Jurnal Bios Logos
Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS

Antagonist Test of Gliocladium sp. against Fusarium sp. Cause of Wilt Disease in Shallot Plants In Vitro

Dea Sarayar (Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University)
Stella Deiby Umboh (Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University)
Parluhutan Siahaan (Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University)



Article Info

Publish Date
30 Aug 2025

Abstract

This study aims to analyze the morphological characteristics of the fungus Gliocladium sp., measure its inhibition against the growth of the fungus Fusarium sp. causing wilt disease in shallot plants (Allium ascalonicum L), and examine its antagonistic mechanism in vitro. This research method includes exploration and sampling, sterilization of tools and materials, making PDA media, isolation, purification, and identification of morphological characteristics and measurement of antagonistic fungal inhibition. The antagonist test uses the dual culture method, and data analysis with a completely randomized design (CRD) and ANOVA test followed by the Honestly Significant Difference (HSD) Test at the 5% significance level. The results showed that Gliocladium sp. was able to inhibit the growth of Fusarium sp. with a percentage inhibition of 25.63% and spore viability of 66.52%. Antagonistic mechanisms observed include competition for space and nutrients, antibiosis, lysis, and mycoparasitism.

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Journal Info

Abbrev

bioslogos

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ...