Sari Hernawati Hernawati Juansah, Rahadian Deden Marhamah, Yualinda Durotul Mrs Sariwulan Diana Rahmadatillah, Fauziani Riandi Riandi Rini Solihat Sholihah, Ika Adhani Sinaga, Acong Jaya Sururi, Zaki Fahreza Topik Hidayat Wahyu Surakusumah WAHYU WIDOWATI Wardini, Trimurti H Zahira, Nurul Zahra