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Dea Sarayar
Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Antagonist Test of Gliocladium sp. against Fusarium sp. Cause of Wilt Disease in Shallot Plants In Vitro Dea Sarayar; Stella Deiby Umboh; Parluhutan Siahaan
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62836

Abstract

This study aims to analyze the morphological characteristics of the fungus Gliocladium sp., measure its inhibition against the growth of the fungus Fusarium sp. causing wilt disease in shallot plants (Allium ascalonicum L), and examine its antagonistic mechanism in vitro. This research method includes exploration and sampling, sterilization of tools and materials, making PDA media, isolation, purification, and identification of morphological characteristics and measurement of antagonistic fungal inhibition. The antagonist test uses the dual culture method, and data analysis with a completely randomized design (CRD) and ANOVA test followed by the Honestly Significant Difference (HSD) Test at the 5% significance level. The results showed that Gliocladium sp. was able to inhibit the growth of Fusarium sp. with a percentage inhibition of 25.63% and spore viability of 66.52%. Antagonistic mechanisms observed include competition for space and nutrients, antibiosis, lysis, and mycoparasitism.
Co-Authors Parluhutan Siahaan Stella Deiby Umboh