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All Journal Samakia: Jurnal Ilmu Perikanan Jurnal MediLab Mandala Waluya
Satriani Syarif
Universitas Mandala Waluya
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Other

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Penapisan Fitokimia Infusa Daun Thalassia hemprichii yang Berpotensi Sebagai Antimikroba Suwarny Suwarny; Satriani Syarif; La Ode Alrin
Samakia : Jurnal Ilmu Perikanan Vol 15 No 2 (2024): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/jsapi.v15i2.5866

Abstract

Thalassia hemprichii is a spermatophyte plant that grows in aquatic ecosystems. This plant is a type of seagrass widely distributed in the waters of Southeast Sulawesi and has long been utilized by coastal communities for various purposes, including traditional medicine. This study aims to conduct phytochemical screening of Thalassia hemprichii infusion from the waters of Tondonggeu, Southeast Sulawesi, and to assess its potential as a natural antimicrobial agent. The methods used include interviews with local communities to explore traditional knowledge about seagrass utilization, as well as phytochemical screening of Thalassia hemprichii leaf infusion. The interview results show that the Tondonggeu community utilizes various parts of the seagrass plant for medicinal purposes, including as a wound treatment, antidiarrheal, fever medicine, stomach ache remedy, and nutritional supplement. Phytochemical screening revealed the presence of alkaloids, terpenoids, saponins, tannins, and flavonoids in the T. hemprichii leaf infusion. These findings indicate a correlation between traditional uses and phytochemical content, supporting the potential of T. hemprichii as a natural antimicrobial source. This study concludes that Thalassia hemprichii has significant potential for further development as a source of natural medicine, especially in antimicrobial discovery. However, further research is needed to isolate specific active compounds and conduct clinical trials to validate its safety and efficacy. The utilization of T. hemprichii as a source of natural medicine must be balanced with conservation efforts to ensure the sustainability of this species and the seagrass ecosystem. Keywords: Thalassia hemprichii, phytochemical screening, antimicrobial
PERBANDINGAN LAMA PENYIMPANAN SAMPEL HASIL ISOLASI DNA MENGGUNAKAN METODE CTAB (Cetil Trimetil Ammonium Bromide) UNTUK MENDETEKSI MUTASI HETEROPLASMI PADA PENDERITA DIABETES MELITUS Satriani Syarif; La Ode Hamiru; Abdi Handayani
Jurnal MediLab Mandala Waluya Vol. 10 No. 1 (2026): JURNAL MEDILAB MANDALA WALUYA
Publisher : Prodi D4 Teknologi Laboratorium Medis, Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/medilab.v10i1.1737

Abstract

Diabetes Melitus (DM) adalah kadar glukosa tinggi dari normal karena tubuh tidak mensekresi insulin dengan baik. Lama penyimpanan hasil isolasi DNA pada sampel DNA yang telah diisolasi dapat disimpan tanpa mengalami degradasi. Penelitian ini bertujuan untuk mendeteksi mutasi heteroplasmi menggunakan metode CTAB pada sampel langsung dan sampel lama penyimpanan 6 bulan pada penderita diabetes melitus.  Jenis penelitian ini dilakukan secara deskriptif. Populasi dan sampel penelitian ini adalah hasil isolasi DNA dengan jumlah 7 responden. Metode yang digunakan dalam penelitian ini adalah Amplification Refractory Mutation System (ARMS PCR). Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa ada perbedaan antara hasil elektroforesis isolasi DNA menggunakan metode CTAB langsung didapatkan sampel hasil mutasi heteroplasmi 5 responden dari 7 reponden. Sedangkan hasil elektroforesis isolasi DNA menggunakan metode ARMS PCR dengan lama penyimpanan sampel 6 bulan didapatkan sampel normal 7 responden. Kesimpulan ada perbedaan antara hasil elektroforesis isolasi DNA menggunakan metode CTAB langsung didapatkan sampel didapatkan mutasi heteroplasmi 5 responden dari 7 responden. Sedangkan hasil elektroforesis isolasi DNA menggunakan metode ARMS PCR dengan lama penyimpanan sampel 6 bulan didapatkan hasil normal 7 responden. Saran adapun saran dalam penelitian ini diharapkan kepada peneliti selanjutnya untuk mendeteksi dengan metode ini dengan sampel penyakit lain.
Co-Authors Abdi Handayani La Ode Alrin La Ode Hamiru Suwarny Suwarny