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All Journal Asian Journal of Agriculture Jurnal Jeumpa
Siregar, Anita Rasuna Sari
Samudra University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN LIDAH MERTUA (Sansevieria masoniana Chahin) DENGAN METODE DPPH(1,1-Difenil-2-Pikrilhidrazil) Siregar, Anita Rasuna Sari Siregar
Jurnal Jeumpa Vol 7 No 1 (2020): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v7i1.2552

Abstract

Sansevieria adalah salah satu tanaman hias yang memiliki aktivitas antioksidan. Salah satu spesies dari genus sansevieria adalah Sansevieria masoniana Chahin yang diduga memiliki aktivitas antioksidan . Aktivitas antioksidan speies sansevieria ini masih belum diketahui sehingga perlu dilakukan penelitian. Penelitian ini bertujuan untuk menguji aktivitas antioksidan ekstrak daun lidah mertua (Sansevieria masoniana Chahin). Ekstrak daun lidah mertua (Sansevieria masoniana Chahin) diperoleh melalui metode maserasi menggunakan pelarut etanol 96% . Ekstrak yang telah diuapkan pelarutya hingga menjadi ekstrak kental kemudian dilakukan skrining fitokimia, hasil skrining ekstrak menunjukkan positif mengandung flavonoid dan saponin. Kemampuan ekstrak sebagai antioksidan ditentukan melalui peredaman radikal DPPH (1,1- difenil-2-pikrihidrazil) terhadap variasi konsentrasi ekstrak 12,5 ppm, 25 ppm, 50 ppm, 100 ppm, dan 200 ppm. Hasil akhir dari uji DPPH tersebut ialah radikal berubah warna dari ungu menjadi kuning dan serapannya diukur dengan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Hasil uji aktivitas antioksidan yang dilakukan menghasilkan nilai IC50 sebesar 97,902 ppm yang menunjukkan bahwa aktivitas antioksidan ekstrak Sansevieria masoniana Chahin memiliki aktivitas antioksidan yang kuat.
In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal HARAHAP, FAUZIYAH; SIREGAR, ANITA RASUNA SARI; IDRAMSA, IDRAMSA; SURIANI, CICIK; EDI, SYAHMI; PANGGABEAN, NURUL HUDA; DAULAE, ABDUL HAKIM; POHAN, SELVIA DEWI; TANJUNG, AURA ALBIZIA; PERTIWI, SUFINA INTAN; KAIRANI, ANITA
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100100

Abstract

Abstract. Harahap F, Siregar ARS, Idramsa, Suriani C, Edi S, Panggabean NH, Daulae AH, Pohan SD, Tanjung AA, Pertiwi SI, Kairani A. 2026. In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal. Asian J Agric 10 (1): g100130. https://doi.org/10.13057/asianjagric/g100130. Cattleya sp. is an ornamental orchid whose seeds lack of endosperm, making natural germination and propagation difficult. In vitro culture using Protocorm-Like Bodies (PLBs) offers an alternative for mass propagation. This study aimed to evaluate the effects of coconut water and activated charcoal on the in vitro regeneration of Cattleya sp. PLBs. A completely randomized design was employed with varying concentrations of coconut water (0, 5, and 10%) and activated charcoal (0, 0.1, 0.2, and 0.3 g/L). Morphological parameters observed included time to shoot emergence, number of leaves, shoots, and roots, as well as shoot color. Data were analyzed using two-way ANOVA. The results showed that coconut water significantly accelerated shoot emergence with 10% of coconut water producing the fastest response (12 days after treatment). Activated charcoal enhanced shoot and leaf formation with 0.3 g/L, producing the highest number of shoot and leaf. Root formation was optimal at lower charcoal concentrations. Overall, the combination of coconut water and activated charcoal significantly influenced PLBS growth and regeneration. These findings indicate that appropriate combinations of organic additives can optimize in vitro regeneration of Cattleya sp. and support efficient orchid propagation.
Co-Authors CICIK SURIANI Daulae, Abdul Hakim Fauziyah Harahap Idramsa Idramsa, Idramsa Kairani, Anita Panggabean, Nurul Huda PERTIWI, SUFINA INTAN Selvia Dewi Pohan Syahmi Edi Tanjung, Aura Albizia