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Laboratory Examinations in the Diagnosis of Dengue Hemorrhagic Fever: A Systematic Literature Review Sangging, Putu Ristyaning Ayu; Prasetyo, Muhammad Purwono; Shiddiq, Muhammad Nasrullah Nur; Aprilia, Intan Rahma; Mukhbita, Khalila Alya; Zahra, Siti Aqila
Jurnal Kesehatan Metro Sai Wawai Vol. 18 No. 2 (2025): Jurnal Kesehatan Metro Sai Wawai
Publisher : Poltekkes Kemenkes Tanjung Karang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jkmsw.v18i2.5434

Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and remains a major public health problem in Indonesia. Early and accurate diagnosis is crucial for determining appropriate management and preventing fatal complications. This systematic literature review aimed to evaluate and synthesize evidence on laboratory diagnostic methods for DHF across Southeast Asia. A comprehensive search was conducted across Pubmed, ScienceDirect, Scopus, Google Scholar and DOAJ for studies published between 2015 and 2025, using keywords such as “Dengue Hemorrhagic Fever,” “Laboratory Diagnosis,” and “Southeast Asia.” Inclusion criteria encompassed peer-reviewed studies reporting empirical on hematological, serological, or molecular laboratory test for DHF, while reviews without empirical finding studies unrelated to dengue diagnosis were excluded. Found on database 40 after screening, 22 studies met the inclusion criteria and were analyzed. NS1 antigen testing plays a crucial role in the early phase of infection, while IgM and IgG serological tests are useful in identifying the immunological phase of the disease. Meanwhile, RT-PCR is a highly sensitive method for directly detecting the presence of the virus. Combining several testing methods has been shown to improve diagnostic accuracy, both in terms of sensitivity and specificity. However, optimal implementation of these tests still faces challenges such as limited resources, cost, time, and differences in laboratory capabilities at various levels of health facilities. Therefore, strengthening laboratory capacity, standardizing testing methods, and implementing a combination testing strategy are needed to improve the reliability of dengue diagnosis. This approach is expected to support early detection, accelerate treatment, and strengthen efforts to control dengue outbreaks nationally.
Education on Sedentary Lifestyle, Nutritional Status, and Metabolic Syndrome Among Students Prasetyo, Muhammad Purwono; Wardhana, Carbela Zahra; Lestari, Dharma Lina; Putri, Nandini Amerta; Hening, Putik Titian Citra; Islami, Suryadi
Jurnal Abmas Negeri (JAGRI) Vol. 6 No. 2 (2025): Volume 6 Nomor 2 Desember 2025
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jagri.v6i2.1798

Abstract

Sedentary lifestyle and imbalanced nutritional status are major risk factors for metabolic syndrome, including among adolescents. Currently, many adolescents experience a lack of physical activity due to various factors, including excessive use of gadgets, prolonged sitting during study, and unhealthy eating habits. This community service activity aimed to educate students of Ar-Raihan Boarding School, Lampung, regarding sedentary lifestyle, nutritional status, and the prevention of metabolic syndrome. The methods included health promotion through interactive counseling, simple nutritional status assessment using Body Mass Index (BMI) measurement, blood pressure examination, and random blood glucose examination, as well as group discussions. The health education activities were successfully implemented and received positive responses from students. Screening results showed variations in nutritional status and health conditions that served as the basis for providing educational feedback. After the intervention, students demonstrated increased understanding of sedentary lifestyle risk factors and the importance of adopting a healthy lifestyle to prevent metabolic syndrome. This health education program is expected to be implemented sustainably to support the development of healthy behaviors from adolescence.