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All Journal Bioma : Jurnal Biologi Indonesia Quagga: Jurnal Pendidikan dan Biologi
Nurul Mahmudati
Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Concentration and Staining Duration Effects of Clitoria ternatea Extract on Wholemount Preparate Quality Isna Putri Arifa; Eko Susetyarini; Nurul Mahmudati; Sri Wahyuni
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 2 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i2.509

Abstract

Making intact preparations to observe the anatomical structure of plants and animals in biological practicum activities requires effective dyes. Synthetic dyes such as safranin are commonly used, but they are relatively expensive, carcinogenic, and less environmentally friendly. This study aimed to examine the effect of variations in concentration and duration of staining using telang flower extract (Clitoria ternatea) on the quality of the whole plant preparation Hydrocotyle umbellata. The study was conducted experimentally with a combination of concentration treatment (70%, 80%, and 90%) and duration of staining (15 and 30 minutes). The quality of the preparations was assessed using assessment parameters which included clarity of anatomical structure, colour contrast and colour distribution. The data obtained were analyzed to determine the best treatment combination, as well as compared with safranin-stained and no-staining control preparations. Results showed that increasing concentration and duration of staining had an effect on the quality of the preparation results. The combination of concentrations of 80% of the 15-minute duration of staining gave the best results with the identification of trichomes, strong color contrast and even color. Based on these results, telang flower extract has the potential to be used as a natural dye alternative to safranin which is safer, more economical and more environmentally friendly.
Invertebrate diversity in the intertidal zone of Lombang beaches, Sumenep, Madura according to area utilization Vikry Nurul Iman Iman; Lise Chamisijatin; Nurul Mahmudati
Bioma Vol. 21 No. 1 (2025): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/bioma.v21i1.49823

Abstract

Pantai Lombang had experienced many human-induced environmental pressure, presently from tourist activities, which may lower the coastal biotic biodiversity, an important aspect for the livelihood of local inhabitants. Some groups of animals may directly be influenced by the activities, especially some non motile marine invertebrates which are prone to recreational activities and/or tourist accidental picking.  The purpose of this study is to find the group of invertebrates in the intertidal zone and describe the diversity of invertebrates in the intertidal zone in Lombang Beach, Sumenep Regency. The research method uses belt transect at three differing stations: the tourist area, fishpond area and a least utilized area. The result showed that the invertebrates in the least utilized area are higher in diversity and evenness index compared to two other areas. The least utilized area also inhabited with more species compared to two other area. This condition may relate to the higher DO in the area, compared to two other area.  This result may be important in the management of biodiversity on utilized beaches area in Indonesia.
Co-Authors Eko Susetyarini Isna Putri Arifa Lise Chamisijatin Sri Wahyuni Vikry Nurul Iman Iman